Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings

doi:10.1016/S2095-3119(13)60367-6

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (10): 1783-1790.DOI: 10.1016/S2095-3119(13)60367-6

Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings

DONG Yu-xiu, WANG Xiu-feng , CUI Xiu-min

1 Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China
2 College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, P.R.China

收稿日期:2012-09-20 出版日期:2013-10-01 发布日期:2013-10-01
通讯作者: Correspondence CUI Xiu-min, Tel: +86-538-8241546, Mobile: 13905489073, Fax: +86-538-8242250, E-mail: xiumincui@sdau.edu.cn
作者简介:DONG Yu-xiu, E-mail: zwfy1@sdau.edu.cn;
基金资助:
This work was supported partially by the National Natural Science Foundation of China (31201619), Profession Expert Group of Facility Cultivation and Engineering (CARS- 25-D-03) and the Sci-Tech Development Project of Tai’an City, China (32606).

Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings

DONG Yu-xiu, WANG Xiu-feng , CUI Xiu-min

1 Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, P.R.China
2 College of Resources and Environment, Shandong Agricultural University, Tai’an 271018, P.R.China

Received:2012-09-20 Online:2013-10-01 Published:2013-10-01
Contact: Correspondence CUI Xiu-min, Tel: +86-538-8241546, Mobile: 13905489073, Fax: +86-538-8242250, E-mail: xiumincui@sdau.edu.cn
About author:DONG Yu-xiu, E-mail: zwfy1@sdau.edu.cn;
Supported by:
This work was supported partially by the National Natural Science Foundation of China (31201619), Profession Expert Group of Facility Cultivation and Engineering (CARS- 25-D-03) and the Sci-Tech Development Project of Tai’an City, China (32606).

摘要/Abstract

摘要： Nitric oxide (NO), a bioactive signaling molecule, serves as an antioxidant and anti-stress agent under abiotic stress. A hydroponics experiment was conducted to investigate the effects of sodium nitroprusside (SNP), a NO donor, on tomato seedlings exposed to 50 μmol L-1 CuCl2. The results show that copper is primarily stored in the soluble cell sap fraction in the roots, especially after treatment with Cu+SNP treatment, which accounted for 66.2% of the total copper content. The copper concentration gradually decreased from the roots to the leaves. In the leaves, exogenous NO induces the storage of excess copper in the cell walls. Copper stress decreases the proportion of copper integrated with pectates and proteins, but exogenous NO remarkably reverses this trend. The alleviating effect of NO is blocked by hemoglobin. Thus, exogenous NO is likely involved in the regulation of the subcellular copper concentrations and its chemical forms under copper stress. Although exogenous NO inhibited the absorption and transport of excess copper to some extent, the copper accumulation in tomato seedlings significantly increased under copper stress. The use of exogenous NO to enhance copper tolerance in some plants is a promising method for copper remediation.

关键词: tomato seedlings , nitric oxide , copper stress , subcellular distribution , chemical form

Abstract: Nitric oxide (NO), a bioactive signaling molecule, serves as an antioxidant and anti-stress agent under abiotic stress. A hydroponics experiment was conducted to investigate the effects of sodium nitroprusside (SNP), a NO donor, on tomato seedlings exposed to 50 μmol L-1 CuCl2. The results show that copper is primarily stored in the soluble cell sap fraction in the roots, especially after treatment with Cu+SNP treatment, which accounted for 66.2% of the total copper content. The copper concentration gradually decreased from the roots to the leaves. In the leaves, exogenous NO induces the storage of excess copper in the cell walls. Copper stress decreases the proportion of copper integrated with pectates and proteins, but exogenous NO remarkably reverses this trend. The alleviating effect of NO is blocked by hemoglobin. Thus, exogenous NO is likely involved in the regulation of the subcellular copper concentrations and its chemical forms under copper stress. Although exogenous NO inhibited the absorption and transport of excess copper to some extent, the copper accumulation in tomato seedlings significantly increased under copper stress. The use of exogenous NO to enhance copper tolerance in some plants is a promising method for copper remediation.

Key words: tomato seedlings , nitric oxide , copper stress , subcellular distribution , chemical form

DONG Yu-xiu, WANG Xiu-feng , CUI Xiu-min. Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings[J]. Journal of Integrative Agriculture, 2013, 12(10): 1783-1790.

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Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings

Exogenous Nitric Oxide Involved in Subcellular Distribution and Chemical Forms of Cu2+ Under Copper Stress in Tomato Seedlings

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