Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

doi:10.1016/S2095-3119(13)60213-0

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (1): 127-135.DOI: 10.1016/S2095-3119(13)60213-0

Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

FANG Li-xiu, XIN Rui, CHEYi, XU Shi-qing

Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R.China

收稿日期:2011-07-20 出版日期:2013-01-01 发布日期:2013-01-24
通讯作者: Correspondence XU Shi-qing, Tel: +86-512-65880185, Fax: +86-512-65880186, E-mail: szsqxu@suda.edu.cn
基金资助:
This work was supported by the National Key Technology R&D Program of China (2007BAD72B01) and the National High Technology R&D Program of China (2011AA100306).

Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

FANG Li-xiu, XIN Rui, CHEYi, XU Shi-qing

Department of Applied Biology, Medical College, Soochow University, Suzhou 215123, P.R.China

Received:2011-07-20 Online:2013-01-01 Published:2013-01-24
Contact: Correspondence XU Shi-qing, Tel: +86-512-65880185, Fax: +86-512-65880186, E-mail: szsqxu@suda.edu.cn
Supported by:
This work was supported by the National Key Technology R&D Program of China (2007BAD72B01) and the National High Technology R&D Program of China (2011AA100306).

摘要/Abstract

摘要： Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-tofemale sex-reversal model of chick induced by diethylstilbestrol (DES) at onset of incubation (E0). The results of semiquantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d; the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene (Amh) expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos.

关键词: sex reversal , diethylstilbestrol , chicken , sex determination , sexual differentiation , sex-related gene

Abstract: Sex emerges out of a delicate dance between a variety of promale, anti-male, and possibly profemale genes. To investigate the role that sex-related genes play in sex determination and gonadal differentiation of fowl, we constructed a male-tofemale sex-reversal model of chick induced by diethylstilbestrol (DES) at onset of incubation (E0). The results of semiquantitative PCR showed that the expression of Sf1, the orphan nuclear receptor steroidogenic factor-1 gene, was put forward from E7d to E5d and up-regulated during E5-7d; the Dmrt1, the double sex and the Mab-3 related to transcription factor 1 gene, was down-regulated during E3-7d. Meanwhile, anti-Müllerian hormone gene (Amh) expressed at a similar level in the genetic females and sex-reversal females before E7d, while no expression products of the three female-specific genes Wpkci, Fet1 and Foxl2 were detected in male-to-female embryos. These findings suggest that the expression of some certain sex-related genes, induced by the exogenous estrogen during period of sex determination and gonadal differentiation, results in the male-to-female sex reversal. Moreover, high activity of Sf1 gene during E5-7d might be related to the profemale process, while low activity of Dmrt1 gene during E3-5d might be anti-male. The expression activity of Amh gene might only contribute to the promale process after E7d, however, it is possibly not an anti-female gene in chick embryos.

Key words: sex reversal , diethylstilbestrol , chicken , sex determination , sexual differentiation , sex-related gene

FANG Li-xiu, XIN Rui, CHEYi, XU Shi-qing. Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol[J]. Journal of Integrative Agriculture, 2013, 12(1): 127-135.

FANG Li-xiu, XIN Rui, CHEYi , XU Shi-qing. Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol[J]. Journal of Integrative Agriculture, 2013, 12(1): 127-135.

参考文献

[1]Berg C, Halldin K, Fridolfsson A K, Brandt I, Brunström B.1999. The avian egg as a test system for endocrinedisrupters : ef fects of di e thylst i lbest rol andethynylestradiol on sex organ development. Science ofthe Total Environment, 233, 57-66

[2]Ellegren H. 2002. Dosage compensation: do birds do it aswell? Trends in Genetics, 18, 25-28

[3]Feng Y, Zhang S, Peng X, Yuan J, Yang Y, Zhan H, Gong Y.2007. Expression analysis of genes putatively involvedin chicken gonadal development. Acta BiologicaHungarica, 58, 163-172

[4]Ferguson-Smith M. 2007. The evolution of sexchromosomes and sex determination in vertebrates andthe key role of DMRT. Sexual Development, 1, 2-11

[5]Govoroun M S, Pannetier M, Pailhoux E, Cocquet J, BrillardJ P, Couty I, Batellier F, Cotinot C. 2004. Isolation ofchicken homolog of the FOXL2 gene and comparisonof its expression patterns with those of aromatase duringovarian development. Developmental Dynamics, 231,859-870

[6]Guichard A, Cedard L, Mignot T M, Scheib D, Haffen K A.1979. Radioimmunoassay of steroids produced by chickembryo gonads cultured in the presence of someexogenous steroid precursors. General andComparative Endocrinology, 39, 9-19

