Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR

doi:10.1016/S1671-2927(11)60137-5

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (9): 1445-1451.DOI: 10.1016/S1671-2927(11)60137-5

Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR

FU Yan-feng, FU Jin-luan, YANG Lu, TIAN Ming-ming, CHEN Wen-cheng , WANG Ai-guo

College of Animal Science and Technology, China Agricultural University/Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture

收稿日期:2010-07-21 出版日期:2011-09-01 发布日期:2011-09-09
通讯作者: Correspondence FU Jin-luan, Professor, Tel/Fax: +86-10-62733743, E-mail: fujinlian@126.com; WANG Ai-guo,Professor, Tel/Fax: +86-10-62733743, E-mail: agwang@cau.edu.cn
作者简介:FU Yan-feng, Ph D, E-mail: fuyanfeng@gmail.com
基金资助:
This research was supported by the National NaturalScience Foundation of China (30771540) and the NationalHigh-Technology Research Development Programof China (2007AA10Z166). The authors greatly appreciateBeijing Huadu Swine Breeding Company, Ltd.,China, for assistance in collecting samples.

Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR

FU Yan-feng, FU Jin-luan, YANG Lu, TIAN Ming-ming, CHEN Wen-cheng , WANG Ai-guo

College of Animal Science and Technology, China Agricultural University/Key Laboratory of Animal Genetics and Breeding, Ministry of Agriculture

Received:2010-07-21 Online:2011-09-01 Published:2011-09-09
Contact: Correspondence FU Jin-luan, Professor, Tel/Fax: +86-10-62733743, E-mail: fujinlian@126.com; WANG Ai-guo,Professor, Tel/Fax: +86-10-62733743, E-mail: agwang@cau.edu.cn
About author:FU Yan-feng, Ph D, E-mail: fuyanfeng@gmail.com
Supported by:
This research was supported by the National NaturalScience Foundation of China (30771540) and the NationalHigh-Technology Research Development Programof China (2007AA10Z166). The authors greatly appreciateBeijing Huadu Swine Breeding Company, Ltd.,China, for assistance in collecting samples.

摘要/Abstract

摘要： Erythropoietin-producing hepatocellular receptor and its membrane-bound ligands (Eph-Ephrin) system could regulatesome mammalian blastocyst attachment and spreading. In order to investigate the involvement of the Eph-Ephrin systemin swine embryo attachment, mRNA expression of Eph-Ephrin molecules in endometrium was examined by real-time RTPCRduring embryo implantation in pigs. The results indicated that mRNA expressions of Eph A5, A7 and Ephrin A5 allcontinually increased from pregnancy day 13 to 24. Ephrin A3 mRNA expression significantly increased from day 13 to 18and decreased from day 18 to 24, and the expression was the lowest on pregnancy day 13 and the highest on day 18.However, Ephrin A4 mRNA expression was the lowest on pregnancy day 18 and the highest on day 24, and the expressiondecreased from day 13 to 18 and increased from day 18 to 24. Furthermore, mRNA expressions of Eph A5 and A7 were bothfound in other tissues, such as brain, muscle, intestine, stomach, etc. These findings suggest that the Eph-Ephrin systemmay play an important role in regulating the contact between blastocysts and endometrium during swine embryoimplantation.

关键词:

Eph-Ephrin, real-time, RT-PCR, endometrium, implantation, pig

Abstract: Erythropoietin-producing hepatocellular receptor and its membrane-bound ligands (Eph-Ephrin) system could regulatesome mammalian blastocyst attachment and spreading. In order to investigate the involvement of the Eph-Ephrin systemin swine embryo attachment, mRNA expression of Eph-Ephrin molecules in endometrium was examined by real-time RTPCRduring embryo implantation in pigs. The results indicated that mRNA expressions of Eph A5, A7 and Ephrin A5 allcontinually increased from pregnancy day 13 to 24. Ephrin A3 mRNA expression significantly increased from day 13 to 18and decreased from day 18 to 24, and the expression was the lowest on pregnancy day 13 and the highest on day 18.However, Ephrin A4 mRNA expression was the lowest on pregnancy day 18 and the highest on day 24, and the expressiondecreased from day 13 to 18 and increased from day 18 to 24. Furthermore, mRNA expressions of Eph A5 and A7 were bothfound in other tissues, such as brain, muscle, intestine, stomach, etc. These findings suggest that the Eph-Ephrin systemmay play an important role in regulating the contact between blastocysts and endometrium during swine embryoimplantation.

Key words:

Eph-Ephrin, real-time, RT-PCR, endometrium, implantation, pig

FU Yan-feng, FU Jin-luan, YANG Lu, TIAN Ming-ming, CHEN Wen-cheng , WANG Ai-guo. Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR[J]. Journal of Integrative Agriculture, 2011, 10(9): 1445-1451.

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Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR

Expression of Eph-Ephrin A Molecules in Endometrium During Swine Embryo Implantation Examined Using Real-Time RT-PCR

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