Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella

doi:10.1016/S2095-3119(13)60384-6

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (4): 904-910.DOI: 10.1016/S2095-3119(13)60384-6

Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella

ZHOU Zuo-yong, HU Shi-jun, WANG Zhi-ying, GUO Zhi-li, QIN Bo , NIE Kui

1、Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, P.R.China
2、College of Animal Science and Technology, Southwest University, Chongqing 400715, P.R.China

收稿日期:2012-10-11 出版日期:2014-04-01 发布日期:2014-04-16
通讯作者: NIE Kui, Tel/Fax: +86-23-68251196, E-mail: niekui@126.com
作者简介:ZHOU Zuo-yong, Tel: +86-23-46751100, E-mail: zzyxnny@163.com
基金资助:
This work was supported by the Fundamental Research Funds for the Central Universities, China (XDJK2010C099), the Science Fundation for Young Scientists of Southwest University, China (QNRC200804) and the Scientific Research Fund of Veterinary Medicine Department of Southwest University, China.

Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella

ZHOU Zuo-yong, HU Shi-jun, WANG Zhi-ying, GUO Zhi-li, QIN Bo , NIE Kui

1、Department of Veterinary Medicine, Rongchang Campus of Southwest University, Chongqing 402460, P.R.China
2、College of Animal Science and Technology, Southwest University, Chongqing 400715, P.R.China

Received:2012-10-11 Online:2014-04-01 Published:2014-04-16
Contact: NIE Kui, Tel/Fax: +86-23-68251196, E-mail: niekui@126.com
About author:ZHOU Zuo-yong, Tel: +86-23-46751100, E-mail: zzyxnny@163.com
Supported by:
This work was supported by the Fundamental Research Funds for the Central Universities, China (XDJK2010C099), the Science Fundation for Young Scientists of Southwest University, China (QNRC200804) and the Scientific Research Fund of Veterinary Medicine Department of Southwest University, China.

摘要/Abstract

摘要： Toll-like receptors (TLRs) are a group of highly conserved molecules which initiate the innate immune response to pathogens by recognizing structural motifs of microbes. Understanding the changes in chicken Toll-like receptors (ChTLRs) and signal adaptors expression that occur with Eimeria tenella infection will help to elucidate the molecular basis of immune control of coccidiosis caused by Eimeria. The present study detected the dynamic changes in the expression of ChTLRs and associated signal adaptors in the spleen and cecum of E. tenella-infected chickens during the early stage of infection. The results showed that the expression peak for ChTLRs, MyD88 and TRIF occurred at 12 h post-infection (hpi), ChTLR3, ChTLR15 and MyD88 mRNA expression in the spleen of E. tenella infected chickens were significantly higher (P<0.05) than that of negative control chickens, and there were similar tendencies of these molecules expression in the cecum and spleen of E. tenella-infected chickens. The expression of MyD88 was upregulated at four time points in the cecum of E. tenella-infected chickens. The results of this study indicate that ChTLR3, ChTLR15 and MyD88 play a role in young chickens infected with E. tenella.

关键词: Eimeria tenella , signal adaptors , Toll-like receptors , spleen and cecum , chicken

Abstract: Toll-like receptors (TLRs) are a group of highly conserved molecules which initiate the innate immune response to pathogens by recognizing structural motifs of microbes. Understanding the changes in chicken Toll-like receptors (ChTLRs) and signal adaptors expression that occur with Eimeria tenella infection will help to elucidate the molecular basis of immune control of coccidiosis caused by Eimeria. The present study detected the dynamic changes in the expression of ChTLRs and associated signal adaptors in the spleen and cecum of E. tenella-infected chickens during the early stage of infection. The results showed that the expression peak for ChTLRs, MyD88 and TRIF occurred at 12 h post-infection (hpi), ChTLR3, ChTLR15 and MyD88 mRNA expression in the spleen of E. tenella infected chickens were significantly higher (P<0.05) than that of negative control chickens, and there were similar tendencies of these molecules expression in the cecum and spleen of E. tenella-infected chickens. The expression of MyD88 was upregulated at four time points in the cecum of E. tenella-infected chickens. The results of this study indicate that ChTLR3, ChTLR15 and MyD88 play a role in young chickens infected with E. tenella.

Key words: Eimeria tenella , signal adaptors , Toll-like receptors , spleen and cecum , chicken

ZHOU Zuo-yong, HU Shi-jun, WANG Zhi-ying, GUO Zhi-li, QIN Bo , NIE Kui. Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella[J]. Journal of Integrative Agriculture, 2014, 13(4): 904-910.

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Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella

Expression of Chicken Toll-Like Receptors and Signal Adaptors in Spleen and Cecum of Young Chickens Infected with Eimeria tenella

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