G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis

doi:10.1016/S2095-3119(14)60777-2

Journal of Integrative Agriculture ›› 2015, Vol. 14 ›› Issue (2): 314-325.DOI: 10.1016/S2095-3119(14)60777-2

G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis

MA Ya-nan, CHEN Ming, XU Dong-bei, FANG Guang-ning, WANG Er-hui, GAO Shi-qing, XU Zhao-shi, LI Lian-cheng, ZHANG Xiao-hong, MIN Dong-hong, MA You-zhi

1、College of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100,
P.R.China
2、Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and
Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing
100081, P.R.China
3、Beijing Hybrid Wheat Engineering and Technology Research Center, Beijing 100097, P.R.China

收稿日期:2014-01-27 出版日期:2015-02-01 发布日期:2015-02-11
通讯作者: MIN Dong-hong, E-mail: mdh2493@126.com;MA You-zhi, E-mail: mayouzhi@caas.cn
作者简介:These authors contributed equally to this study.
基金资助:
The work was funded in part by the National Key Project for Research on Transgenic Biology (2013ZX08002-002) and the National Natural Science Foundation of China (31201200).

G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis

MA Ya-nan, CHEN Ming, XU Dong-bei, FANG Guang-ning, WANG Er-hui, GAO Shi-qing, XU Zhao-shi, LI Lian-cheng, ZHANG Xiao-hong, MIN Dong-hong, MA You-zhi

1、College of Agronomy, Northwest A&F University/State Key Laboratory of Arid Region Crop Adversity Biology, Yangling 712100,
P.R.China
2、Institute of Crop Sciences, Chinese Academy of Agricultural Sciences/National Key Facility for Crop Gene Resources and
Genetic Improvement/Key Laboratory of Biology and Genetic Improvement of Triticeae Crops, Ministry of Agriculture, Beijing
100081, P.R.China
3、Beijing Hybrid Wheat Engineering and Technology Research Center, Beijing 100097, P.R.China

Received:2014-01-27 Online:2015-02-01 Published:2015-02-11
Contact: MIN Dong-hong, E-mail: mdh2493@126.com;MA You-zhi, E-mail: mayouzhi@caas.cn
About author:These authors contributed equally to this study.
Supported by:
The work was funded in part by the National Key Project for Research on Transgenic Biology (2013ZX08002-002) and the National Natural Science Foundation of China (31201200).

摘要/Abstract

摘要： The heterotrimeric GTP-binding proteins (G-proteins) in eukaryotes consisted of α, β and γ subunits and are important in molecular signaling by interacting with G-protein-coupled receptors (GPCR), on which to transduce signaling into the cytoplast through appropriate downstream effectors. However, downstream effectors regulated by the G-proteins in plants are currently not well defined. In this study, the transcripts of AGB1, a G protein β subunit gene in Arabidopsis were found to be down-regulated by cold and heat, but up-regulated by high salt stress treatment. AGB1 mutant (agb1-2) was more sensitive to high salt stress than wild-type (WT). Compared with WT, the cotyledon greening rates, fresh weight, root length, seedling germination rates and survival rates decreased more rapidly in agb1-2 along with increasing concentrations of NaCl in normal (MS) medium. Physiological characteristic analysis showed that compared to WT, the contents of chlorophyll, relative proline accumulation and peroxidase (POD) were reduced, whereas the malonaldehyde (MDA) content and concentration ratio of Na+/K+ were increased in agb1-2 under salt stress condition. Further studies on the expression of several stress inducible genes associated with above physiological processes were investigated, and the results revealed that the expressions of genes related to proline biosynthesis, oxidative stress response, Na+ homeostasis, stress- and ABAresponses were lower in agb1-2 than in WT, suggesting that those genes are possible downstream genes of AGB1 and that their changed expression plays an important role in determining phenotypic and physiologic traits in agb1-2. Taken together, these findings indicate that AGB1 positively regulates salt tolerance in Arabidopsis through its modulation of genes transcription related to proline biosynthesis, oxidative stress, ion homeostasis, stress- and ABA-responses.

