Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

doi:10.1016/S2095-3119(13)60270-1

Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (5): 825-834.DOI: 10.1016/S2095-3119(13)60270-1

Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

LIU Li-ying, DUAN Liu-sheng, ZHANG Jia-chang, MI Guo-quan, ZHANG Xiao-lan, ZHANG Zhen-xian, REN Hua-zhong

1.College of Agronomy and Bio-Technology, China Agricultural University, Beijing 100193, P.R.China
2.Horticulture Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China

收稿日期:2012-01-06 出版日期:2013-05-01 发布日期:2013-05-01
通讯作者: Correspondence REN Hua-zhong, Tel: +86-10-62731009, Fax: +86-10-62732825, E-mail: renhuazhong@cau.edu.cn
作者简介:LIU Li-ying, E-mail: liuliying35@126.com
基金资助:
This work was supported by the National Basic Research Program of China (973 Program, 2009CB11900) and the National Key Research Program of China (2008BADA6B03, 2008BADB1B05 and 2009BADB8B00).

Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

LIU Li-ying, DUAN Liu-sheng, ZHANG Jia-chang, MI Guo-quan, ZHANG Xiao-lan, ZHANG Zhen-xian, REN Hua-zhong

1.College of Agronomy and Bio-Technology, China Agricultural University, Beijing 100193, P.R.China
2.Horticulture Institute, Henan Academy of Agricultural Sciences, Zhengzhou 450002, P.R.China

Received:2012-01-06 Online:2013-05-01 Published:2013-05-01
Contact: Correspondence REN Hua-zhong, Tel: +86-10-62731009, Fax: +86-10-62732825, E-mail: renhuazhong@cau.edu.cn
About author:LIU Li-ying, E-mail: liuliying35@126.com
Supported by:
This work was supported by the National Basic Research Program of China (973 Program, 2009CB11900) and the National Key Research Program of China (2008BADA6B03, 2008BADB1B05 and 2009BADB8B00).

摘要/Abstract

摘要： Low temperature and high salinity are the major abiotic stresses that restrict cucumber growth and production, breeding materials with multiple abiotic resistance are in greatly need. Here we investigated the effect of introducing the LOS5 gene, a key regulator of ABA biosynthesis in Arabidopsis thaliana, under the stress-responsive RD29A promoter into cucumber (Cucumis sativus L. cv. S516). We found that T1 RD29A-LOS5 transgenic lines have enhanced tolerance to cold and salt stresses. Specifically, transgenic lines exhibited dwarf phenotypes with reduced leaf number, shorter internode, decreased length of the biggest leaf, fewer female flowers, shorter fruit neck and lower vitamin C (Vc). The increased cold tolerance can be reflected from the significantly decreased cold index, the reduced electrolyte leakage index and the MDA content upon cold treatment as compared to those in the control. This may result from the accumulation of internal ABA, soluble sugars and proline, and the enhanced activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the transgenic lines. Under salt treatment, the transgenic lines exhibited increased germination index, vigor index, more lateral roots and increased root fresh weight. Moreover, RD29A-LOS5 transgenic plants displayed quicker responses in salt stress than that in low-temperature stress.

关键词: ABA , low-temperature stress , LOS5 , transgenic plant , cucumber , salt stress

Abstract: Low temperature and high salinity are the major abiotic stresses that restrict cucumber growth and production, breeding materials with multiple abiotic resistance are in greatly need. Here we investigated the effect of introducing the LOS5 gene, a key regulator of ABA biosynthesis in Arabidopsis thaliana, under the stress-responsive RD29A promoter into cucumber (Cucumis sativus L. cv. S516). We found that T1 RD29A-LOS5 transgenic lines have enhanced tolerance to cold and salt stresses. Specifically, transgenic lines exhibited dwarf phenotypes with reduced leaf number, shorter internode, decreased length of the biggest leaf, fewer female flowers, shorter fruit neck and lower vitamin C (Vc). The increased cold tolerance can be reflected from the significantly decreased cold index, the reduced electrolyte leakage index and the MDA content upon cold treatment as compared to those in the control. This may result from the accumulation of internal ABA, soluble sugars and proline, and the enhanced activities of protective enzymes superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT) in the transgenic lines. Under salt treatment, the transgenic lines exhibited increased germination index, vigor index, more lateral roots and increased root fresh weight. Moreover, RD29A-LOS5 transgenic plants displayed quicker responses in salt stress than that in low-temperature stress.

