Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)

doi:10.1016/S1671-2927(11)60183-1

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (12): 1834-1841.DOI: 10.1016/S1671-2927(11)60183-1

Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)

JIANG Wei, YANG Shou-ping, YU De-yue and GAI Jun-yi

1.Soybean Research Institute, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.China

收稿日期:2011-01-28 出版日期:2011-12-01 发布日期:2011-12-19
通讯作者: Correspondence YANG Shou-ping, Tel: +86-25-84396463, E-mail: spyung@126.com; GAI Jun-yi, Tel: +86-25-84395405,E-mail: sri@njau.edu.cn
作者简介:JIANG Wei, E-mail: jiangww109@163.com
基金资助:
The project was supported by the National High-Tech R&D Program of China (2009AA101106, 2011AA10A105) and the National Key Program for Transgenic Breeding of China (2008ZX08004-005, 2011ZX08004, 2011ZX08-005).

Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)

JIANG Wei, YANG Shou-ping, YU De-yue and GAI Jun-yi

1.Soybean Research Institute, Nanjing Agricultural University/National Center for Soybean Improvement/National Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing 210095, P.R.China

Received:2011-01-28 Online:2011-12-01 Published:2011-12-19
Contact: Correspondence YANG Shou-ping, Tel: +86-25-84396463, E-mail: spyung@126.com; GAI Jun-yi, Tel: +86-25-84395405,E-mail: sri@njau.edu.cn
About author:JIANG Wei, E-mail: jiangww109@163.com
Supported by:
The project was supported by the National High-Tech R&D Program of China (2009AA101106, 2011AA10A105) and the National Key Program for Transgenic Breeding of China (2008ZX08004-005, 2011ZX08004, 2011ZX08-005).

摘要/Abstract

摘要： Cytoplasmic male sterility plays an important role in utilization of crop heterosis. Screening of soybean for novel genes related to male sterility in soybean could provide a basis for studying the molecular mechanism of male sterility in plants. In this study, gene differential expressions between the cytoplasmic male-sterile line NJCMS1A and its maintainer line NJCMS1B in soybean were analyzed using cDNA-AFLP. A differentially expressed fragment, GmMF-T4A15, was isolated from large flower buds of NJCMS1B. By searching the soybean genomic library and PCR amplification, the cDNA fulllength sequence of 1 311 bp was obtained and named GmMF1. The expression characteristics of GmMF1 were studied by semiquantitative real-time PCR and real-time quantitative PCR. The results showed that GmMF1 was expressed highly in flower buds of NJCMS1B. The deduced protein contains 436 amino acids and shows high similarity to members of the DUF620 protein family with unknown functions in other plant species. It is predicted that the protein encoded by GmMF1 is localized in the nucleus.

关键词: soybean, cytoplasmic male sterility, cDNA-AFLP, gene differential expressions

Abstract: Cytoplasmic male sterility plays an important role in utilization of crop heterosis. Screening of soybean for novel genes related to male sterility in soybean could provide a basis for studying the molecular mechanism of male sterility in plants. In this study, gene differential expressions between the cytoplasmic male-sterile line NJCMS1A and its maintainer line NJCMS1B in soybean were analyzed using cDNA-AFLP. A differentially expressed fragment, GmMF-T4A15, was isolated from large flower buds of NJCMS1B. By searching the soybean genomic library and PCR amplification, the cDNA fulllength sequence of 1 311 bp was obtained and named GmMF1. The expression characteristics of GmMF1 were studied by semiquantitative real-time PCR and real-time quantitative PCR. The results showed that GmMF1 was expressed highly in flower buds of NJCMS1B. The deduced protein contains 436 amino acids and shows high similarity to members of the DUF620 protein family with unknown functions in other plant species. It is predicted that the protein encoded by GmMF1 is localized in the nucleus.

Key words: soybean, cytoplasmic male sterility, cDNA-AFLP, gene differential expressions

JIANG Wei, YANG Shou-ping, YU De-yue and GAI Jun-yi. Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)[J]. Journal of Integrative Agriculture, 2011, 10(12): 1834-1841.

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Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)

Cloning and Characterization of a Novel Gene GmMF1 in Soybean (Glycine max L. Merr.)

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