关键词: Arabidopsis , heterotrimeric G-protein &beta, subunit , physiological processes , salt stress tolerance

Abstract: The heterotrimeric GTP-binding proteins (G-proteins) in eukaryotes consisted of α, β and γ subunits and are important in molecular signaling by interacting with G-protein-coupled receptors (GPCR), on which to transduce signaling into the cytoplast through appropriate downstream effectors. However, downstream effectors regulated by the G-proteins in plants are currently not well defined. In this study, the transcripts of AGB1, a G protein β subunit gene in Arabidopsis were found to be down-regulated by cold and heat, but up-regulated by high salt stress treatment. AGB1 mutant (agb1-2) was more sensitive to high salt stress than wild-type (WT). Compared with WT, the cotyledon greening rates, fresh weight, root length, seedling germination rates and survival rates decreased more rapidly in agb1-2 along with increasing concentrations of NaCl in normal (MS) medium. Physiological characteristic analysis showed that compared to WT, the contents of chlorophyll, relative proline accumulation and peroxidase (POD) were reduced, whereas the malonaldehyde (MDA) content and concentration ratio of Na+/K+ were increased in agb1-2 under salt stress condition. Further studies on the expression of several stress inducible genes associated with above physiological processes were investigated, and the results revealed that the expressions of genes related to proline biosynthesis, oxidative stress response, Na+ homeostasis, stress- and ABAresponses were lower in agb1-2 than in WT, suggesting that those genes are possible downstream genes of AGB1 and that their changed expression plays an important role in determining phenotypic and physiologic traits in agb1-2. Taken together, these findings indicate that AGB1 positively regulates salt tolerance in Arabidopsis through its modulation of genes transcription related to proline biosynthesis, oxidative stress, ion homeostasis, stress- and ABA-responses.

Key words: Arabidopsis , heterotrimeric G-protein &beta, subunit , physiological processes , salt stress tolerance

MA Ya-nan, CHEN Ming, XU Dong-bei, FANG Guang-ning, WANG Er-hui, GAO Shi-qing, XU Zhao-shi, LI Lian-cheng, ZHANG Xiao-hong, MIN Dong-hong, MA You-zhi. G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis[J]. Journal of Integrative Agriculture, 2015, 14(2): 314-325.

参考文献

Aono M, Kubo A, Saji H, Tanaka K, Kondo N. 1993. Enhancedtolerance to photooxidative stress of transgenic Nicotianatabacum with high chloroplastic glutathione reductaseactivity. Plant and Cell Physiology, 34, 129-135

Apel K, Hirt H. 2004. Reactive oxygen species: Metabolism,oxidative stress, and signal transduction. Annual Reviewof Plant Biology, 55, 373-399

Apse M P, Aharon G S, Snedden W A, Blumwald E. 1999. Salttolerance conferred by overexpression of a vacuolar Na+/H+antiport in Arabidopsis. Science, 285, 1256-1258

Blaha G, Stelzl U, Spahn C M, Agrawal R K, Frank J, NiehausK H. 2000. Preparation of functional ribosomal complexesand effect of buffer conditions on tRNA positions observedby cryoelectron microscopy. Methods in Enzymology, 317,292-309

Bohnert H J, Jensen R G. 1996. Strategies for engineeringwater-stress tolerance in plants. Trends in Biotechnology,14, 89-97

Booker F L, Burkey K O, Overmyer K, Jones A M. 2004.Differential responses of G-protein Arabidopsis thalianamutants to ozone. New Phytologist, 162, 633-641

Chakravorty D, Trusov Y, Zhang W, Acharya B R, Sheahan M B,McCurdy D W, Assmann S M, Botella J R. 2011. An atypicalheterotrimeric G-protein γ-subunit is involved in guard cellK+-channel regulation and morphological development inArabidopsis thaliana. The Plant Journal, 67, 840-851

Chen J G, Gao Y, Jones A M. 2006. Differential roles ofArabidopsis heterotrimeric G-protein subunits in modulatingcell division in roots. Plant Physiology, 141, 887-897

Chen J G, Pandey S, Huang J, Alonso J M, Ecker J R, AssmannS M, Jones A M. 2004. GCR1 can act independently ofheterotrimeric G-protein in response to brassinosteroidsand gibberellins in Arabidopsis seed germination. PlantPhysiology, 135, 907-915

