SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum

doi:10.1016/S2095-3119(14)60859-5

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (2): 326-336.DOI: 10.1016/S2095-3119(14)60859-5

SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum

SUN Xiao-chun, GAO Yong-feng, ZHANG Ning, LI Hui-rong, YANG Shu-zhang, LIU Yong-sheng

1、Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment/State Key Laboratory of Hydraulics and Mountain
River Engineering/College of Life Science, Sichuan University, Chengdu 610064, P.R.China
2、School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, P.R.China

收稿日期:2014-03-31 出版日期:2015-02-01 发布日期:2015-02-11
通讯作者: LIU Yong-sheng, Fax: +86-551-62919399;E-mail: liuyongsheng1122@hfut.edu.cn
作者简介:S U N X i a o - c h u n , E - m a i l : s u n x i a o c h u n 0 8 @ 1 6 3 . c o m ;
基金资助:
This work was supported by the National Science Fund for Distinguished Young Scholars, China (30825030), the National Natural Science Foundation of China (31171179, 90717110), the National Basic Research Program of China (973, 2011CB100401) and the Advanced Program of Doctoral Fund of Ministry of Education of China (20110181130009).

SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum

SUN Xiao-chun, GAO Yong-feng, ZHANG Ning, LI Hui-rong, YANG Shu-zhang, LIU Yong-sheng

1、Ministry of Education Key Laboratory for Bio-Resource and Eco-Environment/State Key Laboratory of Hydraulics and Mountain
River Engineering/College of Life Science, Sichuan University, Chengdu 610064, P.R.China
2、School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei 230009, P.R.China

Received:2014-03-31 Online:2015-02-01 Published:2015-02-11
Contact: LIU Yong-sheng, Fax: +86-551-62919399;E-mail: liuyongsheng1122@hfut.edu.cn
About author:S U N X i a o - c h u n , E - m a i l : s u n x i a o c h u n 0 8 @ 1 6 3 . c o m ;
Supported by:
This work was supported by the National Science Fund for Distinguished Young Scholars, China (30825030), the National Natural Science Foundation of China (31171179, 90717110), the National Basic Research Program of China (973, 2011CB100401) and the Advanced Program of Doctoral Fund of Ministry of Education of China (20110181130009).

摘要/Abstract

摘要： SOM encodes a nucleus-localized CCCH-type zinc finger protein and negatively regulates seed germination in Arabidopsis thaliana. We have previously demonstrated that ectopic expression of SlABI3, an important transcription factor in abscisic acid (ABA) signaling pathway, resulted in alteration of SlSOM expression patterns in both leaf and seed of tomato. In this study, we aimed to elucidate the function of tomato SlSOM in regarding to seed germination and seedling development. Here, we constructed SlSOM over-expression vector pBI121-SOM driven by CaMV 35S promoter, and the recombinant plasmid was incorporated into wild-type tomato by the method of Agrobacterium tumefaciens-mediated transformation. The result showed that over-expression of SlSOM conferred enhanced responses to exogenous ABA application during seed germination and seedling development. In addition, ectopic expression of SlSOM resulted in the alteration of expression level of ABA/GA (gibberellins) metabolic genes, such as SlABA1, SlCYP707A1, SlGA3ox2, and SlGA2ox4, in both leaf and seed. The ABA anabolic gene SlABA1 and the GA catabolic gene SlGA2ox4 were up-regulated while the ABA catabolic gene SlCYP707A1 and the GA anabolic gene SlGA3ox2 were down-regulated. Compared to wild type, the expression level of SlABI5 was increased by about 40–50% in transgenic seeds while adding exogenous ABA treatment. These results support the notion that SlSOM inhibits seed germination by regulating ABA/GA metabolic genes and SlABI5 expression in Solanum lycopersicum.

关键词: tomato , SOM , seed germination , abscisic acid

Abstract: SOM encodes a nucleus-localized CCCH-type zinc finger protein and negatively regulates seed germination in Arabidopsis thaliana. We have previously demonstrated that ectopic expression of SlABI3, an important transcription factor in abscisic acid (ABA) signaling pathway, resulted in alteration of SlSOM expression patterns in both leaf and seed of tomato. In this study, we aimed to elucidate the function of tomato SlSOM in regarding to seed germination and seedling development. Here, we constructed SlSOM over-expression vector pBI121-SOM driven by CaMV 35S promoter, and the recombinant plasmid was incorporated into wild-type tomato by the method of Agrobacterium tumefaciens-mediated transformation. The result showed that over-expression of SlSOM conferred enhanced responses to exogenous ABA application during seed germination and seedling development. In addition, ectopic expression of SlSOM resulted in the alteration of expression level of ABA/GA (gibberellins) metabolic genes, such as SlABA1, SlCYP707A1, SlGA3ox2, and SlGA2ox4, in both leaf and seed. The ABA anabolic gene SlABA1 and the GA catabolic gene SlGA2ox4 were up-regulated while the ABA catabolic gene SlCYP707A1 and the GA anabolic gene SlGA3ox2 were down-regulated. Compared to wild type, the expression level of SlABI5 was increased by about 40–50% in transgenic seeds while adding exogenous ABA treatment. These results support the notion that SlSOM inhibits seed germination by regulating ABA/GA metabolic genes and SlABI5 expression in Solanum lycopersicum.

Key words: tomato , SOM , seed germination , abscisic acid

SUN Xiao-chun, GAO Yong-feng, ZHANG Ning, LI Hui-rong, YANG Shu-zhang, LIU Yong-sheng. SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum[J]. Journal of Integrative Agriculture, 2015, 14(2): 326-336.

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SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum

SlSOM inhibits seed germination by regulating the expression of ABA/GA metabolic genes and SlABI5 in Solanum lycopersicum

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