Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress

doi:10.1016/S2095-3119(13)60268-3

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (5): 815-824.DOI: 10.1016/S2095-3119(13)60268-3

Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress

ZHANG Zi-kun, LI Hua, HE Hong-jun , LIU Shi-qi

1.Dezhou Academy of Agricultural Sciences, Dezhou 253015, P.R.China
2.State Key Laboratory of Crop Biology/Key Laboratory of Horticultural Crop Biology/College of Horticulture Science and Engineering,Shandong Agricultural University, Tai’an 271018, P.R.China

收稿日期:2012-01-10 出版日期:2013-05-01 发布日期:2013-05-01
通讯作者: Correspondence LIU Shi-qi, Tel: +86-538-8246818, E-mail: sqliu@sdau.edu.cn
基金资助:
This work was financially supported by Modern Agricultural Industurl Techenology System Fund of Shandong Province, China (2010-03).

Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress

ZHANG Zi-kun, LI Hua, HE Hong-jun , LIU Shi-qi

1.Dezhou Academy of Agricultural Sciences, Dezhou 253015, P.R.China
2.State Key Laboratory of Crop Biology/Key Laboratory of Horticultural Crop Biology/College of Horticulture Science and Engineering,Shandong Agricultural University, Tai’an 271018, P.R.China

Received:2012-01-10 Online:2013-05-01 Published:2013-05-01
Contact: Correspondence LIU Shi-qi, Tel: +86-538-8246818, E-mail: sqliu@sdau.edu.cn
Supported by:
This work was financially supported by Modern Agricultural Industurl Techenology System Fund of Shandong Province, China (2010-03).

摘要/Abstract

摘要： A greenhouse experiment was carried out to determine plant growth, reactive oxygen species (ROS) metabolism in roots and functions of plasma membrane (PM) and tonoplast in cucumber seedlings (Cucumis sativus L. cv. Xintaimici) treated with 40 μmol L-1 CuSO4·5H2O, which were either ungrafted or grafted onto the rootstock (Cucurbita ficifolia). Cu treatment inhibited growth, induced significant accumulation of H2O2 and led to serious lipid peroxidation in cucumber roots, and the ROS-scavenging enzymes activities in grafted seedlings roots were significantly higher than that of ungrafted plants, thus less accumulation in grafted cucumber roots induced by Cu. As a result, lipid peroxidation in roots decreased. Furthermore, the activities of H+-ATPase, H+-PPase and Ca2+-ATPase in PM and/or tonoplast in grafted cucumber seedlings under Cu stress were obviously higher than that in ungrafted plants, resulting into higher ability in grafted plants to expulse the excess H+, promote the cytoplasm alkalinization, regulate the intracellular Ca2+ concentration and brought the cytoplasma concentration of free Ca2+ to extremely low level under Cu stress.

关键词: copper stress , grafted cucumber seedling , antioxidative enzymes , plasma membrane , tonoplast

Abstract: A greenhouse experiment was carried out to determine plant growth, reactive oxygen species (ROS) metabolism in roots and functions of plasma membrane (PM) and tonoplast in cucumber seedlings (Cucumis sativus L. cv. Xintaimici) treated with 40 μmol L-1 CuSO4·5H2O, which were either ungrafted or grafted onto the rootstock (Cucurbita ficifolia). Cu treatment inhibited growth, induced significant accumulation of H2O2 and led to serious lipid peroxidation in cucumber roots, and the ROS-scavenging enzymes activities in grafted seedlings roots were significantly higher than that of ungrafted plants, thus less accumulation in grafted cucumber roots induced by Cu. As a result, lipid peroxidation in roots decreased. Furthermore, the activities of H+-ATPase, H+-PPase and Ca2+-ATPase in PM and/or tonoplast in grafted cucumber seedlings under Cu stress were obviously higher than that in ungrafted plants, resulting into higher ability in grafted plants to expulse the excess H+, promote the cytoplasm alkalinization, regulate the intracellular Ca2+ concentration and brought the cytoplasma concentration of free Ca2+ to extremely low level under Cu stress.

Key words: copper stress , grafted cucumber seedling , antioxidative enzymes , plasma membrane , tonoplast

ZHANG Zi-kun, LI Hua, HE Hong-jun , LIU Shi-qi. Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress[J]. Journal of Integrative Agriculture, 2013, 12(5): 815-824.

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Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress

Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress

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