Clipson N, Lachno D, Flowers T. 1988. Salt tolerance inthe halophyte Suaeda maritima L. Dum.: Abscisic acidconcentrations in response to constant and altered salinity.Journal of Experimental Botany, 39, 1381-1388

Cuin T A, Miller A J, Laurie S A, Leigh R A. 2003. Potassiumactivities in cell compartments of salt-grown barley leaves.Journal of Experimental Botany, 54, 657-661

Daudi A, Cheng Z, O’Brien JA, Mammarella N, Khan S, AusubelF M, Bolwell G P. 2012. The apoplastic oxidative burstperoxidase in Arabidopsis is a major component of patterntriggeredimmunity. The Plant Cell, 24, 275-287

Delgado-Cerezo M, Sánchez-Rodríguez C, Escudero V,Miedes E, Fernández P V, Jordá L, Hernández-Blanco C,Sánchez-Vallet A, Bednarek P, Schulze-Lefert P, SomervilleS, Estevez J M, Persson S, Molina A. 2012. Arabidopsisheterotrimeric G-protein regulates cell wall defense andresistance to necrotrophic fungi. Molecular Plant, 5, 98-114

Ding L, Zhu J K. 1997. Reduced Na+ uptake in the NaClhypersensitivesos1 mutant of Arabidopsis thaliana. PlantPhysiology, 113, 795-799

Dubouzet J G, Sakuma Y, Ito Y, Kasuga M, Dubouzet E G,Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K.2003. OsDREB genes in rice, Oryza sativa L., encodetranscription activators that function in drought-, high-saltandcold-responsive gene expression. The Plant Journal,33, 751-763

Friedman E J, Wang H X, Jiang K, Perovic I, Deshpande A,Pochapsky T C, Temple B R, Hicks S N, Harden T K,Jones A M. 2011. ACIREDUCTONE DIOXYGENASE 1(ARD1) is an effector of the heterotrimeric G protein βsubunit in Arabidopsis. Journal of Biological Chemistry,286, 30107-30118

Gorsuch P A, Sargeant A W, Penfield S D, Quick W P, Atkin OK. 2010. Systemic low temperature signaling in Arabidopsis.Plant and Cell Physiology, 51, 1488-1498

Hirayama T, Shinozaki K. 2010. Research on plant abiotic stressresponses in the post-genome era: Past, present and future.The Plant Journal, 61, 1041-1052

Johansson I, Karlsson M, Johanson U, Larsson C, KjebllbomP. 2000. The role of aquaporins in cellular and whole plant water balance. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1465, 324-342

Jung C, Seo J S, Han S W, Koo Y J, Kim C H, Song S I, Nahm BH, Choi Y D, Cheong J J. 2008. Overexpression of AtMYB44enhances stomatal closure to confer abiotic stress tolerancein transgenic Arabidopsis. Plant Physiology, 146, 623-635

Kansup J, Tsugama D, Liu S, Takano T. 2014. ArabidopsisG-protein β subunit AGB1 interacts with NPH3 and isinvolved in phototropism. Biochemical and BiophysicalResearch Communications, 445, 54-57

Klopffleisch K, Phan N, Augustin K, Bayne R S, Booker K S,Botella J R, Carpita N C, Car T, Chen J G, Cooke T R,Frick-Cheng A, Friedman E J, Fulk B, Hahn M G, Jiang K,Jorda L, Kruppe L, Liu C, Lorek J, McCan M C, et al. 2011.Arabidopsis G-protein interactome reveals connectionsto cell wall carbohydrates and morphogenesis. MolecularSystems Biology, 7, 532.