Key words: ABA , low-temperature stress , LOS5 , transgenic plant , cucumber , salt stress

LIU Li-ying, DUAN Liu-sheng, ZHANG Jia-chang, MI Guo-quan, ZHANG Xiao-lan, ZHANG Zhen-xian, REN Hua-zhong. Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber[J]. Journal of Integrative Agriculture, 2013, 12(5): 825-834.

参考文献

[1]Aswath C R, Kim S H, Mo SY, Kim D H. 2005. Transgenicplants of creeping bent grass harboring the stressinducible gene, 9-cis-epoxycarotenoid dioxygenase,are highly tolerant to drought and NaCl stress. PlantGrowth Regulation, 47, 129-139

[2]Boyer R F. 1986. Modern Experimental Biochemistry.Addison-Wesley, New York. pp. 515-521

[3]Bradford M M. 1976. A rapid and sensitive method for thequantitation of microgram quantities of protein utilizing the principle of protein-dye binding. AnalyticalBiochemistry, 72, 248-254

[4]Bray E A. 1993. Molecular responses to water deficit. PlantPhysiology, 103, 1035-1040

[5]van Breusegem F, Dat J F. 2006. Reactive oxygen speciesin plant cell death. Plant Physiology, 141, 384-390

[6]Cakmak I, Strbac D, Marschner H. 1993. Activities ofhydrogen peroxide-scavenging enzymes in germinatingwheat seeds. The Journals of Experimental Botany,44, 127-132

[7]Dhindsa D. 1980. Immunological Aspects of Infertility &Fertility Regulation. Elsevier Science Publising Co.,New York.Esquinas-Alcazar J T, Gulick P J. 1983. Gegetic Resourcesof Cucurbitaceae. International Board for Plant GeneticResources Secretariat, Rome, Italy. pp. 1-101

[8]Etehadnia M, Waterer D, Jong H D, Tanino K K. 2008.Scion and Rootstock effects on ABA-mediated plantgrowth regulation and salt tolerance of acclimated andunacclimated potato genotypes. Journal of PlantGrowth Regulation, 27, 125-140

[9]Fumio H, Takuya I, Minoru O, Keishiro W. 2000. Oscillationand regulation of proline content by P5CS and ProDHgene expressions in the light/dark cycles in Arabidopsisthaliana L. Plant Cell Physiology, 41, 1096-1101

[10]Heath R L, Parker L. 1968. Photoperoxidation in isolatedchloroplasts. I. Kinetics and stoichiometry of fatty acidperoxidation. Archives Biochemistry and Biophysics,125, 189-198

[11]Huang J, Zhu J K. 2004. Plant response to stress: abscisicacid and water stress. In: Robert Goodman M, ed.,Encyclopedia of Plant and Crop Science. CRC Press,USA. pp. 1-3

[12]Ingram, Bartel D. 1996. The molecular basis of dehydrationtolerance in plants. Annual Review of Plant Physiologyand Plant Molecular Biology, 47, 377-403

[13]Iuchi S, Kobayashi M, Taji T, Naramoto M, Seki M, Kato T,Tabata S, Kakubari Y, Yamaguchi-Shinozaki K, ShinozakiK. 2001. Regulation of drought tolerance by genemanipulation of 9-cis-epoxycarotenoid dioxygenase,a key enzyme in abscisic acid biosynthesis inArabidopsis. Plant Journal, 27, 325-333

[14]Kasuga M, Miura S, Shinozaki K, Yamaguchi-Shinozaki K.2004. A combination of the Arabidopsis DREB1A geneand stress-inducible rd29A promoter improveddrought- and low-temperature stress tolerance intobacco by gene transfer. Plant Cell Physiology, 45,346-350

[15]Koornneef M, Jorna M L, Brinkhorst-van der Swan D L C,Karssen C M. 1982. The isolation of abscisic aciddeficientmutants by selection of induced revertants innon-germinating gibberellin sensitive lines ofArabidopsis thaliana. Theoretical and AppliedGenetics, 61, 385-393

[16]McCourt P. 1999. Genetic analysis of hormone signaling.Annual Review of Plant Physiology and PlantMolecular Biology, 50, 219-243

[17]Murray M B, Cape J N, Fowler D. 1989. Quantification offrost damage in plant tissues by rates of electrolyteleakage. New Phytologist, 113, 307-311

[18]Pitzschke A, Hirt H. 2006. Mitogen-activated protein kinasesand reactive oxygen species signaling in plants. PlantPhysiology, 141, 351-356

[19]Qin X, Zeevaart J A D. 2002. Overexpression of a 9-cisepoxycarotenoiddioxygenase gene in Nicotianaplumbaginifolia increases abscisic acid and phaseicacid levels and enhances drought tolerance. PlantPhysiology, 128, 544-551

