Developmental Characteristics and Cinnamic Acid Resistance of Root Border Cells in Cucumber and Figleaf Gourd Seedlings

doi:10.1016/S2095-3119(13)60368-8

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (11): 2065-2073.DOI: 10.1016/S2095-3119(13)60368-8

Developmental Characteristics and Cinnamic Acid Resistance of Root Border Cells in Cucumber and Figleaf Gourd Seedlings

QIAO Yong-xu, ZHANG Yong-ping, ZHANG Hong-xin, TIAN Yong-qiang , GAO Li-hong

1.Department of Vegetable Science, China Agriculture University/Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Beijing 100193, P.R.China
2.Department of Life Science, Tangshan Normal University, Tangshan 063000, P.R.China

收稿日期:2012-09-21 出版日期:2013-11-01 发布日期:2013-12-08
通讯作者: Correspondence GAO Li-hong, Tel: +86-10-62732825, E-mail: gaolh@cau.edu.cn
基金资助:
This work was supported by the National Basic Research Program of China (973 Program, 2009CB1190002) and the National Key Technology R&D Program of China (2011BAD12B01).

Developmental Characteristics and Cinnamic Acid Resistance of Root Border Cells in Cucumber and Figleaf Gourd Seedlings

QIAO Yong-xu, ZHANG Yong-ping, ZHANG Hong-xin, TIAN Yong-qiang , GAO Li-hong

1.Department of Vegetable Science, China Agriculture University/Beijing Key Laboratory of Growth and Developmental Regulation for Protected Vegetable Crops, Beijing 100193, P.R.China
2.Department of Life Science, Tangshan Normal University, Tangshan 063000, P.R.China

Received:2012-09-21 Online:2013-11-01 Published:2013-12-08
Contact: Correspondence GAO Li-hong, Tel: +86-10-62732825, E-mail: gaolh@cau.edu.cn
Supported by:
This work was supported by the National Basic Research Program of China (973 Program, 2009CB1190002) and the National Key Technology R&D Program of China (2011BAD12B01).

摘要/Abstract

摘要： Root border cells (RBCs) originate from the root tip epidermis and surround the root apices. In this study, we evaluated the developmental characteristics and the roles of RBCs in protection of root apices of cucumber and fi gleaf gourd seedlings from CA toxicity. The formation of RBCs and the emergence of the root tip occurred almost simultaneously in root apices of cucumber and fi gleaf gourd seedlings. CA ranging from 0 to 0.25 mol L-1 inhibited root elongation and decreased root cell viability in the root tip, moreover the inhibitory effects of CA were more signifi cant in the CA-sensitive cucumber than in the CA-tolerant fi gleaf gourd. Removal of RBCs from root tips led to more severe CA induced inhibition of root elongation and decline in root cell viability. Increasing CA levels and treatment time decreased the relative viability of attached and detached RBCs. CA also induced a thicker mucilage layer surrounding attached RBCs of both species. Additionally, a signifi cantly higher relative cell viability of attached RBCs and thicker mucilage layers were observed in fi gleaf gourd. These results suggest that RBCs play an important role in protecting root tips from CA toxicity.

关键词: cinnamon acid , autotoxicity , root border cells , cell viability

Abstract: Root border cells (RBCs) originate from the root tip epidermis and surround the root apices. In this study, we evaluated the developmental characteristics and the roles of RBCs in protection of root apices of cucumber and fi gleaf gourd seedlings from CA toxicity. The formation of RBCs and the emergence of the root tip occurred almost simultaneously in root apices of cucumber and fi gleaf gourd seedlings. CA ranging from 0 to 0.25 mol L-1 inhibited root elongation and decreased root cell viability in the root tip, moreover the inhibitory effects of CA were more signifi cant in the CA-sensitive cucumber than in the CA-tolerant fi gleaf gourd. Removal of RBCs from root tips led to more severe CA induced inhibition of root elongation and decline in root cell viability. Increasing CA levels and treatment time decreased the relative viability of attached and detached RBCs. CA also induced a thicker mucilage layer surrounding attached RBCs of both species. Additionally, a signifi cantly higher relative cell viability of attached RBCs and thicker mucilage layers were observed in fi gleaf gourd. These results suggest that RBCs play an important role in protecting root tips from CA toxicity.

Key words: cinnamon acid , autotoxicity , root border cells , cell viability

QIAO Yong-xu, ZHANG Yong-ping, ZHANG Hong-xin, TIAN Yong-qiang , GAO Li-hong. Developmental Characteristics and Cinnamic Acid Resistance of Root Border Cells in Cucumber and Figleaf Gourd Seedlings[J]. Journal of Integrative Agriculture, 2013, 12(11): 2065-2073.

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Developmental Characteristics and Cinnamic Acid Resistance of Root Border Cells in Cucumber and Figleaf Gourd Seedlings

Developmental Characteristics and Cinnamic Acid Resistance of Root Border Cells in Cucumber and Figleaf Gourd Seedlings

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