The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis

doi:10.1016/S2095-3119(13)60495-5

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (1): 105-114.DOI: 10.1016/S2095-3119(13)60495-5

The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis

YANG Xing, ZHANG Li-hui, SHI Cui-ping, SHANG Yan, ZHANG Jin-lin, HAN Jian-min , DONG

Molecular Plant Pathology Laboratory, College of Life Science, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2012-12-13 出版日期:2014-01-01 发布日期:2014-01-04
通讯作者: DONG Jin-gao, Tel: +86-312-7528266, Fax: +86-312-7528142, E-mail: dongjingao@126.com
作者简介:YANG Xing, Tel: +86-311-83521567, E-mail: x.yang14@newcastle.ac.uk
基金资助:
This research was supported by the Natural Science Foundation of Hebei Province, China (C2013204115) and the Science and Technology Support of Hebei Province, China (C20121124001).

The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis

YANG Xing, ZHANG Li-hui, SHI Cui-ping, SHANG Yan, ZHANG Jin-lin, HAN Jian-min , DONG

Molecular Plant Pathology Laboratory, College of Life Science, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2012-12-13 Online:2014-01-01 Published:2014-01-04
Contact: DONG Jin-gao, Tel: +86-312-7528266, Fax: +86-312-7528142, E-mail: dongjingao@126.com
About author:YANG Xing, Tel: +86-311-83521567, E-mail: x.yang14@newcastle.ac.uk
Supported by:
This research was supported by the Natural Science Foundation of Hebei Province, China (C2013204115) and the Science and Technology Support of Hebei Province, China (C20121124001).

摘要/Abstract

摘要： Large amounts of Flaveria bidentis’s root culturing solution were obtained by using DFT (deep flow technique) equipment and these solution which was vacuum concentrated (10, 20 mg mL-1) can have a certain inhibition on Triticum aestivum, Cucumis sativus, Raphanus sativus, Amaranthus retroflexus, Setaria viridis, Chenopodium album, Echinochloa crusgalli and Chloris virgata. This outcome suggested some active compounds in the root exudates of Flaveria bidentis can inhibit the germination, seedling elongation and root length. The dichloromethane extract of root exudates was identificated by GC-MS, and 29 kinds of compounds, including esters, hydrocarbons, ketones, thiazole, amines, etc. were obtained and the phthalate n-octyl ester, phthalate 2-ethylhexyl ester were proved to be allelochemicals. The culturing solution of root exudates was separated through the resin column and silica gel column and a component inhibiting seedling height, root length and fresh weight of wheat was got. There were 6 kinds of organic compounds in this component including dioctyl phthalate, 1,2-phthalate, mono(2-ethylhexyl) ester by GC-MS.

关键词: extraction , isolation , identification , exudates , Flaveria bidentis

Abstract: Large amounts of Flaveria bidentis’s root culturing solution were obtained by using DFT (deep flow technique) equipment and these solution which was vacuum concentrated (10, 20 mg mL-1) can have a certain inhibition on Triticum aestivum, Cucumis sativus, Raphanus sativus, Amaranthus retroflexus, Setaria viridis, Chenopodium album, Echinochloa crusgalli and Chloris virgata. This outcome suggested some active compounds in the root exudates of Flaveria bidentis can inhibit the germination, seedling elongation and root length. The dichloromethane extract of root exudates was identificated by GC-MS, and 29 kinds of compounds, including esters, hydrocarbons, ketones, thiazole, amines, etc. were obtained and the phthalate n-octyl ester, phthalate 2-ethylhexyl ester were proved to be allelochemicals. The culturing solution of root exudates was separated through the resin column and silica gel column and a component inhibiting seedling height, root length and fresh weight of wheat was got. There were 6 kinds of organic compounds in this component including dioctyl phthalate, 1,2-phthalate, mono(2-ethylhexyl) ester by GC-MS.

