Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

doi:10.1016/S2095-3119(13)60396-2

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (11): 2103-2113.DOI: 10.1016/S2095-3119(13)60396-2

Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

ZHANG Chao, LIU Guo-bin, XUE Sha , XIAO Lie

1.State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, P.R.China
2.Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, P.R.China

收稿日期:2012-10-23 出版日期:2013-11-01 发布日期:2013-12-08
通讯作者: Correspondence XUE Sha, Mobile: 13679211517, E-mail: xuesha100@163.com
作者简介:ZHANG Chao, Mobile: 13669200244, E-mail: zhangchaolynn@163.com
基金资助:
This work was supported by the Strategic Technology Project of Chinese Academy of Sciences (XDA05060300), and the Science and Technology Research and Development Program of Shaanxi Province, China (2011KJXX63).

Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

ZHANG Chao, LIU Guo-bin, XUE Sha , XIAO Lie

1.State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling 712100, P.R.China
2.Institute of Soil and Water Conservation, Chinese Academy of Sciences and Ministry of Water Resources, Yangling 712100, P.R.China

Received:2012-10-23 Online:2013-11-01 Published:2013-12-08
Contact: Correspondence XUE Sha, Mobile: 13679211517, E-mail: xuesha100@163.com
About author:ZHANG Chao, Mobile: 13669200244, E-mail: zhangchaolynn@163.com
Supported by:
This work was supported by the Strategic Technology Project of Chinese Academy of Sciences (XDA05060300), and the Science and Technology Research and Development Program of Shaanxi Province, China (2011KJXX63).

摘要/Abstract

摘要： The Loess Plateau in China is one of the most eroded areas in the world. Accordingly, vegetation restoration has been implemented in this area over the past two decades to remedy the soil degradation problem. Understanding the microbial community structure is essential for the sustainability of ecosystems and for the reclamation of degraded arable land. This study aimed to determine the effect of different vegetation types on microbial processes and community structure in rhizosphere soils in the Loess Plateau. The six vegetation types were as follows: two natural grassland (Artemisia capillaries and Heteropappus altaicus), two artificial grassland (Astragalus adsurgens and Panicum virgatum), and two artificial shrubland (Caragana korshinskii and Hippophae rhamnoides) species. The microbial community structure and functional diversity were examined by analyzing the phospholipid fatty acids (PLFAs) and community-level physiological profiles. The results showed that rhizosphere soil sampled from the H. altaicus and A. capillaries plots had the highest values of microbial biomass C, average well color development of carbon resources, Gram-negative (G-) bacterial PLFA, bacterial PLFA, total PLFA, Shannon richness, and Shannon evenness, as well as the lowest metabolic quotient. Soil sampled from the H. rhamnoides plots had the highest metabolic quotient and Gram-positive (G+) bacterial PLFA, and soil sampled from the A. adsurgens and A. capillaries plots had the highest fungal PLFA and fungal:bacterial PLFA ratio. Correlation analysis indicated a significant positive relationship among the microbial biomass C, G- bacterial PLFA, bacterial PLFA, and total PLFA. In conclusion, plant species under arid climatic conditions significantly affected the microbial community structure in rhizosphere soil. Among the studied plants, natural grassland species generated the most favorable microbial conditions.

关键词: soil microbial biomass , microbial community structure , PLFA , community-level physiological profiles , vegetation types

Abstract: The Loess Plateau in China is one of the most eroded areas in the world. Accordingly, vegetation restoration has been implemented in this area over the past two decades to remedy the soil degradation problem. Understanding the microbial community structure is essential for the sustainability of ecosystems and for the reclamation of degraded arable land. This study aimed to determine the effect of different vegetation types on microbial processes and community structure in rhizosphere soils in the Loess Plateau. The six vegetation types were as follows: two natural grassland (Artemisia capillaries and Heteropappus altaicus), two artificial grassland (Astragalus adsurgens and Panicum virgatum), and two artificial shrubland (Caragana korshinskii and Hippophae rhamnoides) species. The microbial community structure and functional diversity were examined by analyzing the phospholipid fatty acids (PLFAs) and community-level physiological profiles. The results showed that rhizosphere soil sampled from the H. altaicus and A. capillaries plots had the highest values of microbial biomass C, average well color development of carbon resources, Gram-negative (G-) bacterial PLFA, bacterial PLFA, total PLFA, Shannon richness, and Shannon evenness, as well as the lowest metabolic quotient. Soil sampled from the H. rhamnoides plots had the highest metabolic quotient and Gram-positive (G+) bacterial PLFA, and soil sampled from the A. adsurgens and A. capillaries plots had the highest fungal PLFA and fungal:bacterial PLFA ratio. Correlation analysis indicated a significant positive relationship among the microbial biomass C, G- bacterial PLFA, bacterial PLFA, and total PLFA. In conclusion, plant species under arid climatic conditions significantly affected the microbial community structure in rhizosphere soil. Among the studied plants, natural grassland species generated the most favorable microbial conditions.