Lease K A, Wen J, Li J, Doke J T, Liscum E, Walker J C. 2001.A mutant Arabidopsis heterotrimeric G-protein β subunitaffects leaf, flower, and fruit development. The Plant Cell,13, 2631-2641

Livark K J, Schmittgen T D. 2001. Analysis of relative geneexpression data using real-time quantitative PCR and the2−ΔΔCT method. Methods, 25, 402-408

Ma H, Yanofsky M F, Meyerowitz E M. 1990. Molecular cloningand characterization of GPA1, a G protein alpha subunitgene from Arabidopsis thaliana. Proceedings of the NationalAcademy of Sciences of the United States of America, 87,3821-3825

Mansour M M F, Salama K H. 2004. Cellular basis of salinitytolerance in plants. Environmental and ExperimentalBotany, 52, 113-122

Mason M G, Botella J R. 2000. Completing the heterotrimer:isolation and characterization of an Arabidopsis thalianaG protein γ-subunit cDNA. Proceedings of the NationalAcademy of Sciences of the United States of America, 97,14784-14788

Mason M G, Botella J R. 2001. Isolation of a novel G-proteinγ-subunit from Arabidopsis thaliana and its interaction withGβ. Biochimica et Biophysica Acta (BBA)-Gene Structureand Expression, 1520, 147-153

Misra S, Wu Y, Venkataraman G, Sopory S K, Tuteja N. 2007.Heterotrimeric G-protein complex and G-protein-coupledreceptor from a legume (Pisum sativum): Role in salinityand heat stress and cross-talk with phospholipase C. ThePlant Journal, 51, 656-669

Mittler R. 2002. Oxidative stress, antioxidants and stresstolerance. Trends in Plant Science, 7, 405-410

Mudgil Y, Uhrig J F, Zhou J, Temple B, Jiang K, Jones A M.2009. Arabidopsis N-MYC DOWNREGULATED-LIKE1, apositive regulator of auxin transport in a G protein-mediatedpathway. The Plant Cell Online, 21, 3591-3609

Munns R. 2002. Comparative physiology of salt and waterstress. Plant, Cell & Environment, 25, 239-250

Munns R, Tester M. 2008. Mechanisms of salinity tolerance.Annual Review of Plant Biology, 59, 651-681

Murguia J R, Belles J M, Serrano R. 1995. A salt-sensitive 3’(2’),5’-bisphosphate nucleotidase involved in sulfate activation.Science, 267, 232-234

Nilson S E, Assmann S M. 2010. Heterotrimeric G proteinsregulate reproductive trait plasticity in response to wateravailability. New Phytologist, 185, 734-746

Ono K, Yamamoto Y, Hachiya A, Matsumoto H. 1995.Synergistic inhibition of growth by aluminum and iron oftobacco (Nicotiana tabacum L.) cells in suspension culture.Plant and Cell Physiology, 36, 115-125

Oraby H, Ahmad R. 2012. Physiological and biochemicalchanges of CBF3 transgenic oat in response to salinitystress. Plant Science, 185, 331-339

Pandey S, Assmann S M. 2004. The Arabidopsis putative Gprotein-coupled receptor GCR1 interacts with the G proteinα subunit GPA1 and regulates abscisic acid signaling. ThePlant Cell, 16, 1616-1632

Pandey S, Chen J G, Jones A M, Assmann S M. 2006. G-proteincomplex mutants are hypersensitive to abscisic acidregulation of germination and postgermination development.Plant Physiology, 141, 243-256

Persak H, Pitzschke A. 2013. Tight interconnection and multilevelcontrol of Arabidopsis MYB44 in MAPK cascadesignalling. PLOS ONE, 8, e57547.

Qin Y, Wang M, Tian Y, He W, Han L, Xia G. 2012. Overexpressionof TaMYB33 encoding a novel wheat MYBtranscription factor increases salt and drought tolerance inArabidopsis. Molecular Biology Reports, 39, 7183-7192

Roxas V P, Lodhi S A, Garrett D K, Mahan J R, Allen R D.2000. Stress tolerance in transgenic tobacco seedlingsthat overexpress glutathione S-transferase/glutathioneperoxidase. Plant and Cell Physiology, 41, 1229-1234

Shi H T, Li R J, Cai W, Liu W, Wang C L, Lu Y T. 2012.Increasing nitric oxide content in Arabidopsis thaliana byexpressing rat neuronal nitric oxide synthase resulted inenhanced stress tolerance. Plant and Cell Physiology, 53,344-357

Shi H, Ye T, Wang Y, Chan Z. 2013. Arabidopsis ALTEREDMERISTEM PROGRAM 1 negatively modulates plantresponses to abscisic acid and dehydration stress. PlantPhysiology and Biochemistry, 67, 209-216

