Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

doi:10.1016/S2095-3119(14)60746-2

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (1): 114-124.DOI: 10.1016/S2095-3119(14)60746-2

Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

WU Xiao-hu, XU Jun, LIU Yong-zhuo, DONG Feng-shou, LIU Xin-gang, ZHANG Wen-wen, ZHENG Yong-quan

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

收稿日期:2013-11-20 出版日期:2015-01-01 发布日期:2015-01-08
通讯作者: ZHENG Yong-quan, Tel/Fax: +86-10-62815908, E-mail: zhengyongquan@ippcaas.cn
作者简介:WU Xiao-hu, Mobile: 18801027695, E-mail: taianwuhu@163.com;* These authors contributed equally to this paper.
基金资助:
This study was supported by the National Natural Science Foundation of China (31171879 and 31000863) and the Special Fund for Agro-Scientific Research in the Public Interest, China (201203098).

Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

WU Xiao-hu, XU Jun, LIU Yong-zhuo, DONG Feng-shou, LIU Xin-gang, ZHANG Wen-wen, ZHENG Yong-quan

State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

Received:2013-11-20 Online:2015-01-01 Published:2015-01-08
Contact: ZHENG Yong-quan, Tel/Fax: +86-10-62815908, E-mail: zhengyongquan@ippcaas.cn
About author:WU Xiao-hu, Mobile: 18801027695, E-mail: taianwuhu@163.com;* These authors contributed equally to this paper.
Supported by:
This study was supported by the National Natural Science Foundation of China (31171879 and 31000863) and the Special Fund for Agro-Scientific Research in the Public Interest, China (201203098).

摘要/Abstract

摘要： The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid (PLFA) and community-level physiological profiles (CLPPs). Our results demonstrated that substrate-induced respiration (on day 15) and microbial biomass C (on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients (qCO2) and respiratory quotients (QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass (both Gram-positive bacteria (GP) and Gram-negative bacteria (GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. Fluxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input (75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis (PCA) of the PLFAs revealed that fluxapyroxad treatment significantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development (AWCD) and functional diversity index (H´) were increased only on day 60. In addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested.

关键词: fluxapyroxad , microbial activity , community structure , functional diversity

Abstract: The aim of this work was to assess the effect of applying three different doses of fluxapyroxad on microbial activity, community structure and functional diversity as measured by respiration, microbial biomass C, phospholipid fatty acid (PLFA) and community-level physiological profiles (CLPPs). Our results demonstrated that substrate-induced respiration (on day 15) and microbial biomass C (on days 7 and 15) were inhibited by fluxapyroxad, but stimulation was observed thereafter. In contrast, fluxapyroxad addition increased the basal respiration and metabolic quotients (qCO2) and respiratory quotients (QR). Analysis of the PLFA profiles revealed that the total and bacterial biomass (both Gram-positive bacteria (GP) and Gram-negative bacteria (GN)) were decreased within the initial 15 days, whereas those as well as the GN/GP ratio were increased at days 30 and 60. Fluxapyroxad input decreased the fungi biomass but increased the bacteria/fungi ratio at all incubation time. Moreover, high fluxapyroxad input (75 mg fluxapyroxad kg–1 soil dry weight) increased the microbial stress level. A principal component analysis (PCA) of the PLFAs revealed that fluxapyroxad treatment significantly shifted the microbial community structure, but all of the observed effects were transient. Biolog results showed that average well color development (AWCD) and functional diversity index (H´) were increased only on day 60. In addition, the dissipation of fluxapyroxad was slow in soil, and the degradation half-lives varied from 158 to 385 days depending on the concentration tested.

Key words: fluxapyroxad , microbial activity , community structure , functional diversity

WU Xiao-hu, XU Jun, LIU Yong-zhuo, DONG Feng-shou, LIU Xin-gang, ZHANG Wen-wen, ZHENG Yong-quan. Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil[J]. Journal of Integrative Agriculture, 2015, 14(1): 114-124.

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Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

Impact of fluxapyroxad on the microbial community structure and functional diversity in the silty-loam soil

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