Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (10): 2170-2176.DOI: 10.1016/S2095-3119(14)60838-8

• 论文 • 上一篇    下一篇

Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus

 WEI Fang, HU Jie, CUI Ming-zhu, ZHANG Yan-hui, LI Yun-ling , TIAN Bao-ming   

  1. 1、School of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.China
    2、Zhengzhou Key Laboratory of Plant Molecular Breeding, Zhengzhou 450001, P.R.China
  • 收稿日期:2013-09-24 出版日期:2014-10-01 发布日期:2014-10-10
  • 通讯作者: TIAN Bao-ming, Tel/Fax: +86-371-67739513, E-mai: tianbm@zzu.edu.cn
  • 作者简介:WEI Fang, E-mail: fangwei@zzu.edu.cn; These authors contributed equally to this study.
  • 基金资助:

    The authors are grateful to a grant of the National Key Technology R&D Program of China (2010BAD01B02) and a fund from the foundation of Postgraduate Research Program of Zhengzhou University, China (12L00403).

Global Analysis of Cytosine Methylation and Proteome Under Cold Treatment in Brassica napus

 WEI Fang, HU Jie, CUI Ming-zhu, ZHANG Yan-hui, LI Yun-ling , TIAN Bao-ming   

  1. 1、School of Life Sciences, Zhengzhou University, Zhengzhou 450001, P.R.China
    2、Zhengzhou Key Laboratory of Plant Molecular Breeding, Zhengzhou 450001, P.R.China
  • Received:2013-09-24 Online:2014-10-01 Published:2014-10-10
  • Contact: TIAN Bao-ming, Tel/Fax: +86-371-67739513, E-mai: tianbm@zzu.edu.cn
  • About author:WEI Fang, E-mail: fangwei@zzu.edu.cn; These authors contributed equally to this study.
  • Supported by:

    The authors are grateful to a grant of the National Key Technology R&D Program of China (2010BAD01B02) and a fund from the foundation of Postgraduate Research Program of Zhengzhou University, China (12L00403).

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摘要： Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosine methylation and proteome were analysed in B. napus after cold treatment by methylation-sensitive amplified polymorphism (MSAP) and two-dimensional protein electrophoresis technology (2-DE). The results showed that the lowered genome-wide DNA methylation status was revealed after cold treatment, and about 0.88% of discrepancy in DNA methylation was detected between the non-flowering and flowering plants after cold treatment. Moreover, the 52 significantly up-regulated proteins emerged in comparison with the 36 down-regulated proteins, as well as the 14 proteins exclusively detected in the flowering plants. Intriguingly the 8 specifically expressed proteins in the non-flowering plants disappeared in the flowering plants with cold treatment. Therefore, these present data proved that the correlating changes of cytosine methylation and proteomic expression were evidenced under cold treatment in B. napus.

关键词: DNA methylation/demethylation , MSAP , 2-DE , cold treatment , Brassica napus

Abstract: Cytosine methylation/demethylation plays pivotal roles in regulating gene expression at a genome-wide level. However, limited reports are available to reveal correlating changes of cytosine methylation and proteomic expression in Brassica napus so far. Therefore, in the present study, global cytosine methylation and proteome were analysed in B. napus after cold treatment by methylation-sensitive amplified polymorphism (MSAP) and two-dimensional protein electrophoresis technology (2-DE). The results showed that the lowered genome-wide DNA methylation status was revealed after cold treatment, and about 0.88% of discrepancy in DNA methylation was detected between the non-flowering and flowering plants after cold treatment. Moreover, the 52 significantly up-regulated proteins emerged in comparison with the 36 down-regulated proteins, as well as the 14 proteins exclusively detected in the flowering plants. Intriguingly the 8 specifically expressed proteins in the non-flowering plants disappeared in the flowering plants with cold treatment. Therefore, these present data proved that the correlating changes of cytosine methylation and proteomic expression were evidenced under cold treatment in B. napus.

Key words: DNA methylation/demethylation , MSAP , 2-DE , cold treatment , Brassica napus