[7]Hudson Q J, Smith C A, Sinclair A H. 2005. Aromataseinhibition reduces expression of FOXL2 in theembryonic chicken ovary. Developmental Dynamics,233, 1052-1055

[8]Ikeda Y, Shen W H, Ingraham H A, Parker K L.1994.Developmental expression of mouse steroidogenicfactor-1, an essential regulator of the steroidhydroxylases

[9]Molecular Endocrinology, 8, 654-662

[10]Kent J, Wheatley S C, Andrews J E, Sinclair A H, KoopmanP. 1996. A male-specific role for SOX9 in vertebrate sexdetermination. Development, 122, 2813-2822

[11]Kozelka A W, Gallagher T F. 1934. Effect of male hormoneextracts, theelin, and theelol on the chick embryo.Proceedings of the Society for Experimental Biologyand Medicine, 31, 1143-1144

[12]Luo X, Ikeda Y, Parker K L. 1994. A cell-specific nuclear receptor is essential for adrenal and gonadaldevelopment and sexual differention. Cell, 77, 481-490

[13]McQueen H A, Mc Bride D, Miele G, Bird A P, Clinton M.2001. Dosage compensation in birds. Current Biology,11,253-257

[14]Münsterberg A, Lovell-Badge R. 1991. Expression of themouse anti-Müllerian hormone gene suggests a role inboth male and female sex differentiation. Development,113, 613-624

[15]Nachtigal M W, Hirokawa Y, Enyeart-Van Houten D L,Flanagan J N, Hammer G D, Ingraham HA. 1998.Wilms’tumor 1 and Dax-1 modulate the orphan nuclear receptorSF-1 in sex-specific gene expression

[16]Cell, 93, 445-454

[17]Nishikimi H, Kansaku N, Saito N, Usami M, Ohno Y,Shimada K. 2000. Sex differentiation and mRNAexpression of P450c17, P450arom and AMH in gonadsof the chicken. Molecular Reproduction andDevelopment, 55, 20-30

[18]Orlando E F, Guillette Jr L J. 2007. Sexual dimorphicresponses in wildlife exposed to endocrine disruptingchemicals. Environmental Research, 104, 163-173

[19]Raymond C S, Kettlewell J R, Hirsch B, Bardwell V J,Zarkower D. 1999. Expression of Dmrt1 in the genitalridge of mouse and chicken embryos suggests a role invertebrate sexual development. Developmental Biology,215, 208-220

[20]Reed K J, SinclairAH. 2002. FET-1: a novelW-linked, femalespecific gene up-regulated in the embryonic chickenovary Mechanical Engineering, 119, S87-S90.

[21]de Santa Barbara P, Bonneaud N, Boizet B, Desclozeaux M,Moniot B, Sudbeck P, Scherer G, Poulat F, Berta P. 1998.Direct interaction of Sry-related protein SOX9 andsteroidogenic factor 1 regulates transcription of thehuman anti-müllerian hormone gene. Molecular andCellular Biology, 18, 6653-6665

[22]Scheib D 1983. Effects and role of estrogens in aviangonadal differentiation. Differentiation, 23, 87-92

[23]Shan Z, Nanda I, Wang Y, Schmid M, Vortkamp A, Haaf T.2000. Sex-specific expression of an evolutionarilyconserved male regulatory gene, DMRT1, in birds.Cytogenetics and Cell Genetics, 89, 252-257

[24]Smith CA, KatzM, SinclairAH. 2003. DMRT1 is upregulatedin the gonads during female-to-male sex reversal in ZWchicken embryos. Biology of Reproduction, 68, 560-570

[25]Smith CA,Matthijs J S,Andrew H S. 1999a. Gene expressionduring gonadogenesis in the chicken embryo. Gene,234, 395-402

[26]Smith C A, Smith M J, Sinclair A H. 1999b. Expression ofchicken steroidogenic factor-1 during gonadal sexdif ferentiation General and Comparati v eEndocrinology, 113, 187-196

[27]Vaillant S, Magre S, Dorizzi M, Pieau C, Richard-Mercier N.2001. Expression of AMH, SF1, and SOX9 in gonads ofgenetic female chickens during sex reversal induced byan aromatase inhibitor. Developmental Dynamics, 222,228-237

[28]WallisM C,Waters P D, Graves JA. 2008. Sex determinationin mammals - before and after the evolution of SRY.Cellular and Molecular Life Sciences, 65, 3182.

