Journal of Integrative Agriculture ›› 2013, Vol. 12 ›› Issue (1): 136-146.DOI: 10.1016/S2095-3119(13)60214-2

• 论文 • 上一篇    下一篇

Identification of Novel and Differentially ExpressedMicroRNAs in the Ovaries of Laying and Non-Laying Ducks

 YU De-bing, JIANG Bao-chun, GONG Jing, DONG Fu-lu, LU Ying-lin, YUE Hui-jie, WANG Zhengchao   

  1. 1.Department of Animal Science and Technology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2.Bioinformatics & Molecular Imaging Key Laboratory, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P.R.China
    3.Provincial Key Laboratory for Developmental Biology and Neurobiology, College of Life Science, Fujian Normal University, Fuzhou 350108, P.R.China
  • 收稿日期:2011-09-30 出版日期:2013-01-01 发布日期:2013-01-24
  • 通讯作者: Correspondence DU Wen-xing, Tel: +86-25-84395106, Fax: +86-25-84395314, E-mail: duwenxing@njau.edu.cn; GUOAn-yuan, E-mail: guoay@mail.hust.edu.cn
  • 作者简介:YU De-bing, E-mail: yudebing@njau.edu.cn;
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (31101705), the Natural Science Foundation of Jiangsu Province, China (BK2010454) and the Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province, China (cx(09)116 and cx (11)1028).

Identification of Novel and Differentially ExpressedMicroRNAs in the Ovaries of Laying and Non-Laying Ducks

 YU De-bing, JIANG Bao-chun, GONG Jing, DONG Fu-lu, LU Ying-lin, YUE Hui-jie, WANG Zhengchao   

  1. 1.Department of Animal Science and Technology, College of Animal Science and Technology, Nanjing Agricultural University, Nanjing 210095, P.R.China
    2.Bioinformatics & Molecular Imaging Key Laboratory, Department of Systems Biology, College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, P.R.China
    3.Provincial Key Laboratory for Developmental Biology and Neurobiology, College of Life Science, Fujian Normal University, Fuzhou 350108, P.R.China
  • Received:2011-09-30 Online:2013-01-01 Published:2013-01-24
  • Contact: Correspondence DU Wen-xing, Tel: +86-25-84395106, Fax: +86-25-84395314, E-mail: duwenxing@njau.edu.cn; GUOAn-yuan, E-mail: guoay@mail.hust.edu.cn
  • About author:YU De-bing, E-mail: yudebing@njau.edu.cn;
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (31101705), the Natural Science Foundation of Jiangsu Province, China (BK2010454) and the Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province, China (cx(09)116 and cx (11)1028).

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摘要： MicroRNAs (miRNAs), which post-transcriptionally regulate gene expression by binding to the 3´ untranslated region of mRNAs to either inhibit or enhance translation, are involved in diverse biological processes. The use of high-throughput Solexa sequencing plays important roles in the discovery of miRNAs. In this study, we used high-throughput Solexa sequencing to identify novel duck miRNAs and compare the miRNA expression profiles in laying and non-laying duck ovaries. Using a bioinformatic analysis, we discovered 86 potential duck miRNAs similar to known chicken miRNAs and 43 unique sequences that matched known miRNAs of other species. We also found that miRNA variations and isoforms were widespread in our two RNA libraries, with most of the variations occurring in the 3´ region of the miRNAs. Furthermore, we detected 55 miRNAs that exhibited significant expression differences between laying and non-laying ducks. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses of the potential targets of the differentially expressed miRNAs indicated these miRNAs may play key roles in the egg laying process. Finally, we confirmed the differential expression of 5 miRNAs in the laying and non-laying samples by qRT-PCR. Cumulatively, our work provides the first look at the miRNA expression profile of the duck ovary and provides novel insight into the roles of miRNAs in egg laying and reproduction.

关键词: microRNA , Solexa sequencing , duck , ovary

Abstract: MicroRNAs (miRNAs), which post-transcriptionally regulate gene expression by binding to the 3´ untranslated region of mRNAs to either inhibit or enhance translation, are involved in diverse biological processes. The use of high-throughput Solexa sequencing plays important roles in the discovery of miRNAs. In this study, we used high-throughput Solexa sequencing to identify novel duck miRNAs and compare the miRNA expression profiles in laying and non-laying duck ovaries. Using a bioinformatic analysis, we discovered 86 potential duck miRNAs similar to known chicken miRNAs and 43 unique sequences that matched known miRNAs of other species. We also found that miRNA variations and isoforms were widespread in our two RNA libraries, with most of the variations occurring in the 3´ region of the miRNAs. Furthermore, we detected 55 miRNAs that exhibited significant expression differences between laying and non-laying ducks. Gene ontology (GO) and kyoto encyclopedia of genes and genomes (KEGG) enrichment analyses of the potential targets of the differentially expressed miRNAs indicated these miRNAs may play key roles in the egg laying process. Finally, we confirmed the differential expression of 5 miRNAs in the laying and non-laying samples by qRT-PCR. Cumulatively, our work provides the first look at the miRNA expression profile of the duck ovary and provides novel insight into the roles of miRNAs in egg laying and reproduction.

Key words: microRNA , Solexa sequencing , duck , ovary