Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

doi:10.1016/S2095-3119(14)60943-6

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (9): 1808-1815.DOI: 10.1016/S2095-3119(14)60943-6

Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

ZHANG Wen-wu, KONG Li-na, ZHANG De-xiang, JI Cong-liang, ZHANG Xi-quan, LUO Qing-bin

1、College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China
2、Key Laboary of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture/South China Agricultural University,Guangzhou 510642, P.R.China
3、Guangdong Enterprise Laboary of Healthy Animal Husbandry and Environment Control, South China Agricultural University,uangzhou 510642, P.R.China
4、Guangdong Wens Food Group Limited Company, Xinxing 527439, P.R.China

收稿日期:2014-09-29 出版日期:2015-09-03 发布日期:2015-09-05
通讯作者: LUO Qing-bin, Tel: +86-20-85285702,E-mail: qbluo@scau.edu.cn
作者简介:ZHANG Wen-wu, E-mail: 125714992@qq.com;
基金资助:
This research was supported the National Key Technology R&D Program of China (2014BAD08B08) and the Key Technology Research and Development Program of Guangdong Emerging Strategic Industries, China (2012A020800005).

Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

ZHANG Wen-wu, KONG Li-na, ZHANG De-xiang, JI Cong-liang, ZHANG Xi-quan, LUO Qing-bin

1、College of Animal Science, South China Agricultural University, Guangzhou 510642, P.R.China
2、Key Laboary of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture/South China Agricultural University,Guangzhou 510642, P.R.China
3、Guangdong Enterprise Laboary of Healthy Animal Husbandry and Environment Control, South China Agricultural University,uangzhou 510642, P.R.China
4、Guangdong Wens Food Group Limited Company, Xinxing 527439, P.R.China

Received:2014-09-29 Online:2015-09-03 Published:2015-09-05
Contact: LUO Qing-bin, Tel: +86-20-85285702,E-mail: qbluo@scau.edu.cn
About author:ZHANG Wen-wu, E-mail: 125714992@qq.com;
Supported by:
This research was supported the National Key Technology R&D Program of China (2014BAD08B08) and the Key Technology Research and Development Program of Guangdong Emerging Strategic Industries, China (2012A020800005).

摘要/Abstract

摘要： Heat stress is one of the main factors that influence poultry production. Heat shock proteins (HSPs) are known to affect heat tolerance. The formation of HSPs is regulated by heat shock transcription factor 3 (HSF3) in chicken. A DNA pool was established for identifying single nucleotide polymorphisms (SNPs) of the chicken HSF3, and 13 SNPs were detected. The bioinformatic analysis showed that 8 SNPs had the capacity to alter the transcription activity of HSF3. The dual luciferase report gene assay showed that there was a significant difference (P<0.01) in the Firefly luciferase/Renilla luciferase ratio (F/R) of C.–1 703 A>G (S1) and C.–1 388 A>G (S4) sites at the 5´-untranslated region (UTR) of chicken HSF3. The electrophoretic mobility shift assay showed that the S4 site was a transcription binding factor. The analysis of the association of the S1 and S4 sites with heat tolerance index revealed that the S4 site was significantly correlated with the CD3+ T cell, corticosterone, and T3 levels in Lingshan chickens and with the heterophil/lymphocyte value in White Recessive Rock. These results showed that the S4 site at the 5´ UTR of chicken HSF3 might have an impact on heat tolerance in summer and could be used as a potential marker for the selection of chicken with heat tolerance in the future.

关键词: chicken , heat shock factor 3 , dual luciferase report gene , heat tolerance

Abstract: Heat stress is one of the main factors that influence poultry production. Heat shock proteins (HSPs) are known to affect heat tolerance. The formation of HSPs is regulated by heat shock transcription factor 3 (HSF3) in chicken. A DNA pool was established for identifying single nucleotide polymorphisms (SNPs) of the chicken HSF3, and 13 SNPs were detected. The bioinformatic analysis showed that 8 SNPs had the capacity to alter the transcription activity of HSF3. The dual luciferase report gene assay showed that there was a significant difference (P<0.01) in the Firefly luciferase/Renilla luciferase ratio (F/R) of C.–1 703 A>G (S1) and C.–1 388 A>G (S4) sites at the 5´-untranslated region (UTR) of chicken HSF3. The electrophoretic mobility shift assay showed that the S4 site was a transcription binding factor. The analysis of the association of the S1 and S4 sites with heat tolerance index revealed that the S4 site was significantly correlated with the CD3+ T cell, corticosterone, and T3 levels in Lingshan chickens and with the heterophil/lymphocyte value in White Recessive Rock. These results showed that the S4 site at the 5´ UTR of chicken HSF3 might have an impact on heat tolerance in summer and could be used as a potential marker for the selection of chicken with heat tolerance in the future.

Key words: chicken , heat shock factor 3 , dual luciferase report gene , heat tolerance

ZHANG Wen-wu, KONG Li-na, ZHANG De-xiang, JI Cong-liang, ZHANG Xi-quan, LUO Qing-bin. Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance[J]. Journal of Integrative Agriculture, 2015, 14(9): 1808-1815.

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Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance

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