Key words: extraction , isolation , identification , exudates , Flaveria bidentis

YANG Xing, ZHANG Li-hui, SHI Cui-ping, SHANG Yan, ZHANG Jin-lin, HAN Jian-min , DONG. The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis[J]. Journal of Integrative Agriculture, 2014, 13(1): 105-114.

YANG Xing, ZHANG Li-hui, SHI Cui-ping, SHANG Yan, ZHANG Jin-lin, HAN Jian-min , DONG . The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis[J]. Journal of Integrative Agriculture, 2014, 13(1): 105-114.

参考文献

Bais H P, Vepachedu R, Gilroy S, Callaway R M, Vivanco J M. 2003. Allelopathy and exotic plant invasion: from molecules and genes to species interactions. Science, 301, 1377-1380

Bi H H, Zeng R S, Su L M, An M, Luo S M. 2007. Rice allelopathy induced by methyl jasmonate and methyl salicylate. Journal of Chemistry Ecology, 33, 1089-1103

von Caemmerer S, Hendrickson L, Quinn V, Vella N, Millgate A G, Furbank R T. 2005. Reductions of Rubisco activase by antisense RNA in the C4 plant Flaveria bidentis reduces Rubisco carbamylation and leaf photosynthesis. Plant Physiology, 137, 747-755

Callaway R M, Aschehoug E T. 2000. Invasive plants versus their new and old neighbors: a mechanism for exotic invasion. Science, 290, 521-523

Engelmann S, Wiludda C, Burscheidt J, Gowik U, Schlue U, Koczor M, Streubel M, Cossu R, Bauwe H, Westhoff P, 2008. The gene for the P-subunit of glycine decarboxylase from the C4 species Flaveria trinervia: analysis of transcriptional control in transgenic Flaveria bidentis (C4) and Arabidopsis (C3). Plant Physiology, 146, 1773-1785

Han L, Shen Q, Wang S, Yang Z. 2002, Identification on decomposing products of soybean stubs by Trichodermar koningii and their allelopathy. Chinese Journal of Applied Ecology, 13, 1295-1299

Hao F, Hong C, Chen H G, Liu X Y, Wang M D. 2010. Bioactivity of root exudates of rehmannia glutinosa and identification of their allelopathic compounds. Acta Agriculturae Jiangxi, 3, 131-134

Huang H J, Zhang C X, Meng Q H, Wei S H, Liu Y, Cui H L. 2008. Allelopathic potential of invasive alien weed Sorghum halepense (L.) Pers. Chinese Journal of Ecology, 27, 1234-1237 (in Chinese)

Jia C Q, Li Y C, Tu P F, Zhang H Q. 2005. Study on the pretreatment of the series of HPD macroporous adsorption resin. China Journal of Chinee Materia Medica, 18, 1425- 1427.

Jia C Q, Li Y C, Tu P F, Zhang H Q. 2006. Pretreatment of D-101 macroporous adsorption resin Chinese Traditional and Herbal Drugs, 37, 193-196. (in Chinese)

Jin Z H, Zhuang Y Y, Dai S G, Li T L. 2003. Isolation and identification of extracts of Eichhornia crassipes and their allelopathic effects on algae. Bulletion Environmental Contamination and Toxicology, 71, 1048-1052

Li J H, Mu J L, Guo H Y, Wang X R, Yin T Q. 2005. Effects of dibutyl phthalate (DBP) pollution on the physiological index of Vallisneria spiralis L. Environmental Chemistry, 1, 31-35 (in Chinese)

Li H N, Liu W X, Wan F H, Cao Y Y. 2009. Comparison of impacts of invasive weed, Ambrosia artemisiifolia, and native weed, Digitaria chinensis on soil enzyme activity and fertility. Chinese Journal of Eco-Agriculture, 17, 847-850

Li K, Guo X W, Guo Y S, Li C X, Xie H G, Hu X X, Zhang L H, Sun Y N. 2010. Allelopathy of grape root aqueous extracts. Chinese Journal of Applied Ecology, 21, 1779- 1784. (in Chinese)

