为理解长期有机物料与化学氮肥配施对土壤有机碳和全氮的影响，在黄淮海平原开展了长达30年的小麦-玉米轮作田间试验（1990–2019年）。试验包含5个施肥处理：不施肥（control）、单施化肥（NPK）、化肥与秸秆配施（NPKS）、化肥与有机肥配施（NPKM）和1.5倍化肥与有机肥配施（1.5NPKM），NPK、NPKS和NPKM处理氮投入总量相同。测定试验期间作物产量，采集土壤表层(0–10 and 10–20 cm)和下层(20–40 cm)样品，进行土壤团聚体分级并测定团聚体碳氮含量。有机无机配合处理（NPKS, NPKM和1.5NPKM）与NPK处理相比，30年平均作物产量无显著差异，土壤表层和下层有机碳和全氮均显著提高，表层土壤有机碳和全氮分别提高24.1–44.4%和22.8–47.7%，下层土壤有机碳和全氮分别提高22.0–47.9%和19.8–41.8%%。与NPK处理相比，NPKS处理0-10cm土层和NPKM处理20-40cm土层具有显著较高的大团聚体组分质量比例，提高幅度分别为19.8和27.0%；然而，1.5NPKM处理在0-10cm和20-40cm土层的土壤大团聚体组分质量比例较对照显著降低，下降幅度分别为-19.2和-29.1%。分析表明有机无机配合处理较NPK处理有显著较高土壤有机碳和全氮主要与自由态微团聚体和大团聚体中的微团聚体等土壤稳定性组分的碳氮增加有关，稳定性组分对土壤碳氮含量增加的贡献率分别达81.1–91.7%和 83.3–94.0%。不同施肥处理0-40cm土层的土壤稳定性碳库和氮库与处理年平均碳投入量均呈显著正相关，回归系数分别为0.74和0.72（P<0.01），表明土壤氮对碳贮存的重要性。我们研究为长期有机物料与化学氮肥配施措施在保持合理总氮投入下有利于上层和下层土壤碳氮的保蓄提供了证据。

Received  27 October, 2017    Accepted  11 January, 2018

Dissimilatory Fe(III) reduction is an important process in the geochemical cycle of iron in anoxic environment.  As the main products of dissimilatory iron reduction, the Fe(II) species accumulation could indicate the reduction ability.  The effects of different green manures on Fe(III) reduction in paddy soil were explored based on a 31-year rice-rice-winter green manure cropping experiment.  Four treatments were involved, i.e., rice-rice-milk vetch (RRV), rice-rice-rape (RRP), rice-rice-ryegrass (RRG) and rice-rice-winter fallow (RRF).  Soils were sampled at flowering stage of milk vetch and rape (S1), before transplantation (S2), at tillering (S3), jointing (S4), and mature (S5) stages of the early rice, and after the harvest of the late rice (S6).  The contents of TFe_HCl (HCl-extractable total Fe), Fe(II)_HCl (HCl-extractable Fe(II) species) and Fe(III)_HCl (HCl-extractable Fe(III) species) were measured.  The correlations among those Fe species with selected soil environmental factors and the dynamic characteristics of Fe(II)_HCl accumulation were investigated.  The results showed that TFe_HCl in RRF was significantly higher than those in the green manure treatments at most of the sampling stages.  Fe(II)_HCl increased rapidly after the incorporation of green manures in all treatments and kept rising with the growth of early rice.  Fe(II)_HCl in RRG was quite different from those in other treatments, i.e., it reached the highest at the S2 stage, then increased slowly and became the lowest one at the S4 and S5 stages.  Fe(III)_HCl showed oppositely, and Fe(II)_HCl/Fe(III)_HCl performed similarly to Fe(II)_HCl.  The maximum accumulation potential of Fe(II)_HCl was significantly higher in RRF, while the highest maximum reaction rate of Fe(II)_HCl accumulation appeared in RRG.  Significant correlations were found between the indexes of Fe(II)_HCl accumulation and soil pH, oxidation-reduction potential (Eh) and total organic acids, respectively.  In together, we found that long-term application of green manures decreased the TFe_HCl in red paddy soils, but promoted the ability of Fe(III) reduction, especially the ryegrass; Fe(II)_HCl increased along with the growth of rice and was affected by soil conditions and environmental factors, especially the water and redox ability.

On the basis of a long-term (30 years) field experiment that involved four rotation systems, rice-rice-winter fallow (RRF), rice-rice-ryegrass (RRG), rice-rice-rape (RRP), and rice-rice-milk vetch (RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16S rRNA gene. The Chao1 richness and non-parametric Shannon’s index increased in all soil samples that received green manure treatments. The communities’ structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.

The development of more efficient management systems is crucial to achieving high grain yields with high nitrogen use efficiency (NUE). February Orchid-spring maize rotation system is a newly established planting system with the benefits of ground cover and potential wind erosion in northern China. A field experiment was conducted to evaluate the effects of integrated application of February Orchid as green manure with reduction of chemical fertilizers (INTEGRATED) on spring maize yield, N uptake, ammonium volatilization, and soil residual mineral N in northern China. Compared to farmers’ traditional fertilization (CON), integrated application of February Orchid as green manure with 30% reduction of nitrogen fertilizers (INTEGRATED) increased maize grain yield and biomass by 9.9 and 10.2%, respectively. The 0–100 cm soil residual Nmin at harvest was decreased by 58.5% and thus nitrogen use efficiency was increased significantly by 26.7%. The nitrogen balance calculation further demonstrated that the INTEGRATED approach performed better than CON with lower apparent nitrogen loss (decreased by 48.9%) which evidenced by the ammonium volatilization of top-dressing fertilizer was decreased by 31.1%, the Nmin movement to the deeper soil layers was reduced, and the apparent nitrogen leaching loss nearly equal to 0 under the INTEGRATED treatment. Therefore, in northern China, integrated application of green manure and chemical fertilizers is an efficient management approach for improving maize yields and NUE simultaneously.