Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.)

doi:10.1016/S1671-2927(00)8553

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (3): 359-367.DOI: 10.1016/S1671-2927(00)8553

Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.)

CHENG Li-rui, Veronica Uzokwe, WANG Yun, ZHU Linghua

1.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural
Sciences, Beijing 100081, P.R.China
2.Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
3.International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

收稿日期:2011-02-10 出版日期:2012-03-01 发布日期:2012-03-11
通讯作者: Correspondence XU Jian-long, Tel: +86-10-82105854, Fax: +86-10-82108559, E-mail: xujlcaas@yahoo.com.cn
基金资助:
The work was funded by the Projectof the 863 Program (2010AA101803) and the 948 Programof China (2006-G51 and 2010-G2B).

Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.)

CHENG Li-rui, Veronica Uzokwe, WANG Yun, ZHU Linghua

1.National Key Facility for Crop Gene Resources and Genetic Improvement/Institute of Crop Sciences, Chinese Academy of Agricultural
Sciences, Beijing 100081, P.R.China
2.Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, P.R.China
3.International Rice Research Institute, DAPO Box 7777, Metro Manila, Philippines

Received:2011-02-10 Online:2012-03-01 Published:2012-03-11
Contact: Correspondence XU Jian-long, Tel: +86-10-82105854, Fax: +86-10-82108559, E-mail: xujlcaas@yahoo.com.cn
Supported by:
The work was funded by the Projectof the 863 Program (2010AA101803) and the 948 Programof China (2006-G51 and 2010-G2B).

摘要/Abstract

摘要： A set of 240 introgression lines derived from the advanced backcross population of a cross between a japonica cultivar,Xiushui 09, and an indica breeding line, IR2061, was developed to dissect QTLs affecting cold tolerance (CT) at seedlingstage and heat tolerance (HT) at anthesis. Survival rate of seedlings (SRS) and spikelet fertility (SF), the index traits of CTand HT, showed significant differences between the two parents under stresses. A total of four QTLs (qSRS1, qSRS7,qSRS11a and qSRS11b) for CT were identified on chromosomes 1, 7, 11, and the Xiushui 09 alleles increased SRS at all lociexcept qSRS7. Four QTLs for SF were identified on chromosomes 4, 5, 6, and 11. These QTLs could be classified into twomajor types based on their behaviors under normal and stress conditions. The first was QTL expressed only under normalcondition; and the second QTL was apparently stress induced and only expressed under stress. Among them, two QTLs(qSF4 and qSF6) which reduced the trait difference between heat stress and normal conditions must have contributed toHT because of their obvious contribution to trait stability, and the IR2061 allele at the qSF6 and the Xiushui 09 allele at the qSF4improved HT, respectively. No similar QTL was found between CT at seedling stage and HT at anthesis. Therefore, it ispossible to breed a new variety with CT and HT by pyramiding the favorable CT- and HT-improved alleles at above locifrom Xiushui 09 and IR2061, respectively, through marker-assisted selection (MAS).

关键词: cold tolerance, heat tolerance, advanced backcross population, QTL mapping, rice

Abstract: A set of 240 introgression lines derived from the advanced backcross population of a cross between a japonica cultivar,Xiushui 09, and an indica breeding line, IR2061, was developed to dissect QTLs affecting cold tolerance (CT) at seedlingstage and heat tolerance (HT) at anthesis. Survival rate of seedlings (SRS) and spikelet fertility (SF), the index traits of CTand HT, showed significant differences between the two parents under stresses. A total of four QTLs (qSRS1, qSRS7,qSRS11a and qSRS11b) for CT were identified on chromosomes 1, 7, 11, and the Xiushui 09 alleles increased SRS at all lociexcept qSRS7. Four QTLs for SF were identified on chromosomes 4, 5, 6, and 11. These QTLs could be classified into twomajor types based on their behaviors under normal and stress conditions. The first was QTL expressed only under normalcondition; and the second QTL was apparently stress induced and only expressed under stress. Among them, two QTLs(qSF4 and qSF6) which reduced the trait difference between heat stress and normal conditions must have contributed toHT because of their obvious contribution to trait stability, and the IR2061 allele at the qSF6 and the Xiushui 09 allele at the qSF4improved HT, respectively. No similar QTL was found between CT at seedling stage and HT at anthesis. Therefore, it ispossible to breed a new variety with CT and HT by pyramiding the favorable CT- and HT-improved alleles at above locifrom Xiushui 09 and IR2061, respectively, through marker-assisted selection (MAS).

Key words: cold tolerance, heat tolerance, advanced backcross population, QTL mapping, rice

CHENG Li-rui, Veronica Uzokwe, WANG Yun, ZHU Linghua. Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.)[J]. Journal of Integrative Agriculture, 2012, 12(3): 359-367.

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Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.)

Genetic Analysis of Cold Tolerance at Seedling Stage and Heat Tolerance atAnthesis in Rice (Oryza sativa L.)

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