Lu P, Liang H, Wang H Y, Bai Y M, Gao F J, Song G, Wu Y, Tian Q Y. 2010. Research progress on exotic invasive weed Amaranthus retroflexus. Chinese Journal of Ecology, 8, 1662-1670

Murrell C, Gerber E, Krebs C, Parepa M, Schaffner U, Bossdorf O. 2011. Invasive knotweed affects native plants through allelopathy. American Journal of Botany, 98, 38-43

Patel M, Corey A C, Yin L P, Ali S, Taylor W C, Berry J O. 2004. Untranslated regions from C4 amaranth AhRbcS1 mRNAs confer translational enhancement and preferential bundle sheath cell expression in transgenic C4 Flaveria bidentis. Plant Physiology, 136, 3550-3561

Pratt R. 1962. Effect of ionized air on early growth of black mustard seedlings. Journal of Pharmaceutical Sciences, 51, 184-185

Rasher D B, Hay M E. 2010. Seaweed allelopathy degrades the resilience and function of coral reefs. Communicative & Integrative Biology, 3, 564-566

Seal A N, Pratley J E, Haig T, An M. 2004. Identification and quantitation of compounds in a series of allelopathic and non-allelopathic rice root exudates. Journal of Chemistry Ecology, 30, 1647-1662

Tang C S, Young C C. 1982. Collection and identification of allelopathic compounds from the undisturbed root system of Bigalta limpograss (Hemarthria altissima). Plant Physiology, 69, 155-160

Tseng M H, Lai W R, Hsieh C L, Kuo Y H. 2007. Allelopathy on bark of downed logs of Chamaecyparis obtusa Sieb. and Zucc. var. formosana (Hayata) Rehder. Journal of Chemistry Ecology, 33, 1283-1296

Tang X L, Tan W Z, Fu W D, Zhang G L, Chen Y. 2010. Biological characters and integrated control of the alien invasive weed Flaveria bidentis. Journal of Hunan Agricultural University (Natural Sciences), 6, 694-699

Wang J X. 2008. Development of minitype pipe DFT system. Hubei Agricultural Sciences, 1, 101-103 (in Chinese)

Wang Q X, Zhang H J, Guo M X, Meng X Q, Peng L, Jin S K, Yang J S, Cao A C. 2008. Chemistry control of invasive weed Flaveria bidentis (L.) Kuntze. Ecolology and Environment, 3, 1184-1189. (in Chinese)

Xu M M, Hillwig M L, Prisic S, Coates R M, Peters R J, 2004. Functional identification of rice syn-copalyl diphosphate synthase and its role in initiating biosynthesis of diterpenoid phytoalexin/allelopathic natural products. The Plant Journal, 39, 309-318

Zhou Z H, Luo S M, Mou Z P. 1998. Study on allelopathic potentials of several chemical compounds of tomato (Lycopersicon). Journal of South China Agricultural University, 3, 59-63. (in Chinese)

Zhu X, Zhang J, Ma K. 2011. Soil biota reduce allelopathic effects of the invasive Eupatorium adenophorum. PLoS ONE, 6, e25393.

