Flor Revisited (Again): eQTL and Mutational Analysis of NB-LRR Mediated Immunity to Powdery Mildew in Barley

doi:10.1016/S2095-3119(13)60651-6

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (2): 237-243.DOI: 10.1016/S2095-3119(13)60651-6

Flor Revisited (Again): eQTL and Mutational Analysis of NB-LRR Mediated Immunity to Powdery Mildew in Barley

Roger Wise, Priyanka Surana, Greg Fuerst, Ruo Xu, Divya Mistry, Julie Dickerson

1.Corn Insects and Crop Genetics Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Iowa State University, Ames,
Iowa 50011, USA
2.Department of Plant Pathology & Microbiology, Center for Plant Responses to Environmental Stresses, Iowa State University, Ames, Iowa
50011, USA
3.Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, Iowa 50011, USA
4.Department of Statistics, Iowa State University, Ames, Iowa 50011, USA
5.Department of Electrical and Computer Engineering, Iowa State University, Ames, Iowa 50011, USA

收稿日期:2013-07-20 出版日期:2014-02-01 发布日期:2014-02-06
通讯作者: Roger Wise, E-mail: rpwise@iastate.edu
作者简介:Roger Wise, E-mail: rpwise@iastate.edu
基金资助:
Research supported in part by USA National Science Foundation-Plant Genome Program grant (0922746).

Flor Revisited (Again): eQTL and Mutational Analysis of NB-LRR Mediated Immunity to Powdery Mildew in Barley

Roger Wise, Priyanka Surana, Greg Fuerst, Ruo Xu, Divya Mistry, Julie Dickerson

1.Corn Insects and Crop Genetics Research Unit, U.S. Department of Agriculture-Agricultural Research Service, Iowa State University, Ames,
Iowa 50011, USA
2.Department of Plant Pathology & Microbiology, Center for Plant Responses to Environmental Stresses, Iowa State University, Ames, Iowa
50011, USA
3.Bioinformatics and Computational Biology Graduate Program, Iowa State University, Ames, Iowa 50011, USA
4.Department of Statistics, Iowa State University, Ames, Iowa 50011, USA
5.Department of Electrical and Computer Engineering, Iowa State University, Ames, Iowa 50011, USA

Received:2013-07-20 Online:2014-02-01 Published:2014-02-06
Contact: Roger Wise, E-mail: rpwise@iastate.edu
About author:Roger Wise, E-mail: rpwise@iastate.edu
Supported by:
Research supported in part by USA National Science Foundation-Plant Genome Program grant (0922746).

摘要/Abstract

摘要： Genes encoding early signaling events in pathogen defense often are identified only by their phenotype. Such genes involved in barley-powdery mildew interactions include Mla, specifying race-specific resistance; Rar1 (Required for Mla12-specified resistance1), and Rom1 (Restoration of Mla-specified resistance1). The HSP90-SGT1-RAR1 complex appears to function as chaperone in MLA-specified resistance, however, much remains to be discovered regarding the precise signaling underlying plant immunity. Genetic analyses of fast-neutron mutants derived from CI 16151 (Mla6) uncovered a novel locus, designated Rar3 (Required for Mla6-specified resistance3). Rar3 segregates independent of Mla6 and Rar1, and rar3 mutants are susceptible to Blumeria graminis f. sp. hordei (Bgh) isolate 5874 (AVRa6), whereas, wild-type progenitor plants are resistant. Comparative expression analyses of the rar3 mutant vs. its wild-type progenitor were conducted via Barley1 GeneChip and GAIIx paired-end RNA-Seq. Whereas Rar1 affects transcription of relatively few genes; Rar3 appears to influence thousands, notably in genes controlling ATP binding, catalytic activity, transcription, and phosphorylation; possibly membrane bound or in the nucleus. eQTL analysis of a segregating doubled haploid population identified over two-thousand genes as being regulated by Mla (q value/FDR=0.00001), a subset of which are significant in Rar3 interactions. The intersection of datasets derived from mla-loss-of-function mutants, Mla-associated eQTL, and rar3-mediated transcriptome reprogramming are narrowing the focus on essential genes required for Mla-specified immunity.

关键词: eQTL , transcript profiling , immunity , resistance signaling , barley , Blumeria graminis

Abstract: Genes encoding early signaling events in pathogen defense often are identified only by their phenotype. Such genes involved in barley-powdery mildew interactions include Mla, specifying race-specific resistance; Rar1 (Required for Mla12-specified resistance1), and Rom1 (Restoration of Mla-specified resistance1). The HSP90-SGT1-RAR1 complex appears to function as chaperone in MLA-specified resistance, however, much remains to be discovered regarding the precise signaling underlying plant immunity. Genetic analyses of fast-neutron mutants derived from CI 16151 (Mla6) uncovered a novel locus, designated Rar3 (Required for Mla6-specified resistance3). Rar3 segregates independent of Mla6 and Rar1, and rar3 mutants are susceptible to Blumeria graminis f. sp. hordei (Bgh) isolate 5874 (AVRa6), whereas, wild-type progenitor plants are resistant. Comparative expression analyses of the rar3 mutant vs. its wild-type progenitor were conducted via Barley1 GeneChip and GAIIx paired-end RNA-Seq. Whereas Rar1 affects transcription of relatively few genes; Rar3 appears to influence thousands, notably in genes controlling ATP binding, catalytic activity, transcription, and phosphorylation; possibly membrane bound or in the nucleus. eQTL analysis of a segregating doubled haploid population identified over two-thousand genes as being regulated by Mla (q value/FDR=0.00001), a subset of which are significant in Rar3 interactions. The intersection of datasets derived from mla-loss-of-function mutants, Mla-associated eQTL, and rar3-mediated transcriptome reprogramming are narrowing the focus on essential genes required for Mla-specified immunity.

Key words: eQTL , transcript profiling , immunity , resistance signaling , barley , Blumeria graminis

Roger Wise, Priyanka Surana, Greg Fuerst, Ruo Xu, Divya Mistry, Julie Dickerson. Flor Revisited (Again): eQTL and Mutational Analysis of NB-LRR Mediated Immunity to Powdery Mildew in Barley[J]. Journal of Integrative Agriculture, 2014, 13(2): 237-243.

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Flor Revisited (Again): eQTL and Mutational Analysis of NB-LRR Mediated Immunity to Powdery Mildew in Barley

Flor Revisited (Again): eQTL and Mutational Analysis of NB-LRR Mediated Immunity to Powdery Mildew in Barley

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