Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (11): 1732-1738.DOI: 10.1016/S1671-2927(11)60173-9

• 论文 • 上一篇    下一篇

TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance

 ZHANG Li-rong, YANG Wen-xiang , LIU Da-qun   

  1. 1.College of Plant Protection, Biological Control Center of Plant Diseases and Pests of Hebei Province, Agricultural University of Hebei
  • 收稿日期:2010-12-30 出版日期:2011-11-01 发布日期:2011-11-07
  • 通讯作者: Correspondence LIU Da-qun, Professor, Tel: +86-312-7528500, E-mail: ldq@hebau.edu.cn; YANG Wen-xiang, Professor, Tel: +86-312-7528585, E-mail: wenxiangyang2003@163.com
  • 作者简介:ZHANG Li-rong, Tel: +86-312-7528500, E-mail: ZLR592@yahoo.com.cn
  • 基金资助:

    This work was financially supported by the National Natural Science Foundation of China (30771391).

TaRAR1 is Required for Lr24-Mediated Wheat Leaf Rust Resistance

 ZHANG Li-rong, YANG Wen-xiang , LIU Da-qun   

  1. 1.College of Plant Protection, Biological Control Center of Plant Diseases and Pests of Hebei Province, Agricultural University of Hebei
  • Received:2010-12-30 Online:2011-11-01 Published:2011-11-07
  • Contact: Correspondence LIU Da-qun, Professor, Tel: +86-312-7528500, E-mail: ldq@hebau.edu.cn; YANG Wen-xiang, Professor, Tel: +86-312-7528585, E-mail: wenxiangyang2003@163.com
  • About author:ZHANG Li-rong, Tel: +86-312-7528500, E-mail: ZLR592@yahoo.com.cn
  • Supported by:

    This work was financially supported by the National Natural Science Foundation of China (30771391).

摘要: Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RTPCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.

关键词: disease resistance, barley stripe mosaic virus (BSMV), virus-induced gene silencing (VIGS), Lr24, RAR1

Abstract: Virus-induced gene silencing (VIGS) offers a rapid and high throughout technology platform for the analysis of gene function in plants. The barley stripe mosaic virus (BSMV) VIGS system was optimized in studies silencing phytoene desaturase expression in wheat, and demonstrated that infection with BSMV construct carrying a 412 bp fragment of TaRAR1 caused conversion of incompatible to compatible interactions to Lr24-mediated resistance in wheat TcLr24 and cultivar 5R615 harboring Lr24 whereas infection with a control construct had no effect on resistance or susceptibility. RTPCR analysis showed that BSMV-induced gene silencing could be detected at mRNA levels. These studies indicated that TaRAR1 was a required component for Lr24-mediated race-specific resistance and the BSMV-VIGS was a powerful tool for dissecting the genetic pathways of disease resistance in hexaploid wheat.

Key words: disease resistance, barley stripe mosaic virus (BSMV), virus-induced gene silencing (VIGS), Lr24, RAR1