Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress

doi:10.1016/S2095-3119(13)60696-6

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (11): 2407-2415.DOI: 10.1016/S2095-3119(13)60696-6

Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress

WENG Chang-mei, LU Jun-xing, WAN Hua-fang, WANG Shu-wen, WANG Zhen, LU Kun, LIANG Ying

1、College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, P.R.China
2、Chongqing Rapeseed Engineering & Technology Research Center, Chongqing 400715, P.R.China
3、Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, P.R.China

收稿日期:2013-10-28 出版日期:2014-11-06 发布日期:2014-11-14
通讯作者: LIANG Ying, E-mail: yliang@swu.edu.cn
作者简介:WENG Chang-mei, E-mail: changmei1988@sina.com
基金资助:
This study was supported by the National Natural Science Foundation of China (31271756, 31101175) and the National “111” Project of China’s Higher Education (B12006).

Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress

WENG Chang-mei, LU Jun-xing, WAN Hua-fang, WANG Shu-wen, WANG Zhen, LU Kun, LIANG Ying

1、College of Agronomy and Biotechnology, Southwest University, Chongqing 400715, P.R.China
2、Chongqing Rapeseed Engineering & Technology Research Center, Chongqing 400715, P.R.China
3、Engineering Research Center of South Upland Agriculture, Ministry of Education, Chongqing 400715, P.R.China

Received:2013-10-28 Online:2014-11-06 Published:2014-11-14
Contact: LIANG Ying, E-mail: yliang@swu.edu.cn
About author:WENG Chang-mei, E-mail: changmei1988@sina.com
Supported by:
This study was supported by the National Natural Science Foundation of China (31271756, 31101175) and the National “111” Project of China’s Higher Education (B12006).

摘要/Abstract

摘要： Mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases widely conserved in all eukaryotes and involved in responses to biotic and abiotic stresses. In this study, two over-expressing BnMAPK1 oilseed rape lines, ov3 and ov11, were used to study the drought-resistant mechanism of BnMAPK1 under natural drought and simulation drought through spraying 10% PEG 8000 in seedlings. Zhongyou 821 (WT) was used as control. Compared with wild type, transgenic seedlings had higher leaf water content, higher root activity, slightly higher peroxidase (POD) and superoxide dismutase (SOD) activity, higher proline content and lower malondialdehyde (MDA) content. The expression of drought-resistant related genes, including P5CSB, PLC, LEA4 and SCE1, have been up-regulated in some degree and the expressed time of transgenic lines were earlier than that of wild type. These results suggested that over-expression of BnMAPK1 can enhance the resistance to drought in oilseed rape (Brassica napus).

关键词: MAPK , Brassica napus , transgenic , drought-stress , qRT-PCR

Abstract: Mitogen-activated protein kinases (MAPKs) are a family of Ser/Thr protein kinases widely conserved in all eukaryotes and involved in responses to biotic and abiotic stresses. In this study, two over-expressing BnMAPK1 oilseed rape lines, ov3 and ov11, were used to study the drought-resistant mechanism of BnMAPK1 under natural drought and simulation drought through spraying 10% PEG 8000 in seedlings. Zhongyou 821 (WT) was used as control. Compared with wild type, transgenic seedlings had higher leaf water content, higher root activity, slightly higher peroxidase (POD) and superoxide dismutase (SOD) activity, higher proline content and lower malondialdehyde (MDA) content. The expression of drought-resistant related genes, including P5CSB, PLC, LEA4 and SCE1, have been up-regulated in some degree and the expressed time of transgenic lines were earlier than that of wild type. These results suggested that over-expression of BnMAPK1 can enhance the resistance to drought in oilseed rape (Brassica napus).

Key words: MAPK , Brassica napus , transgenic , drought-stress , qRT-PCR

WENG Chang-mei, LU Jun-xing, WAN Hua-fang, WANG Shu-wen, WANG Zhen, LU Kun, LIANG Ying. Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress[J]. Journal of Integrative Agriculture, 2014, 13(11): 2407-2415.

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Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress

Over-Expression of BnMAPK1 in Brassica napus Enhances Tolerance to Drought Stress

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