Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (6): 1042-1048.DOI: 10.1016/S2095-3119(13)60324-X

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Effects of Destruxin A on Hemocytes Morphology of Bombyx mori

 FAN Ji-qiao, CHEN Xiu-run , HU Qiong-bo   

  1. College of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China
  • 收稿日期:2012-09-11 出版日期:2013-06-01 发布日期:2013-06-09
  • 通讯作者: Correspondence HU Qiong-bo, Tel: +86-20-85280308, E-mail: hqbscau@126.com
  • 基金资助:

    This study was funded by the National 863 Program of China (2012AA101505).

Effects of Destruxin A on Hemocytes Morphology of Bombyx mori

 FAN Ji-qiao, CHEN Xiu-run , HU Qiong-bo   

  1. College of Resources and Environment, South China Agricultural University, Guangzhou 510642, P.R.China
  • Received:2012-09-11 Online:2013-06-01 Published:2013-06-09
  • Contact: Correspondence HU Qiong-bo, Tel: +86-20-85280308, E-mail: hqbscau@126.com
  • Supported by:

    This study was funded by the National 863 Program of China (2012AA101505).

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摘要： Destruxin A (DA), a kind of cyclo-hexadepsipeptide isolated from entomopathogenic fungus, Metarhizium anisopliae, is an inhibitor of insect’s immunity. But its mechanism has not been clarified yet. In this study, the effects of DA on morphologic changes of in vivo and in vitro hemocytes of silkworm, Bombyx mori, were investigated by means of inverted phase contrast microscopy (IPCM), fluorescence microscopy (FCM) and environmental scanning electron microscopy (ESEM). The results indicated that DA was cytotoxic to granulohemocytes (GR) and plasmatocytes (PL). The LC50 values of DA against in vitro GR and PL of silkworm were 68.77 and 84.11 μg mL-1, respectively. However, the hemocytes in vivo were more susceptible to DA, although at the extremely low dose of 10 μL of 12.5 μg mL-1 for each insect (i.e., 0.036 μg g-1 body weight, or approximately 0.25 μg mL-1 hemolymph), DA could induce obviously morphologic alterations of hemocytes in vivo. The results imply that there might be some factors in silkworm’s hemolymph, which influence the interaction of DA and hemocytes.

关键词: destruxin , toxicity , hemocytes , silkworm

Abstract: Destruxin A (DA), a kind of cyclo-hexadepsipeptide isolated from entomopathogenic fungus, Metarhizium anisopliae, is an inhibitor of insect’s immunity. But its mechanism has not been clarified yet. In this study, the effects of DA on morphologic changes of in vivo and in vitro hemocytes of silkworm, Bombyx mori, were investigated by means of inverted phase contrast microscopy (IPCM), fluorescence microscopy (FCM) and environmental scanning electron microscopy (ESEM). The results indicated that DA was cytotoxic to granulohemocytes (GR) and plasmatocytes (PL). The LC50 values of DA against in vitro GR and PL of silkworm were 68.77 and 84.11 μg mL-1, respectively. However, the hemocytes in vivo were more susceptible to DA, although at the extremely low dose of 10 μL of 12.5 μg mL-1 for each insect (i.e., 0.036 μg g-1 body weight, or approximately 0.25 μg mL-1 hemolymph), DA could induce obviously morphologic alterations of hemocytes in vivo. The results imply that there might be some factors in silkworm’s hemolymph, which influence the interaction of DA and hemocytes.

Key words: destruxin , toxicity , hemocytes , silkworm