Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (2): 415-425.DOI: 10.1016/S2095-3119(13)60424-4

• 论文 • 上一篇    下一篇

Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil

 LIU Ling-zhi, QIN Si-jun, LÜ De-guo, WANG Bing-ying , YANG Ze-yuan   

  1. 1.Key Laboratory for Northern Fruit Cultivation and Physio-Ecology of Shenyang City/College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China
    2.College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, P.R.China
    3.Foreign Language Department, Shenyang Agricultural University, Shenyang 110866, P.R.China
  • 收稿日期:2012-10-31 出版日期:2014-02-01 发布日期:2014-02-06
  • 通讯作者: QIN Si-jun, E-mail: qinsijun1975@163.com; Lü De-guo, Tel/Fax: +86-24-88487219, E-mail: shynydxgshzp@163.com
  • 作者简介:LIU Ling-zhi, Mobile: 13238832696, E-mail: liulingzhi74@163.com

Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil

 LIU Ling-zhi, QIN Si-jun, LÜ De-guo, WANG Bing-ying , YANG Ze-yuan   

  1. 1.Key Laboratory for Northern Fruit Cultivation and Physio-Ecology of Shenyang City/College of Horticulture, Shenyang Agricultural University, Shenyang 110866, P.R.China
    2.College of Land and Environment, Shenyang Agricultural University, Shenyang 110866, P.R.China
    3.Foreign Language Department, Shenyang Agricultural University, Shenyang 110866, P.R.China
  • Received:2012-10-31 Online:2014-02-01 Published:2014-02-06
  • Contact: QIN Si-jun, E-mail: qinsijun1975@163.com; Lü De-guo, Tel/Fax: +86-24-88487219, E-mail: shynydxgshzp@163.com
  • About author:LIU Ling-zhi, Mobile: 13238832696, E-mail: liulingzhi74@163.com

PDF

摘要： In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4 +-N content, NO3 --N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil. The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the β-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus. Therefore, soil-based environmental factors, such as pH variation and content of NH4 +-N and NO3 --N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.

关键词: apple orchard soil , ammonia-oxidizing bacteria , potential nitrification , community structure , PCR-denaturing gradient gel electrophoresis

Abstract: In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria (AOB) in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis (DGGE). We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen (N) content, NH4 +-N content, NO3 --N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil. The Shannon-Winner diversity, as well as species richness and evenness indices (determined by PCR-DGGE banding patterns) in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the β-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus. Therefore, soil-based environmental factors, such as pH variation and content of NH4 +-N and NO3 --N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.

Key words: apple orchard soil , ammonia-oxidizing bacteria , potential nitrification , community structure , PCR-denaturing gradient gel electrophoresis