Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship

doi:10.1016/S1671-2927(00)8925

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (7): 1138-1151.DOI: 10.1016/S1671-2927(00)8925

Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship

href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((HUANG Li[Author]) AND 1[Journal]) AND year[Order])" target="_blank">HUANG Li, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((ZHAO Xiao-feng[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHAO Xiao-feng, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((ZHU Yi-peng[Author]) AND 1[Journal]) AND year[Order])" target="_blank">ZHU Yi-peng, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((DONG Heng[Author]) AND 1[Journal]) AND year[Order])" target="_blank">DONG Heng, href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((XU Ning-ying [Author]) AND 1[Journal]) AND year[Order])" target="_blank">XU Ning-ying , href="https://www.chinaagrisci.com/Jwk_zgnykxen/CN/article/advancedSearchResult.do?searchSQL=(((CAO Jia-shu[Author]) AND 1[Journal]) AND year[Order])" target="_blank">CAO Jia-shu

1.Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, P.R.China
2.College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, P.R.China
3.Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310058, P.R.China
4.College of Animal Science, Zhejiang University, Hangzhou 310058, P.R.China

收稿日期:2012-09-05 出版日期:2013-07-01 发布日期:2013-07-04
通讯作者: Correspondence CAO Jia-shu, Tel: +86-571-88982188, Fax: +86-571-88982188, E-mail: jshcao@zju.edu.cn
作者简介:HUANG Li, E-mail: lihuang@zju.edu.cn
基金资助:
This work was supported by the Key Sci-Technology Project of Zhejiang Province (2010C12004), the Sci-Technology Project of Zhejiang Province (2009C32026) and the Sci-Technology Project of Jiaxing City, Zhejiang, China (2010AZ1004).

Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship

HUANG Li, ZHAO Xiao-feng, ZHU Yi-peng, DONG Heng, XU Ning-ying , CAO Jia-shu

1.Laboratory of Cell & Molecular Biology, Institute of Vegetable Science, Zhejiang University, Hangzhou 310058, P.R.China
2.College of Life and Environment Sciences, Hangzhou Normal University, Hangzhou 310036, P.R.China
3.Key Laboratory of Horticultural Plant Growth, Development and Quality Improvement, Ministry of Agriculture, Hangzhou 310058, P.R.China
4.College of Animal Science, Zhejiang University, Hangzhou 310058, P.R.China

Received:2012-09-05 Online:2013-07-01 Published:2013-07-04
Contact: Correspondence CAO Jia-shu, Tel: +86-571-88982188, Fax: +86-571-88982188, E-mail: jshcao@zju.edu.cn
About author:HUANG Li, E-mail: lihuang@zju.edu.cn
Supported by:
This work was supported by the Key Sci-Technology Project of Zhejiang Province (2010C12004), the Sci-Technology Project of Zhejiang Province (2009C32026) and the Sci-Technology Project of Jiaxing City, Zhejiang, China (2010AZ1004).

摘要/Abstract

摘要： DNA barcodes have been proposed as a shortcut to provide species identification and as a way to accelerate the discovery of new species. A number of candidate gene regions have been suggested as possible barcodes for animals and plants, but for the identification of recently diverged species and/or varieties with only a few genetic differences it has been reported to be problematic in some cases. This study selected widely cultivated cruciferous vegetables as the primary samples, after failure of discrimination of each species using current DNA barcodes, we performed the fluorescent amplified fragment length polymorphism (F-AFLP) and successfully discriminated each species, subspecies, variety and their cultivar in 74 samples. Then the non-qualitative results obtained from F-AFLP were transformed into two-dimensional barcodes image file of each cultivar via the PDF417 software. This method was also successfully applied to the discrimination of 17 Chinese indigenous pig breeds. The barcode we constructed which greatly reduces the information storage space is genotypes-specific, and can be conveniently decoded into the original data and thereby be conveniently shared and referred to. We believe that it is possible to construct a new data sharing molecular barcode system that could discriminate the subspecies, varieties, cultivars and even individuals with close genetic relationships.

关键词: molecular barcode , molecular marker , Cruciferae , Sus , F-AFLP

Abstract: DNA barcodes have been proposed as a shortcut to provide species identification and as a way to accelerate the discovery of new species. A number of candidate gene regions have been suggested as possible barcodes for animals and plants, but for the identification of recently diverged species and/or varieties with only a few genetic differences it has been reported to be problematic in some cases. This study selected widely cultivated cruciferous vegetables as the primary samples, after failure of discrimination of each species using current DNA barcodes, we performed the fluorescent amplified fragment length polymorphism (F-AFLP) and successfully discriminated each species, subspecies, variety and their cultivar in 74 samples. Then the non-qualitative results obtained from F-AFLP were transformed into two-dimensional barcodes image file of each cultivar via the PDF417 software. This method was also successfully applied to the discrimination of 17 Chinese indigenous pig breeds. The barcode we constructed which greatly reduces the information storage space is genotypes-specific, and can be conveniently decoded into the original data and thereby be conveniently shared and referred to. We believe that it is possible to construct a new data sharing molecular barcode system that could discriminate the subspecies, varieties, cultivars and even individuals with close genetic relationships.

Key words: molecular barcode , molecular marker , Cruciferae , Sus , F-AFLP

HUANG Li, ZHAO Xiao-feng, ZHU Yi-peng, DONG Heng, XU Ning-ying , CAO Jia-shu. Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship[J]. Journal of Integrative Agriculture, 2013, 12(7): 1138-1151.

HUANG Li, ZHAO Xiao-feng, ZHU Yi-peng, DONG Heng, XU Ning-ying , CAO Jia-shu, . Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship[J]. Journal of Integrative Agriculture, 2013, 12(7): 1138-1151.

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Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship

Construction of a New Molecular Barcode for Discriminating Plants and Animals with a Close Genetic Relationship

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