Key words: soil microbial biomass , microbial community structure , PLFA , community-level physiological profiles , vegetation types

ZHANG Chao, LIU Guo-bin, XUE Sha , XIAO Lie. Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China[J]. Journal of Integrative Agriculture, 2013, 12(11): 2103-2113.

参考文献

[1]An S S, Huang Y M, Zheng F L. 2009. Evaluation of soilmicrobial indices along a revegetation chronosequencein grassland soils on the Loess Plateau, Northwest China.Applied Soil Ecology, 41, 286-292

[2]Anderson J P E, Domsch K H. 1975. Measurement ofbacterial and fungal contributions to respiration ofselected agricultural and forest soils. Canadian Journalof Microbiology, 21, 314-322

[3]Anderson T H, Domsch K H. 1993. The metabolic quotientfor CO2 (qCO2) as a specific activity parameter to assessthe effects of environmental conditions, such as pH,on the microbial biomass of the soil. Soil Biology &Biochemistry, 25, 393-395

[4]Bardgett R D, Hobbs P J, Frostegård A. 1996. Changes insoil fungal: bacterial biomass ratios following reductionsin the intensity of management of an upland grassland.Biology and Fertility of Soils, 22, 261-264

[5]Bird J A, Herman D J, Firestone M K. 2011. Rhizospherepriming of soil organic matter by bacterial groups in agrassland soil. Soil Biology & Biochemistry, 43, 718-725

[6]Bligh E G, Dyer W J. 1959. A rapid method of total lipidextraction and purification. Canadian Journal ofBiochemistry and Physiology, 37, 911-917

[7]Bossio D A, Scow K M. 1995. Impact of carbon and?ooding on the metabolic diversity of microbialcommunities in soils. Applied and EnvironmentalMicrobiology, 61, 4043-4050

[8]Cao C Y, Jiang D M, Teng X H, Jiang Y, Liang W J, Cui ZB. 2008. Soil chemical and microbiological propertiesalong a chronosequence of Caragana microphylla Lam.plantations in the Horqin sandy land of Northeast China.Applied Soil Ecology, 40, 78-85

[9]Chapman S J, Campbell C D, Artz R R E. 2007. AssessingCLPPs using MicroRespTM. A comparison with Biologand multi-SIR. Journal of Soils Sediments, 7, 406-410

[10]Chen L D, Gong J, Fu B J, Huang Z L, Huang Y L, Gui LD. 2007. Effect of land use conversion on soil organiccarbon sequestration in the loess hilly area, LoessPlateau of China. Ecological Research, 22, 641-648

[11]Chen M M, Zhu Y G, Su Y H, Chen B D, Fu B J, MarschneP. 2007. Effects of soil moisture and plant interactionson the soil microbial community structure. EuropeanJournal of Soil Biology, 43, 31-38

[12]Esperschütz J, Buegger F, Winkler J B, Munch J C, SchloterM, Gattinger A 2009. Microbial response to exudates inthe rhizosphere of young beech trees (Fagus sylvatica L.)after dormancy. Soil Biology & Biochemistry, 41, 1976-1985Federle T W

[13]1986. Microbial distribution in soil - newtechniques. In: Megusar F, Gantar M, eds., Perspectivesin Microbial Ecology. Slovene Society for Microbiology,Ljubljana. pp. 493-498

[14]Florgard C. 2004. Remaining original natural vegetation intowns and cities. Urban Forestry & Urban Greening, 3,1-2

[15]Frostegård A, Bååth E, Tunlid A. 1993. Shifts in thestructure of soil microbial communities in limed forestsas revealed by phospholipid fatty acid analysis. SoilBiology & Biochemistry, 25, 723-730

[16]Fu B J, Wang Y F, Lu Y H, He C S, Chen L D, Song CJ. 2009. The effects of land-use combinations on soilerosion: a case study in the Loess Plateau of China.Progress in Physical Geography, 33, 793-804

[17]Garcia C, Roldan A, Hernandez T. 2005. Ability of differentplant species to promote microbiological processes insemiarid soil. Geoderma, 124, 193-202

[18]Grayston S J, Grif?th G S, Mawdsley J L, Campbell C D,Bardgett R D. 2001. Accounting for variability in soilmicrobial communities of temperate upland grasslandecosystems. Soil Biology & Biochemistry, 33, 533-551

[19]Grayston S J, Vaughan D, Jones D. 1997. Rhizospherecarbon flow in trees, in comparison with annual plants:the importance of root exudation and its impact onmicrobial activity and nutrient availability. Applied SoilEcology, 5, 29-56

[20]Hamer U, Makeschin F. 2009. Rhizosphere soil microbialcommunity structure and microbial activity in set-aside and intensively managed arable land. Plant and Soil,316, 57-69

[21]Hinsinger P, Bengough A G, Vetterlein D, Young I M.2009. Rhizosphere: biophysics, biogeochemistry andecological relevance. Plant and Soil, 321, 117-152

