Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (2): 229-238.DOI: 10.1016/S2095-3119(13)60222-1

• 论文 • 上一篇    下一篇

Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize

 YU Jiang-hua, ZHAO Yan-xin, QIN Ya-ting, YUE Bing, ZHENG Yong-lian , XIAO Hai-lin   

  1. National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R.China
  • 收稿日期:2012-04-19 出版日期:2013-02-01 发布日期:2013-02-06
  • 通讯作者: Correspondence XIAO Hai-lin, Mobile: 18627050215, E-mail: hailinxiao@gmail.com
  • 作者简介:YU Jiang-hua, Tel: +86-27-87282689, E-mail: jianghuayu@gmail.com
  • 基金资助:

    This study was financially supported by the National Natural Science Foundation of China (31171565).

Discovery of MicroRNAs Associated with the S Type Cytoplasmic Male Sterility in Maize

 YU Jiang-hua, ZHAO Yan-xin, QIN Ya-ting, YUE Bing, ZHENG Yong-lian , XIAO Hai-lin   

  1. National Key Laboratory of Crop Genetic Improvement/Huazhong Agricultural University, Wuhan 430070, P.R.China
  • Received:2012-04-19 Online:2013-02-01 Published:2013-02-06
  • Contact: Correspondence XIAO Hai-lin, Mobile: 18627050215, E-mail: hailinxiao@gmail.com
  • About author:YU Jiang-hua, Tel: +86-27-87282689, E-mail: jianghuayu@gmail.com
  • Supported by:

    This study was financially supported by the National Natural Science Foundation of China (31171565).

摘要: MicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value<0.01 and 0.011.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.

关键词: CMS-S , microRNAs , real-time PCR , Solexa sequencing , target gene , maize

Abstract: MicroRNAs (miRNAs) are endogenous small RNAs that play important regulatory roles in the growth and development processes of plants and animals. In this study, we examined the expression profiles of pollen miRNAs from a maize S type cytoplasmic male sterile line and its fertility restored line. In total, 100 known miRNAs belonging to 20 families and 81 novel miRNAs belonging to 44 families were identified. Two and seven known miRNAs had significant expression difference between the two lines at the level of P-value<0.01 and 0.011.5 fold expression difference were verified by stem-loop RT-qPCR. Gene Ontology analysis of miRNA target genes revealed that these genes mainly participated in the transcriptional regulation processes.

Key words: CMS-S , microRNAs , real-time PCR , Solexa sequencing , target gene , maize