Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato

doi:10.1016/S2095-3119(14)60973-4

Journal of Integrative Agriculture ›› 2016, Vol. 15 ›› Issue (2): 271-281.DOI: 10.1016/S2095-3119(14)60973-4

Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato

WANG Fei-bing, ZHAI Hong, AN Yan-yan, SI Zeng-zhi, HE Shao-zhen, LIU Qing-chang

Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing 100193, P.R.China

收稿日期:2015-02-06 出版日期:2016-02-05 发布日期:2016-02-01
通讯作者: LIU Qing-chang, Tel/Fax: +86-10-62733710,E-mail: liuqc@cau.edu.cn
作者简介:* These authors contributed equally to this study.
基金资助:
This work was supported by the China Agriculture Research System (CARS-11, Sweetpotato).

Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato

WANG Fei-bing, ZHAI Hong, AN Yan-yan, SI Zeng-zhi, HE Shao-zhen, LIU Qing-chang

Beijing Key Laboratory of Crop Genetic Improvement/Laboratory of Crop Heterosis and Utilization, Ministry of Education, China Agricultural University, Beijing 100193, P.R.China

Received:2015-02-06 Online:2016-02-05 Published:2016-02-01
Contact: LIU Qing-chang, Tel/Fax: +86-10-62733710,E-mail: liuqc@cau.edu.cn
About author:* These authors contributed equally to this study.
Supported by:
This work was supported by the China Agriculture Research System (CARS-11, Sweetpotato).

摘要/Abstract

摘要： Myo-inositol-1-phosphate synthase (MIPS) is a key rate limiting enzyme in the de novo biosynthesis of myo-inositol in plants. In the present study, the IbMIPS1 gene was introduced into sweetpotato cultivar Xushu 18 and the transgenic plants exhibited significantly enhanced salt tolerance compared with the wild-type (WT). Overexpression of IbMIPS1 up-regulated the salt stress responsive genes, including myo-inositol monophosphatase (MIPP), pyrroline-5-carboxylate synthase (P5CS), pyrroline-5-carboxylate reductase (P5CR), psbA, phosphoribulokinase (PRK), and superoxide dismutase (SOD) genes, under salt stress. Inositol and proline content, SOD and photosynthesis activities were significantly increased, whereas malonaldehyde (MDA) and H2O2 contents were significantly decreased in the transgenic plants. These findings suggest that the IbMIPS1 gene may enhance salt tolerance of sweetpotato by regulating the expression of salt stress responsive genes, increasing the content of inositol and proline and enhancing the activity of photosynthesis.

关键词: IbMIPS1 , overexpression , salt tolerance , sweetpotato

Abstract: Myo-inositol-1-phosphate synthase (MIPS) is a key rate limiting enzyme in the de novo biosynthesis of myo-inositol in plants. In the present study, the IbMIPS1 gene was introduced into sweetpotato cultivar Xushu 18 and the transgenic plants exhibited significantly enhanced salt tolerance compared with the wild-type (WT). Overexpression of IbMIPS1 up-regulated the salt stress responsive genes, including myo-inositol monophosphatase (MIPP), pyrroline-5-carboxylate synthase (P5CS), pyrroline-5-carboxylate reductase (P5CR), psbA, phosphoribulokinase (PRK), and superoxide dismutase (SOD) genes, under salt stress. Inositol and proline content, SOD and photosynthesis activities were significantly increased, whereas malonaldehyde (MDA) and H2O2 contents were significantly decreased in the transgenic plants. These findings suggest that the IbMIPS1 gene may enhance salt tolerance of sweetpotato by regulating the expression of salt stress responsive genes, increasing the content of inositol and proline and enhancing the activity of photosynthesis.

Key words: IbMIPS1 , overexpression , salt tolerance , sweetpotato

WANG Fei-bing, ZHAI Hong, AN Yan-yan, SI Zeng-zhi, HE Shao-zhen, LIU Qing-chang. Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato[J]. Journal of Integrative Agriculture, 2016, 15(2): 271-281.

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Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato

Overexpression of IbMIPS1 gene enhances salt tolerance in transgenic sweetpotato

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