[20]Rock C D. 2000. Pathways to abscisic acid-regulated geneexpression. New Phytologist, 148, 357-396

[21]Saghai-Maroof M A, Soliman K M, Jorgensen R A, AllardR W. 1984. Ribosomal DNA spacer lengthpolymorphisms in barely: mendelian inheritance,chromosomal location, and population dynamics.Proceedings of the National Academy of Sciences ofthe United States of America, 81, 8014-8018

[22]Semeniuk P, Moline H E, Abbott J A. 1986. A comparison ofthe effects of ABA and an antitranspirant on chillinginjury of coleus, cucumbers, and Dieffenbachia. Journalof Americcan Society for Horticultural Science, 111,866-868

[23]Singla-Pareek S L, Pareek A, Sopory S K. 2007. Transgenicplants for dry and saline environments. In: Jenks M A,Hasegawa P M, Jain S M, eds., Advances in MolecularBreeding Toward Drought and Salt Tolerant Crops.Springer, Germany. pp. 501-530

[24]Tabei Y, Kitade S, Nishizawa Y, Kikuchi N, Kayano T, HibiT, Akutsu K. 1998. Transgenic cucumber plantsharboring a rice chitinase gene exhibit enhancedresistance to gray mold (Botrytis cinerea). Plant CellReports, 17, 159-164

[25]Thomashow M F. 1999. Plant cold acclimation, freezingtolerance genes and regulatory mechanisms. AnnualReview of Plant Physiology and Plant MolecularBiology, 50, 571-599

[26]Thomashow M F. 2001. So what’s new in the field of plantcold acclimation? Lots! Plant Physiology, 125, 89-93

[27]Thompson A J, Jackson A C, Symonds R C, Mulholland BJ, Dadswell A R, Blake P S, Burbidge A, Taylor I B. 2000.Ectopic expression of a tomato 9-cis-epoxycarotenoiddioxygenase gene causes over-production of abscisicacid. Plant Journal, 23, 363-374

[28]Thompson A J, Andrews J, Mulholland B J, McKee J M T,Hilton H W, Horridge J S, Farquhar G D, Smeeton R C,Smillie I R A, Black C R, et al. 2007. Overproduction ofabscisic acid in tomato increases transpirationefficiency and root hydraulic conductivity andinfluences leaf expansion. Plant Physiology, 143, 1905-1917

[29]Troll W, Lindsley J. 1955. A photometric method for thedetermination of praline. The Journal of BiologicalChemistry, 215, 655-660

[30]Wang F Z, Wang Q B, Kwon SY, Kwak S S, Su W A. 2005.Enhanced drought tolerance of transgenic rice plantsexpressing a pea manganese superoxide dismutase.Journal of Plant Physiology, 162, 465-472

[31]Xin Z, Browse J. 1998. Eskimo1 mutants of Arabidopsisare constitutively freezing-tolerant. The Proceedingsof the National Academy of Science of the United Statesof America, 95, 7799-7804

[32]Xiong L, Ishitani M, Lee H, Zhu J K. 2001. The ArabidopsisLOS5/ABA3 locus encodes a molybdenum cofactorsulfuase and moldulates cold stress- and osmoticstress-responsive gene expression. The Plant Cell, 13,2063-2083

[33]Xiong L, Schumaker K S, Zhu J K. 2002. Cell signalingduring cold, drought, and salt stress. The Plant Cell,Suppl., 165-183

[34]Yem W, Willis A J. 1954. The estimation of carbohydratesin plant extraction by anthrone. Biochemistry Journal,57, 508.

Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

Arabidopsis LOS5 Gene Enhances Chilling and Salt Stress Tolerance in Cucumber

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	MIAO Yan-xiu, WANG Xiao-zhuo, GAO Li-hong, CHEN Qing-yun, QU Mei. Blue light is more essential than red light for maintaining the activities of photosystem II and I and photosynthetic electron transport capacity in cucumber leaves[J]. Journal of Integrative Agriculture, 2016, 15(1): 87-100.
[2]	MAO Lian-gang, WANG Qiu-xia, YAN Dong-dong, LIU Peng-fei, SHEN Jin, FANG Wen-sheng, HU Xiao-mei, LI Yuan, OUYANG Can-bin, GUO Mei-xia, CAO Ao-cheng. Application of the combination of 1,3-dichloropropene and dimethyl disulfide by soil injection or chemigation: effects against soilborne pests in cucumber in China[J]. Journal of Integrative Agriculture, 2016, 15(1): 145-152.
[3]	CHENG Chun-zhen, YANG Jia-wei, YAN Hu-bin, BEI Xue-jun, ZHANG Yong-yan, LU Zhi-ming, ZHONG Guang-yan. Expressing p20 hairpin RNA of Citrus tristeza virus confers Citrus aurantium with tolerance/resistance against stem pitting and seedling yellow CTV strains[J]. Journal of Integrative Agriculture, 2015, 14(9): 1767-1777.
[4]	ZHAO Lei, ZHANG Ya-qing. Effects of phosphate solubilization and phytohormone production of Trichoderma asperellum Q1 on promoting cucumber growth under salt stress[J]. Journal of Integrative Agriculture, 2015, 14(8): 1588-1597.
[5]	MA Ya-nan, CHEN Ming, XU Dong-bei, FANG Guang-ning, WANG Er-hui, GAO Shi-qing, XU Zhao-shi, LI Lian-cheng, ZHANG Xiao-hong, MIN Dong-hong, MA You-zhi. G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis[J]. Journal of Integrative Agriculture, 2015, 14(2): 314-325.
[6]	XIE Jun-yu, XU Ming-gang, Qiangjiu Ciren, YANG Yang, ZHANG Shu-lan, SUN Ben-hua, YANG Xue-yun. Soil aggregation and aggregate associated organic carbon and total nitrogen under long-term contrasting soil management regimes in loess soil[J]. Journal of Integrative Agriculture, 2015, 14(12): 2405-2416.
[7]	QIANG Xiao-jing, YU Guo-hong, JIANG Lin-lin, SUN Lin-lin, ZHANG Shu-hui, LI Wei, CHENG Xian-guo. Thellungiella halophila ThPIP1 gene enhances the tolerance of the transgenic rice to salt stress[J]. Journal of Integrative Agriculture, 2015, 14(10): 1911-1922.
[8]	YE Shu-e, LI Fang, LI Xian-bi, HONG Qi-bin, ZHAI Yun-lan, HU Ming-yu, WEI Ting, DENG Sha-sha, PEI Yan, LUO Ming. Over-expression of GhDWF4 gene improved tomato fruit quality and accelerated fruit ripening[J]. Journal of Integrative Agriculture, 2015, 14(10): 1980-1991.
[9]	WANG Li-li, ZHANG Peng, QIN Zhi-wei , ZHOU Xiu-yan. Proteomic Analysis of Fruit Bending in Cucumber (Cucumis sativus L.)[J]. Journal of Integrative Agriculture, 2014, 13(5): 963-974.
[10]	SUN Jian-lei, SUI Xiao-lei, HUANG Hong-yu, WANG Shao-hui, WEI Yu-xia , ZHANG Zhenxian. Low Light Stress Down-Regulated Rubisco Gene Expression and Photosynthetic Capacity During Cucumber (Cucumis sativus L.) Leaf Development[J]. Journal of Integrative Agriculture, 2014, 13(5): 997-1007.
[11]	WEI Shi-wei, ZHANG Fu-rong, ZHANG Yi-dong, WANG Li-min, CHEN Jia-bei , HUANG Danfeng. Comparative Analysis of Gene Expression in Two Muskmelon Cultivars (Cucumis melo L.) Under Salt Stress[J]. Journal of Integrative Agriculture, 2014, 13(10): 2132-2140.
[12]	ZHANG Xiao-ming, YANG Nian-wan, WAN Fang-hao , Gabor L L?vei. Density and Seasonal Dynamics of Bemisia tabaci (Gennadius) Mediterranean on Common Crops and Weeds Around Cotton Fields in Northern China[J]. Journal of Integrative Agriculture, 2014, 13(10): 2211-2220.
[13]	QIN Hua, ZHOU Shuang , ZHANG Yi-zheng. Characterization and Expression Analysis of Starch Branching Enzymes in Sweet Potato[J]. Journal of Integrative Agriculture, 2013, 12(9): 1530-1539.
[14]	QIAN Chun-lu, MI Hong-bo, ZHAO Yu-ying, HE Zhi-ping , MAO Lin-chun. Effect of Maturity Stage on the Gene Expression of Antioxidative Enzymes in Cucumber (Cucumis sativus L.) Fruits Under Chilling Stress[J]. Journal of Integrative Agriculture, 2013, 12(8): 1495-1500.
[15]	ZHANG Zi-kun, LI Hua, HE Hong-jun , LIU Shi-qi. Grafting Raises the Cu Tolerance of Cucumber Through Protecting Roots Against Oxidative Stress Induced by Cu Stress[J]. Journal of Integrative Agriculture, 2013, 12(5): 815-824.