A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae)

doi:10.1016/S1671-2927(00)8583

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (4): 628-638.DOI: 10.1016/S1671-2927(00)8583

A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae)

LU Qiong, ZHANG Yong-jun, CAO Guang-chun, ZHANG Li-li, LIANG Ge-mei, LU Yan-hui, WU Kong-ming, GAO Xi-wu , GUO Yu-yuan

1.College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
2.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

收稿日期:2011-04-14 出版日期:2012-04-01 发布日期:2012-04-11
通讯作者: Correspondence GUO Yu-yuan, Tel/Fax: +86-10-62894786, E-mail: yuyuanguo@hotmail.com
作者简介:LU Qiong, E-mail: luqiong19@sina.com
基金资助:
This work was supported by the National Basic Research Program of China (2012CB114104) and the National Natural Science Foundation of China (31071694 and 31171858).

A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae)

LU Qiong, ZHANG Yong-jun, CAO Guang-chun, ZHANG Li-li, LIANG Ge-mei, LU Yan-hui, WU Kong-ming, GAO Xi-wu , GUO Yu-yuan

1.College of Agriculture and Biotechnology, China Agricultural University, Beijing 100193, P.R.China
2.State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, P.R.China

Received:2011-04-14 Online:2012-04-01 Published:2012-04-11
Contact: Correspondence GUO Yu-yuan, Tel/Fax: +86-10-62894786, E-mail: yuyuanguo@hotmail.com
About author:LU Qiong, E-mail: luqiong19@sina.com
Supported by:
This work was supported by the National Basic Research Program of China (2012CB114104) and the National Natural Science Foundation of China (31071694 and 31171858).

摘要/Abstract

摘要： Cry toxins produced by Bacillus thuringiensis (Bt) are effective biological insecticides against certain insect species. In this study, bioassay results indicated that Cry1B and Cry1C were toxic to Spodoptera exigua. We also identified a cadherin-like gene in S. exigua that could enhance the toxicity of Cry1B and Cry1C. The cadherin-like gene identified from the larvae midgut tissue was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The full-length cDNA of the gene consisted of 5 220 bp encoding 1 740 amino acid with a predicted molecular mass of 196 kD. BLAST search analysis showed that the predicted amino acid sequence had a high sequence identity to the published sequences of cadherin-like proteins from other Lepidoptera insects. Spatial expression of the cadherin-like gene detected by qRT-PCR analysis revealed that the cadherin-like gene was mainly present in the gut of 4th instar larvae and during different life stages. The results suggested that the commercial development of this synergist has the potential to enhance Cry1B and Cry1C toxicity against Lepidoptera insects.

关键词: Bacillus thuringiensis, cadherin-like, Spodoptera exigua, enhancement toxicity

Abstract: Cry toxins produced by Bacillus thuringiensis (Bt) are effective biological insecticides against certain insect species. In this study, bioassay results indicated that Cry1B and Cry1C were toxic to Spodoptera exigua. We also identified a cadherin-like gene in S. exigua that could enhance the toxicity of Cry1B and Cry1C. The cadherin-like gene identified from the larvae midgut tissue was cloned by reverse transcription polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE). The full-length cDNA of the gene consisted of 5 220 bp encoding 1 740 amino acid with a predicted molecular mass of 196 kD. BLAST search analysis showed that the predicted amino acid sequence had a high sequence identity to the published sequences of cadherin-like proteins from other Lepidoptera insects. Spatial expression of the cadherin-like gene detected by qRT-PCR analysis revealed that the cadherin-like gene was mainly present in the gut of 4th instar larvae and during different life stages. The results suggested that the commercial development of this synergist has the potential to enhance Cry1B and Cry1C toxicity against Lepidoptera insects.

Key words: Bacillus thuringiensis, cadherin-like, Spodoptera exigua, enhancement toxicity

LU Qiong, ZHANG Yong-jun, CAO Guang-chun, ZHANG Li-li, LIANG Ge-mei, LU Yan-hui, WU Kong-ming, GAO Xi-wu , GUO Yu-yuan . A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae)[J]. Journal of Integrative Agriculture, 2012, 12(4): 628-638.

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A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae)

A Fragment of Cadherin-Like Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua (Lepidoptera: Noctuidae)

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