Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae

doi:10.1016/S2095-3119(13)60503-1

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (12): 2189-2196.DOI: 10.1016/S2095-3119(13)60503-1

Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae

LI Xue-song, XU Fei, WANG Hong-kai , LIN Fu-cheng

1.State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, P.R.China
2.Bioinformatics Lab, Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China

收稿日期:2013-01-04 出版日期:2013-12-01 发布日期:2013-12-17
通讯作者: WANG Hong-kai, E-mail: hkwang@zju.edu.cn
基金资助:
This study was supported by the Natural Science Foundation of Zhejiang Province, China (Y3110028 and LQ12C14003).

Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae

LI Xue-song, XU Fei, WANG Hong-kai , LIN Fu-cheng

1.State Key Laboratory for Rice Biology, Biotechnology Institute, Zhejiang University, Hangzhou 310058, P.R.China
2.Bioinformatics Lab, Institute of Digital Agriculture, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China

Received:2013-01-04 Online:2013-12-01 Published:2013-12-17
Contact: WANG Hong-kai, E-mail: hkwang@zju.edu.cn
Supported by:
This study was supported by the Natural Science Foundation of Zhejiang Province, China (Y3110028 and LQ12C14003).

摘要/Abstract

摘要： The rice blast, caused by fungus Magnaporthe oryzae, is a major constraint to the world food security. Hyphal growth is the foundation of fungal development and proliferation of fungi. To investigate genes involved in hyphal growth of this fungus, digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity, with its related wildtype strain. 416 genes were detected as differential expression, 178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant. Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuolerelated processes. The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M. oryzae pathogenesis.

关键词: differential expressed gene , qRT-PCR , gene ontology , hyphal growth , vacuole differential expressed gene , qRT-PCR , gene ontology , hyphal growth , vacuole

Abstract: The rice blast, caused by fungus Magnaporthe oryzae, is a major constraint to the world food security. Hyphal growth is the foundation of fungal development and proliferation of fungi. To investigate genes involved in hyphal growth of this fungus, digital gene expression tag profiling was used to compare a previously generated temperature-sensitive mutant which defect at hyphae growth and reduction on pathogenicity, with its related wildtype strain. 416 genes were detected as differential expression, 178 of which were specifically expressed in Guy-11 but down-regulated expression in the mutant. Functional classification analysis revealed the phenotype mutation may be mainly caused by a defection in translational and vacuolerelated processes. The results and the protocol used will improve our knowledge on morphogenesis and promote the further study on M. oryzae pathogenesis.

Key words: differential expressed gene , qRT-PCR , gene ontology , hyphal growth , vacuole differential expressed gene , qRT-PCR , gene ontology , hyphal growth , vacuole

LI Xue-song, XU Fei, WANG Hong-kai , LIN Fu-cheng. Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae[J]. Journal of Integrative Agriculture, 2013, 12(12): 2189-2196.

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Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae

Gene Expression Profiling Related to Hyphal Growth in a Temperature- Sensitive Mutant of Magnaporthe oryzae

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