Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (7): 1212-1215.DOI: 10.1016/S1671-2927(00)8649

• 论文 • 上一篇    

A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue

 HU Zhi-yong, ZHAN Gao-miao, WANG Han-zhong,  HUA Wei   

  1. Key Laboratory for Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan 430062, P.R.China
  • 收稿日期:2011-01-09 出版日期:2012-07-01 发布日期:2012-07-27
  • 通讯作者: HUA Wei, Tel: +86-27-86711806, E-mail: huawei@oilcrops.cn
  • 作者简介:HU Zhi-yong, Tel: +86-27-86712076; E-mail: oilhzy@yahoo.com.cn;
  • 基金资助:

    This work was supported by the Ministry of Agriculture of China Genetically Modified Organisms Breeding Major Projects (2009ZX08009-018B).

A Simple Method for Isolating Chloroplast DNA and Mitochondria DNA from the Same Rapeseed Green Leaf Tissue

 HU Zhi-yong, ZHAN Gao-miao, WANG Han-zhong,  HUA Wei   

  1. Key Laboratory for Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture/Oil Crops Research Institute, Chinese Academy of Agriculture Sciences, Wuhan 430062, P.R.China
  • Received:2011-01-09 Online:2012-07-01 Published:2012-07-27
  • Contact: HUA Wei, Tel: +86-27-86711806, E-mail: huawei@oilcrops.cn
  • About author:HU Zhi-yong, Tel: +86-27-86712076; E-mail: oilhzy@yahoo.com.cn;
  • Supported by:

    This work was supported by the Ministry of Agriculture of China Genetically Modified Organisms Breeding Major Projects (2009ZX08009-018B).

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摘要： In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.

关键词: chloroplast DNA, mitochondrial DNA, organelle isolation, rapeseed

Abstract: In the study, we present a fast, simple and inexpensive protocol for isolating chloroplast and mitochondrial DNA from one rapeseed leaf tissue sample. The chloroplast and mitochondria were separated from the same green leaf tissue by differential centrifugations. The protocol is the first report that isolates plant chloroplast DNA (cpDNA) and mitochondrial DNA (mtDNA) from the same sample homogenate. The organelle DNA yield is 2-10 micrograms per gram of tissue; the DNA was fully restrictable and was successfully used for sequencing.

Key words: chloroplast DNA, mitochondrial DNA, organelle isolation, rapeseed