Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction

doi:10.1016/S1671-2927(00)8511

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (1): 151-158.DOI: 10.1016/S1671-2927(00)8511

Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction

ZHANG Jun-ping, HOU Xi-lin, YU Tian, LI Ying, DONG Hai-yan

1.State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agriculture University, Nanjing 210095, P.R.China
2.Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R.China

收稿日期:2010-10-28 出版日期:2012-01-01 发布日期:2012-01-13
通讯作者: Correspondence HOU Xi-lin, Tel: +86-25-84395917, E-mail: hxl@njau.edu.cn
作者简介:ZHANG Jun-ping, E-mail: xj2005zhangjp@126.com
基金资助:
This work was supported by the Public Welfare Industry (Agriculture) Research Program, China (200903018).

Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction

ZHANG Jun-ping, HOU Xi-lin, YU Tian, LI Ying, DONG Hai-yan

1.State Key Laboratory of Crop Genetics & Germplasm Enhancement, Nanjing Agriculture University, Nanjing 210095, P.R.China
2.Key Laboratory of Southern Vegetable Crop Genetic Improvement, Ministry of Agriculture, Nanjing 210095, P.R.China

Received:2010-10-28 Online:2012-01-01 Published:2012-01-13
Contact: Correspondence HOU Xi-lin, Tel: +86-25-84395917, E-mail: hxl@njau.edu.cn
About author:ZHANG Jun-ping, E-mail: xj2005zhangjp@126.com
Supported by:
This work was supported by the Public Welfare Industry (Agriculture) Research Program, China (200903018).

摘要/Abstract

摘要： Supercritical carbon dioxide (SC-CO2) extraction was employed to extract oil from Nigella glandulifera Freyn seed in this study. Response surface methodology (RSM) was applied to evaluate the effects of the process parameters (pressure, temperature, and CO2 flow rate) on oil yield of N. glandulifera seed. A Box-Behnken design was used to optimize the extraction parameters. The analysis of variance indicated that the linear coefficients of pressure and CO2 flow rate, the quadratic term coefficients of pressure and temperature and the interactions between pressure and temperature, as well as temperature and CO2 flow rate, had significant effects on the oil yield (P<0.05). The optimal conditions to obtain the maximum oil yield from N. glandulifera seed were pressure 30.84 MPa, temperature 40.57°C, and CO2 flow rate 22.00 L h-1. Under these optimal conditions, the yield of oil was predicted to be 38.19%. The validation experiment results agreed with the predicted values. The fatty acid composition of N. glandulifera seed oil extracted using SC-CO2 was compared with that of oil obtained by Soxhlet method. The results showed that the fatty acid compositions of oil extracted by the two methods were similar. Identification of oil compounds with gas chromatography-mass spectrometry (GC-MS) showed that the contents of unsaturated fatty acids linoleic acid (48.30%), oleic acid (22.28%) and saturated fatty acids palmitic acid (16.65%), stearic acid (4.17%) were the most abundant fatty acids in seed oil from N. glandulifera.

关键词: supercritical carbon dioxide extraction, Nigella glandulifera Freyn seed oil, response surface methodology, gas chromatography-mass spectrometry, fatty acids

Abstract: Supercritical carbon dioxide (SC-CO2) extraction was employed to extract oil from Nigella glandulifera Freyn seed in this study. Response surface methodology (RSM) was applied to evaluate the effects of the process parameters (pressure, temperature, and CO2 flow rate) on oil yield of N. glandulifera seed. A Box-Behnken design was used to optimize the extraction parameters. The analysis of variance indicated that the linear coefficients of pressure and CO2 flow rate, the quadratic term coefficients of pressure and temperature and the interactions between pressure and temperature, as well as temperature and CO2 flow rate, had significant effects on the oil yield (P<0.05). The optimal conditions to obtain the maximum oil yield from N. glandulifera seed were pressure 30.84 MPa, temperature 40.57°C, and CO2 flow rate 22.00 L h-1. Under these optimal conditions, the yield of oil was predicted to be 38.19%. The validation experiment results agreed with the predicted values. The fatty acid composition of N. glandulifera seed oil extracted using SC-CO2 was compared with that of oil obtained by Soxhlet method. The results showed that the fatty acid compositions of oil extracted by the two methods were similar. Identification of oil compounds with gas chromatography-mass spectrometry (GC-MS) showed that the contents of unsaturated fatty acids linoleic acid (48.30%), oleic acid (22.28%) and saturated fatty acids palmitic acid (16.65%), stearic acid (4.17%) were the most abundant fatty acids in seed oil from N. glandulifera.

Key words: supercritical carbon dioxide extraction, Nigella glandulifera Freyn seed oil, response surface methodology, gas chromatography-mass spectrometry, fatty acids

ZHANG Jun-ping, HOU Xi-lin, YU Tian, LI Ying, DONG Hai-yan. Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction[J]. Journal of Integrative Agriculture, 2012, 12(1): 151-158.

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Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction

Response Surface Optimization of Nigella glandulifera Freyn Seed Oil Yield by Supercritical Carbon Dioxide Extraction

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