Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China

doi:10.1016/S1671-2927(00)8665

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (8): 1347-1353.DOI: 10.1016/S1671-2927(00)8665

Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China

ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam, LI Xiang-rui

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China

收稿日期:2011-03-29 出版日期:2012-08-01 发布日期:2012-09-09
通讯作者: Correspondence LI Xiang-rui, Tel: +86-25-84399000, Fax: +86-25-84399000, E-mail:Lixiangrui@njau.edu.cn
作者简介:ZHAO Guang-wei, Mobile: 18936036162, E-mail: 2009207019@njau.edu.cn
基金资助:
This work was supported by the Special Fund for Public Welfare Industry of Chinese Ministry of Agriculture (200903036-04).

Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China

ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam, LI Xiang-rui

College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095, P.R.China

Received:2011-03-29 Online:2012-08-01 Published:2012-09-09
Contact: Correspondence LI Xiang-rui, Tel: +86-25-84399000, Fax: +86-25-84399000, E-mail:Lixiangrui@njau.edu.cn
About author:ZHAO Guang-wei, Mobile: 18936036162, E-mail: 2009207019@njau.edu.cn
Supported by:
This work was supported by the Special Fund for Public Welfare Industry of Chinese Ministry of Agriculture (200903036-04).

摘要/Abstract

摘要： One strain of Toxoplasma gondii was successfully isolated from chickens in China by bioassay in mice. Antibodies and circulating antigens of T. gondii were assayed by the ELISA kits in 100 free range chickens from a rural area surrounding Funing, China. Fifty-three chickens were antibody-positive and 21 chickens were antigen positive. Hearts, brains, spleens, lungs, livers, and kidneys of 21 antibody or antigen-positive chickens were bioassayed in mice. One strain of T. gondii was isolated from 1 of 21 (4.76%) chickens. The isolated T. gondii killed all of the inoculated mice. Genotyping of this isolate using polymorphisms at the loci 5´-SAG2, 3´-SAG2, SAG3, cB21-4, L358, BTUB, and GRA6 revealed that it was Type I. These indicated that it was virulent for mice. This is the first report of isolation of T. gondii from chickens in China.

关键词: Toxoplasma gondii, chickens, China, isolation

Abstract: One strain of Toxoplasma gondii was successfully isolated from chickens in China by bioassay in mice. Antibodies and circulating antigens of T. gondii were assayed by the ELISA kits in 100 free range chickens from a rural area surrounding Funing, China. Fifty-three chickens were antibody-positive and 21 chickens were antigen positive. Hearts, brains, spleens, lungs, livers, and kidneys of 21 antibody or antigen-positive chickens were bioassayed in mice. One strain of T. gondii was isolated from 1 of 21 (4.76%) chickens. The isolated T. gondii killed all of the inoculated mice. Genotyping of this isolate using polymorphisms at the loci 5´-SAG2, 3´-SAG2, SAG3, cB21-4, L358, BTUB, and GRA6 revealed that it was Type I. These indicated that it was virulent for mice. This is the first report of isolation of T. gondii from chickens in China.

Key words: Toxoplasma gondii, chickens, China, isolation

ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam, LI Xiang-rui. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China[J]. Journal of Integrative Agriculture, 2012, 12(8): 1347-1353.

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Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China

Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China

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