Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China

doi:10.1016/S2095-3119(15)61230-8

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (12): 2512-2520.DOI: 10.1016/S2095-3119(15)61230-8

Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China

GAO Song-juan, ZHANG Ren-gang, CAO Wei-dong, FAN Yuan-yuan, GAO Ju-sheng, HUANG Jing, BAI Jin-shun, ZENG Nao-hua, CHANG Dan-na, Shimizu Katsu-yoshi, Kristian Thorup-Kristensen

1、Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning,
Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、The Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3、College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R.China
4、Soil and Fertilizer Institute, Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, P.R.China
5、College of Resources and Environmental Sciences, China Agricultural University, Beijing 100083, P.R.China
6、Red Soil Experimental Station in Hengyang, Chinese Academy of Agricultural Sciences, Qiyang 426182, P.R.China
7、Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan
8、Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen 2630, Denmark

收稿日期:2015-08-21 出版日期:2015-12-01 发布日期:2015-12-16
通讯作者: CAO Wei-dong, Tel: +86-10-82109622,E-mail: caoweidong@caas.cn
作者简介:GAO Song-juan, E-mail: gaosongjuan@caas.cn;* These authors contributed equally to this study.
基金资助:
This study was supported by the Special Fund for Agro-scientific Research in the Public Interest of China (201103005) and the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (2013–2017)

Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China

GAO Song-juan, ZHANG Ren-gang, CAO Wei-dong, FAN Yuan-yuan, GAO Ju-sheng, HUANG Jing, BAI Jin-shun, ZENG Nao-hua, CHANG Dan-na, Shimizu Katsu-yoshi, Kristian Thorup-Kristensen

1、Key Laboratory of Plant Nutrition and Fertilizer, Ministry of Agriculture/Institute of Agricultural Resources and Regional Planning,
Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
2、The Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R.China
3、College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, P.R.China
4、Soil and Fertilizer Institute, Qinghai Academy of Agriculture and Forestry Sciences, Qinghai University, Xining 810016, P.R.China
5、College of Resources and Environmental Sciences, China Agricultural University, Beijing 100083, P.R.China
6、Red Soil Experimental Station in Hengyang, Chinese Academy of Agricultural Sciences, Qiyang 426182, P.R.China
7、Faculty of Agriculture, Kagoshima University, Kagoshima 890-0065, Japan
8、Department of Plant and Environmental Sciences, University of Copenhagen, Copenhagen 2630, Denmark

Received:2015-08-21 Online:2015-12-01 Published:2015-12-16
Contact: CAO Wei-dong, Tel: +86-10-82109622,E-mail: caoweidong@caas.cn
About author:GAO Song-juan, E-mail: gaosongjuan@caas.cn;* These authors contributed equally to this study.
Supported by:
This study was supported by the Special Fund for Agro-scientific Research in the Public Interest of China (201103005) and the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (2013–2017)

摘要/Abstract

摘要： On the basis of a long-term (30 years) field experiment that involved four rotation systems, rice-rice-winter fallow (RRF), rice-rice-ryegrass (RRG), rice-rice-rape (RRP), and rice-rice-milk vetch (RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16S rRNA gene. The Chao1 richness and non-parametric Shannon’s index increased in all soil samples that received green manure treatments. The communities’ structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.

关键词: green manure , community structure , community function , 16S rRNA gene , double rice

Abstract: On the basis of a long-term (30 years) field experiment that involved four rotation systems, rice-rice-winter fallow (RRF), rice-rice-ryegrass (RRG), rice-rice-rape (RRP), and rice-rice-milk vetch (RRV), this study described the effects of green manure on the microbial communities in the red paddy soils using 454 pyrosequencing for the 16S rRNA gene. The Chao1 richness and non-parametric Shannon’s index increased in all soil samples that received green manure treatments. The communities’ structures with the green manure applications were significantly dissimilar from that under the winter fallow. Using Metastats tests, many genera in the RRG, RRP and RRV soils were significantly different from those in the RRF soil, including a number of genera that functioned in the nitrogen and sulfur cycles. Analyses of the genera with these functions revealed the shifts in microbial ecosystem functions after long-term green manuring. Changes in the microbial communities increased the ammonium supply and decreased the soil acidification in green-manure-amended soils. Together, these data suggested powerful effects of green manure on both the microbial communities and the biogeochemical cycle driven by the shifts in bacterial functional groups.

Key words: green manure , community structure , community function , 16S rRNA gene , double rice

GAO Song-juan, ZHANG Ren-gang, CAO Wei-dong, FAN Yuan-yuan, GAO Ju-sheng, HUANG Jing, BAI Jin-shun, ZENG Nao-hua, CHANG Dan-na, Shimizu Katsu-yoshi, Kristian Thorup-Kristensen. Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China[J]. Journal of Integrative Agriculture, 2015, 14(12): 2512-2520.

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Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China

Long-term rice-rice-green manure rotation changing the microbial communities in typical red paddy soil in South China

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