[22]Innes L, Hobbs P J, Bardgett R D. 2004. The impactsof individual plant species on rhizosphere microbialcommunities in soils of different fertility. Biology andFertility of Soils, 40, 7-13

[23]Jenkinson D S, Powlson D S. 1976. The effects of biocidaltreatments on metabolism in soils - a method formeasuring soil biomass. Soil Biology & Biochemistry, 8,167-177

[24]Klironomos J N. 2002. Feedback with soil biota contributesto plant rarity and invasiveness in communities. Nature,417, 67-70

[25]Mariotte C A, Hudson G, Hamilton D, Neilson R, Boag B,Handley L L, Wishart J, Scrimgeour C M, Robinson D.1997. Spatial variability of soil total C and N and theirstable isotopes in an upland Scottish grassland. Plantand Soil, 196, 151-162

[26]Meharg A A, Killham K. 1990. The effect of soil pH onrhizosphere carbon ?ow of Lolium perenne. Plant andSoil, 123, 1-7

[27]Montealegre C M, van Kessel C, Russelle M P, Sadowsky MJ. 2002. Changes in microbial activity and compositionin a pasture ecosystem exposed to elevated atmosphericcarbon dioxide. Plant and Soil, 243, 197-207

[28]Nguyen C. 2003. Rhizodeposition of organic C by plants:mechanisms and control. Agronomie, 23, 375-396

[29]Paterson E, Gebbing T, Abel C, Sim A, Telfer G. 2007.Rhizodeposition shapes rhizosphere microbialcommunity structure in organic soil. New Phytologist,173, 600-610

[30]Petersen H, Luxton M. 1982. A comparative analysis ofsoil faunal populations and their role in decompositionprocesses. Oikos, 39, 287-388

[31]Richard T C, Peter Dalla-Bettab, Carole C K, Jeffrey M K.2004. Controls on soil respiration in semiarid soils. SoilBiology & Biochemistry, 36, 945-951

[32]Ridder-Duine A S, Kowalchuk G A, Klein Gunnewiek P J A,Smant W, van Veen J A, de Boer W. 2005. Rhizsopherebacterial community composition in natural stands ofCarex arenaria (sand sedge) is determined by bulk soilcommunity composition. Soil Biology & Biochemistry,37, 349-357

[33]Shannon C. 1948. A mathematical theory of communication.The Bell System Technical Journal, 27, 379-423

[34]Sinha S, Masto R E, Ram L C, Selvi V A, Srivastava N K,Tripathi R C, George J. 2009. Rhizosphere soil microbialindex of tree species in a coal mining ecosystem. SoilBiology & Biochemistry, 41, 1824-1832

[35]Tscherko D, Ute H, Marie-Claude M, Ellen K. 2004. Shiftsin rhizosphere microbial communities and enzymeactivity of Poa alpina across an alpine chronosequence.Soil Biology & Biochemistry, 36, 1685-1698

[36]Vance E D, Brookes P C, Jenkinson D. 1987. An extractionmethod for measuring microbial biomass carbon. SoilBiology & Biochemistry, 19, 703-707

[37]Whisenant S G. 1995. Landscape dynamics and arid landrestoration. In: Roundy B R, McArthur E D, Haley J S,Mann D K, eds., Proceedings: Wildlife Shrub and AridLand Restoration Symposium. USDA, Ogden, USA. pp.26-34

[38]Winding A, Hund-Rinke K, Rutgers M. 2005. The use ofmicroorganisms in ecological soil classi?cation andassessment concepts. Ecotoxicology and EnvironmentalSafety, 62, 230-248

[39]Xiao L, Liu G B, Xue S, Zhang C. 2013. Soil microbialcommunity composition during natural recovery in theLoess Plateau, China. Journal of Integrative Agriculture,12, 1872-1883

[40]Zelles L. 1998. Fatty acid patterns of phospholipids andlipopolysaccharides in the characterisation of microbialcommunities in soil: a review. Biology and Fertility ofSoils, 29, 111-129

[41]Zhang C, Liu G B, Xue S, Song Z L. 2011a. A comparisonof soil qualities of different revegetation types in theLoess Plateau, China. Plant and Soil, 347, 163-178

[42]Zhang C, Liu G B, Xue S, Song Z L. 2011b. Rhizospheresoil microbial activity under different vegetation typeson the Loess Plateau, China. Geoderma, 161, 115-125

[43]Zhang C, Liu G B, Xue S. 2012. Rhizosphere soil microbialproperties on abandoned croplands in the Loess Plateau,China during vegetation succession. European Journalof Soil Biology, 50, 127-136

[44]Zhu B B, Li Z B, Li P, Liu G B, Xue S. 2010. Soilerodibility, microbial biomass, and physical-chemicalproperty changes during long-term natural vegetationrestoration: a case study in the Loess Plateau, China.Ecological Research, 25, 531-541

Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

Effect of Different Vegetation Types on the Rhizosphere Soil Microbial Community Structure in the Loess Plateau of China

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