Tabur S, Demir K. 2010. Role of some growth regulators oncytogenetic activity of barley under salt stress. Plant GrowthRegulation, 60, 99-104

Temple B R, Jones A M. 2007. The plant heterotrimericG-protein complex. Annual Review of Plant Biology, 58,249-266

Tester M, Davenport R. 2003. Na+ tolerance and Na+ transportin higher plants. Annals of Botany, 91, 503-527

Trusov Y, Rookes J E, Chakravorty D, Armour D, Schenk PM, Botella J R. 2006. Heterotrimeric G proteins facilitateArabidopsis resistance to necrotrophic pathogens and areinvolved in jasmonate signaling. Plant Physiology, 140,210-220

Tsugama D, Liu S, Takano T. 2013. A bZIP protein, VIP1,interacts with Arabidopsis heterotrimeric G protein β subunit, AGB1. Plant Physiology and Biochemistry, 71, 240-246

Ullah H, Chen J G, Temple B, Boyes D C, Alonso J M, DavisK R, Ecker J R, Jones A M. 2003. The β-subunit of theArabidopsis G protein negatively regulates auxin-inducedcell division and affects multiple developmental processes.The Plant Cell, 15, 393-409

Ullah H, Chen J G, Wang S, Jones A M. 2002. Role of aheterotrimeric G protein in regulation of Arabidopsis seedgermination. Plant Physiology, 129, 897-907

Uozumi N, Kim E J, Rubio F, Yamaguchi T, Muto S, TsuboiA, Bakker E P, Nakamura T, Schroeder J I. 2000. TheArabidopsis HKT1 gene homolog mediates inward Na+currents in Xenopus laevis oocytes and Na+ uptakein Saccharomyces cerevisiae. Plant Physiology, 122,1249-1260

Wang H X, Weerasinghe R R, Perdue T D, Cakmakci N G,Taylor J P, Marzluff W F, Jones A M. 2006. A Golgi-localizedhexose transporter is involved in heterotrimeric G proteinmediatedearly development in Arabidopsis. MolecularBiology of the Cell, 17, 4257-4269

Wang S, Narendra S, Fedoroff N. 2007. Heterotrimeric G proteinsignaling in the Arabidopsis unfolded protein response.Proceedings of the National Academy of Sciences of theUnited States of America, 104, 3817-3822

Warpeha K M, Lateef S S, Lapik Y, Anderson M, Lee B S,Kaufman L S. 2006. G-protein-coupled receptor 1, G-proteinGα-subunit 1, and prephenate dehydratase 1 are requiredfor blue light-induced production of phenylalanine inetiolated Arabidopsis. Plant Physiology, 140, 844-855

Weiss C A, Garnaat C W, Mukai K, Hu Y, Ma H. 1994. Isolationof cDNAs encoding guanine nucleotide-binding proteinβ-subunit homologues from maize (ZGB1) and Arabidopsis(AGB1). Proceedings of the National Academy of Sciencesof the United States of America, 91, 9554-9558

Xiong L, Lee H, Ishitani M, Zhu J K. 2002. Regulation of osmoticstress-responsive gene expression by the LOS6/ABA1locus in Arabidopsis. Journal of Biological Chemistry, 277,8588-8596

Xu Z S, Xia L Q, Chen M, Cheng X G, Zhang R Y, Li L C, ZhaoY X, Lu Y, Ni Z Y, Liu L. 2007. Isolation and molecularcharacterization of the Triticum aestivum L. ethyleneresponsivefactor 1 (TaERF1) that increases multiple stresstolerance. Plant Molecular Biology, 65, 719-732

Yadav D K, Islam S S, Tuteja N. 2012. Rice heterotrimericG-protein gamma subunits (RGG1 and RGG2) aredifferentially regulated under abiotic stress. Plant Signalingand Behavior, 7, 733-740

Yamaguchi-Shinozaki K, Shinozaki K. 1994. A novel cisactingelement in an Arabidopsis gene is involved inresponsiveness to drought, low-temperature, or high-saltstress. The Plant Cell, 6, 251-264

Zhu J K. 2001. Plant salt tolerance. Trends in Plant Science,6, 66–71.

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G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis

G-protein β subunit AGB1 positively regulates salt stress tolerance in Arabidopsis

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