Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (4): 741-748.DOI: 10.1016/S2095-3119(13)60584-5

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Abortive Process of a Novel Rapeseed Cytoplasmic Male Sterility Line Derived from Somatic Hybrids Between Brassica napus and Sinapis alba

 WANG Juan, GAO Ya-nan, KONG Yue-qin, JIANG Jin-jin, LI Ai-min, ZHANG Yong-tai   

  1. 1、Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China
    2、Jiangsu Institute of Agricultural Sciences in the Lixiahe District, Yangzhou 225009, P.R.China
  • 收稿日期:2013-02-17 出版日期:2014-04-01 发布日期:2014-04-16
  • 通讯作者: WANG You-ping, Tel: +86-514-87997303, Fax: +86-514-87991747, E-mail: wangyp@yzu.edu.cn
  • 作者简介:WANG Juan
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (31330057), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Program of International S&T Cooperation of China (1021) and the Jiangsu Province Graduate Innovation Fund (XCLX13_899), China.

Abortive Process of a Novel Rapeseed Cytoplasmic Male Sterility Line Derived from Somatic Hybrids Between Brassica napus and Sinapis alba

 WANG  Juan, GAO  Ya-nan, KONG  Yue-qin, JIANG  Jin-jin, LI  Ai-min, ZHANG  Yong-tai   

  1. 1、Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology, Yangzhou University, Yangzhou 225009, P.R.China
    2、Jiangsu Institute of Agricultural Sciences in the Lixiahe District, Yangzhou 225009, P.R.China
  • Received:2013-02-17 Online:2014-04-01 Published:2014-04-16
  • Contact: WANG You-ping, Tel: +86-514-87997303, Fax: +86-514-87991747, E-mail: wangyp@yzu.edu.cn
  • About author:WANG Juan
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31330057), the Priority Academic Program Development of Jiangsu Higher Education Institutions, the Program of International S&T Cooperation of China (1021) and the Jiangsu Province Graduate Innovation Fund (XCLX13_899), China.

摘要: Somatic hybridization is performed to obtain significant cytoplasmic male sterility (CMS) lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-1A, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-1B, and the abortive process of SaNa-1A were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-1A was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-1A aborted in the pollen mother cell (PMC) stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-1A is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther.

关键词: Brassica napus , anther abortion , cytoplasmic male sterility (CMS) , semi-thin sections , somatic hybridization

Abstract: Somatic hybridization is performed to obtain significant cytoplasmic male sterility (CMS) lines, whose CMS genes are derived either from the transfer of sterile genes from the mitochondrial genome of donor parent to the counterpart of receptor or production of new sterile genes caused by mitochondrial genome recombination of the biparent during protoplast fusion. In this study, a novel male sterile line, SaNa-1A, was obtained from the somatic hybridization between Brassica napus and Sinapis alba. The normal anther development of the maintainer line, SaNa-1B, and the abortive process of SaNa-1A were described through phenotypic observations and microtome sections. The floral organ of the sterile line SaNa-1A was sterile with a shortened filament and deflated anther. No detectable pollen grains were found on the surface of the sterile anthers. Semi-thin sections indicated that SaNa-1A aborted in the pollen mother cell (PMC) stage when vacuolization of the tapetum and PMCs began. The tapetum radically elongated and became highly vacuolated, occupying the entire locule together with the vacuolated microspores. Therefore, SaNa-1A is different from other CMS lines, such as ogu CMS, pol CMS and nap CMS as shown by the abortive process of the anther.

Key words: Brassica napus , anther abortion , cytoplasmic male sterility (CMS) , semi-thin sections , somatic hybridization