[29]Zheng J X, Yang N. 2007. mRNA expression of genes relatedto avian sex determination during female-to-male sexreversal in ZW chicken embryos. Yi Chuan, 29, 81-86

[30](in Chinese)Zhou M, Maeda T, Xu S Q. 2008. Effect of diethylstilbestrolon feminization of chicken fertile eggs. Bulletin ofScience and Technology, 24, 177-182. (in Chinese)

Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

Expression of Sex-RelatedGenes in Chicken Embryos During Male-to-Female Sex Reversal Exposure to Diethylstilbestrol

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 12

编辑推荐

Metrics

[1]	SUN Cong-jiao, DUaN Zhong-yi, QU Lu-jiang, ZhENg Jiang-xia, YaNg Ning, XU gui-yun. Expression analysis for candidate genes associated with eggshell mechanical property[J]. Journal of Integrative Agriculture, 2016, 15(2): 397-402.
[2]	ZHANG Wen-wu, KONG Li-na, ZHANG De-xiang, JI Cong-liang, ZHANG Xi-quan, LUO Qing-bin. Effect of the C.–1 388 A>G polymorphism in chicken heat shock transcription factor 3 gene on heat tolerance[J]. Journal of Integrative Agriculture, 2015, 14(9): 1808-1815.
[3]	ZHANG Lei, ZUO Qi-sheng, LI Dong, LIAN Chao, Kamel E Ahmed, TANG Bei-bei, SONG Jiu-zhou, ZHANG Ya-ni, LI Bi-chun. Study on the role of JAK/STAT signaling pathway during chicken spermatogonial stem cells generation based on RNA-Seq[J]. Journal of Integrative Agriculture, 2015, 14(5): 939-948.
[4]	Zhao Guang-wei, WanG Shuai, WanG Wang, ZhanG Zhen-chao, XIe Qing, ZhanG Meng, I a hassan, Yan Ruo-feng, SonG Xiao-kai, Xu Li-xin, LI Xiang-rui. Type I strain of Toxoplasma gondii from chicken induced different immune responses with that from human, cat and swine in chicken[J]. Journal of Integrative Agriculture, 2015, 14(5): 956-965.
[5]	LI Ling-ling , LI Shu-tian. Nitrogen Mineralization from Animal Manures and Its Relation to Organic N Fractions[J]. Journal of Integrative Agriculture, 2014, 13(9): 2040-2048.
[6]	ZHOU Zuo-yong, HU Shi-jun, WANG Zhi-ying, GUO Zhi-li, QIN Bo , NIE Kui. Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella[J]. Journal of Integrative Agriculture, 2014, 13(4): 904-910.
[7]	YANG Xiu-rong, JIANG He-sheng, ZHENG Jiang-xia, QU Lu-jiang, CHEN Si-rui, LI Jun-ying, XU Guiyun, YANG Ning. Dosage Effects of Fadrozole on Growth and Development of Sex-Reversed Genetic Female Chickens[J]. Journal of Integrative Agriculture, 2013, 12(6): 1049-1053.
[8]	OUYANG Jian-hua, XIE Liang, NIE Qing-hua, ZENG Hua, PENG Zhi-jun, ZHANG De-xiang, ZHANG Xi-quan. The Effects of Different Sex-Linked Dwarf Variations on Chinese Native Chickens[J]. Journal of Integrative Agriculture, 2012, 12(9): 1500-1508.
[9]	ZHAO Guang-wei, SHEN Bo, XIE Qing, XU Li-xin, YAN Ruo-feng, SONG Xiao-kai, Hassan Ibrahim Adam, LI Xiang-rui. Isolation and Molecular Characterization of Toxoplasma gondii from Chickens in China[J]. Journal of Integrative Agriculture, 2012, 12(8): 1347-1353.
[10]	SUN Gui-rong, LI Yan, KANG Xiang-tao, TIAN Ya-dong, ZHANG Hu , LI Kui. Effect of Beak Trimming Stress on the Apoptosis and Its Related Protein Expression of Chicken Spleen[J]. Journal of Integrative Agriculture, 2012, 12(4): 639-645.
[11]	ZHU Jia-ting, FENG Min, YAN Jian-min, LIU Chun-quan, HA Yi-ming, GAO Mei-xu, YANG Ping, WANG Zhi-dong, WANG De-ning, LI Shu-rong, GU Gui-qiang. Toxicological Evaluation of Chicken-Breast Meat with High-Dose Irradiation[J]. Journal of Integrative Agriculture, 2012, 12(12): 2088-2096.
[12]	CHEN Juan, MA Hai-tian, WANG Man, KONG Yi-li , ZOU Si-xiang. Creatine Pyruvate Enhances Lipolysis and Protein Synthesis in Broiler Chicken[J]. Journal of Integrative Agriculture, 2011, 10(12): 1977-1985.