[1]	LI Yu-yuan, YANG Rui, GAO Ru, WEI Hong-an, CHEN An-lei, LI Yong. Effects of long-term phosphorus fertilization and straw incorporation on phosphorus fractions in subtropical paddy soil[J]. Journal of Integrative Agriculture, 2015, 14(2): 365-373.
[2]	HUO Jing-qian, XING Ji-hong, ZHANG Li-hui, KANG Zhan-hai , ZHANG Jin-lin. Isolation and Structural Identification of Herbicidal Active Substance from Root of Flaveria bident (L.) Kuntze[J]. Journal of Integrative Agriculture, 2014, 13(4): 804-810.
[3]	YANG Xiu-shi, WANG Li-jun, DONG Chuan, Edmund Man King Lui , REN Gui-xing. Optimization of Ultrasonic-Assisted Extraction Process of Polysaccharides from American Ginseng and Evaluation of Its Immunostimulating Activity[J]. Journal of Integrative Agriculture, 2014, 13(12): 2807-2815.
[4]	CHEN Xian-you, WU Liang-huan, CAO Xiao-chuang, Sarkar Animesh, ZHU Yuan-hong. An Experimental Method to Quantify Extractable Amino Acids in Soils from Southeast China[J]. Journal of Integrative Agriculture, 2013, 12(4): 732-736.
[5]	ZHAO Te, GAO Fei, ZHOU Lin, SONG Tian-you. Essential Oil from Inula britannica Extraction with SF-CO2 and Its Antifungal Activity[J]. Journal of Integrative Agriculture, 2013, 12(10): 1791-1798.
[6]	LI Jing, LIU Xin-gang, DONG Feng-shou, XU Jun, GUO Li-qun, KONG Zhi-qiang, TIAN Ying-ying, WU Yan-bin , ZHENG Yong-quan. ASimpleMethod for the Isolation andPurification of 2,4-Dihydroxy-7-Methoxy- 2H-1,4-Benzoxazin-3(4H)-One (DIMBOA) from Maize (Zea mays L.) Seedlings[J]. Journal of Integrative Agriculture, 2013, 12(1): 95-102.
[7]	ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam, LI Xiang-rui. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China[J]. Journal of Integrative Agriculture, 2012, 12(8): 1347-1353.
[8]	ZHENG Wen-li, ZHANG Li-fang, ZHANG Ke-yu, WANG Xiao-yang, XUE Fei-qun. Determination of Tetracyclines and Their Epimers in Agricultural Soil Fertilized with Swine Manure by Ultra-High-Performance Liquid Chromatography Tandem Mass Spectrometry[J]. Journal of Integrative Agriculture, 2012, 12(7): 1189-1198.
[9]	HU Zhi-yong, ZHAN Gao-miao, WANG Han-zhong, HUA Wei. A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue[J]. Journal of Integrative Agriculture, 2012, 12(7): 1212-1215.
[10]	YAO Qing, LIU Qing-jie, YANG Bao-jun, CHEN Hong-ming, TANG Jian . An Insect Imaging System to Automate Rice Light-Trap Pest Identification[J]. Journal of Integrative Agriculture, 2012, 12(6): 978-985.
[11]	LI Chuan-xi, SU Ya-ru, WANG Ru-jing, WEI Yuan-yuan, HUANG He. Structured AJAX Data Extraction Based on Agricultural Ontology[J]. Journal of Integrative Agriculture, 2012, 11(5): 784-791.
[12]	LIU Shu-sheng, John Colvin , Paul J De Barro. Species Concepts as Applied to the Whitefly Bemisia tabaci Systematics: How Many Species Are There?[J]. Journal of Integrative Agriculture, 2012, 11(2): 176-186.
[13]	LI Xiao-xi, LI Shao-jian, XUE Xia, Muhammad Z Ahmed, REN Shun-xiang, rew G S Cuthbertson , QIU Bao-li. The Effects of Space Dimension and Temperature on the Cross Mating of Three Cryptic Species of the Bemisia tabaci Complex in China[J]. Journal of Integrative Agriculture, 2012, 11(2): 197-205.
[14]	YE Yun-feng, LI Qi-qin, FU Gang, YUAN Gao-qing, MIAO Jian-hua , LIN Wei. Identification of Antifungal Substance (Iturin A2) Produced by Bacillus subtilis B47 and Its Effect on Southern Corn Leaf Blight[J]. Journal of Integrative Agriculture, 2012, 12(1): 90-99.
[15]	ZHANG Jun-ping, HOU Xi-lin, YU Tian, LI Ying, DONG Hai-yan. Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction[J]. Journal of Integrative Agriculture, 2012, 12(1): 151-158.

The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis

The Extraction, Isolation and Identification of Exudates from the Roots of Flaveria bidentis

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics