Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (5): 997-1007.DOI: 10.1016/S2095-3119(13)60670-X

• 论文 • 上一篇    下一篇

Low Light Stress Down-Regulated Rubisco Gene Expression and Photosynthetic Capacity During Cucumber (Cucumis sativus L.) Leaf Development

 SUN Jian-lei, SUI Xiao-lei, HUANG Hong-yu, WANG Shao-hui, WEI Yu-xia , ZHANG Zhenxian   

  1. 1、Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops/College of Agriculture and Biotechnology,
    China Agricultural University, Beijing 100193, P.R.China
    2、Plant Science Department, Beijing University of Agriculture, Beijing 102206, P.R.China
  • 收稿日期:2013-02-28 出版日期:2014-05-01 发布日期:2014-05-06
  • 通讯作者: ZHANG Zhen-xian, Tel: +86-10-62731952, Fax: +86-10-62731952, E-mail: zhangzx@cau.edu.cn
  • 作者简介:SUN Jian-lei, E-mail: sunjianlei06@hotmail.com; SUI Xiao-lei, E-mail: sui-office@cau.edu.cn
  • 基金资助:

    This work was supported by China Agriculture Research System (CARS-25), the National Basic Research Program of China (973 Program, 2009CB11900) and the National Key Technologies R&D Program of China (2011BAD12B01).

Low Light Stress Down-Regulated Rubisco Gene Expression and Photosynthetic Capacity During Cucumber (Cucumis sativus L.) Leaf Development

 SUN Jian-lei, SUI Xiao-lei, HUANG Hong-yu, WANG Shao-hui, WEI Yu-xia , ZHANG Zhenxian   

  1. 1、Beijing Key Laboratory of Growth and Development Regulation for Protected Vegetable Crops/College of Agriculture and Biotechnology,
    China Agricultural University, Beijing 100193, P.R.China
    2、Plant Science Department, Beijing University of Agriculture, Beijing 102206, P.R.China
  • Received:2013-02-28 Online:2014-05-01 Published:2014-05-06
  • Contact: ZHANG Zhen-xian, Tel: +86-10-62731952, Fax: +86-10-62731952, E-mail: zhangzx@cau.edu.cn
  • About author:SUN Jian-lei, E-mail: sunjianlei06@hotmail.com; SUI Xiao-lei, E-mail: sui-office@cau.edu.cn
  • Supported by:

    This work was supported by China Agriculture Research System (CARS-25), the National Basic Research Program of China (973 Program, 2009CB11900) and the National Key Technologies R&D Program of China (2011BAD12B01).

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摘要： Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber (Cucumis sativus L.) in solar greenhouses in northern China. Here, two genotypes of cucumber (Deltastar and Jinyan 2) are used to determine the effect of low light stress on Rubisco expression and photosynthesis of leaves from emergence to senescence. During leaf development, the net photosynthetic rate (PN), stomatal conductance (gs), Rubisco initial activity and activation state, transcript levels of rbcL and rbcS, and the abundance of rbcL and rbcS DNA in these two genotypes increase rapidly to reach maximum in 10-20 d, and then decrease gradually. Meanwhile, the actual photosystem II efficiency (ФPSII) of cucumber leaves slowly increased in the early leaf developing stages, but it declined quickly in leaf senescent stages, accompanied by an increased non-photochemical quenching (NPQ). Moreover, PN, gs, initial Rubisco activity, and abundance of protein, mRNA and DNA of Rubisco subunits of leaves grown under 100 μmol m-2 s-1 are lower, and require more time to reach their maxima than those grown under 600 μmol m-2 s-1 during leaf development. All these results suggest that lower photosynthetic capacity of cucumber leaves from emergence to senescence under low light stress is probably due to down-regulated Rubisco gene expression in transcript and protein levels, and decreased initial and total activity as well as activation state of Rubisco. Deltastar performs better than Jinyan 2 under low light stress.

关键词: cucumber , photosynthetic capacity , Rubisco , rbcL , rbcS , low light

Abstract: Low light stress is one of the most important factors affecting photosynthesis and growth in winter production of cucumber (Cucumis sativus L.) in solar greenhouses in northern China. Here, two genotypes of cucumber (Deltastar and Jinyan 2) are used to determine the effect of low light stress on Rubisco expression and photosynthesis of leaves from emergence to senescence. During leaf development, the net photosynthetic rate (PN), stomatal conductance (gs), Rubisco initial activity and activation state, transcript levels of rbcL and rbcS, and the abundance of rbcL and rbcS DNA in these two genotypes increase rapidly to reach maximum in 10-20 d, and then decrease gradually. Meanwhile, the actual photosystem II efficiency (ФPSII) of cucumber leaves slowly increased in the early leaf developing stages, but it declined quickly in leaf senescent stages, accompanied by an increased non-photochemical quenching (NPQ). Moreover, PN, gs, initial Rubisco activity, and abundance of protein, mRNA and DNA of Rubisco subunits of leaves grown under 100 μmol m-2 s-1 are lower, and require more time to reach their maxima than those grown under 600 μmol m-2 s-1 during leaf development. All these results suggest that lower photosynthetic capacity of cucumber leaves from emergence to senescence under low light stress is probably due to down-regulated Rubisco gene expression in transcript and protein levels, and decreased initial and total activity as well as activation state of Rubisco. Deltastar performs better than Jinyan 2 under low light stress.

Key words: cucumber , photosynthetic capacity , Rubisco , rbcL , rbcS , low light