Relationship Between Differential Expression of Bax and Bcl-2 Genes and Developmental Differences of Porcine Parthenotes Cultured in PZM-3 and NCSU-23

doi:10.1016/S1671-2927(11)60177-6

Journal of Integrative Agriculture ›› 2011, Vol. 10 ›› Issue (11): 1772-1780.DOI: 10.1016/S1671-2927(11)60177-6

Relationship Between Differential Expression of Bax and Bcl-2 Genes and Developmental Differences of Porcine Parthenotes Cultured in PZM-3 and NCSU-23

CUI Mao-sheng, LIU Zhen-xing, WANG Xian-long, ZHANG Jing, WU Yi, HAN Guo-cai , ZENG Shen-ming

1.Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
2.Tianjin Institute of Animal Science

收稿日期:2010-10-08 出版日期:2011-11-01 发布日期:2011-11-07
通讯作者: Correspondence ZENG Shen-ming, Professor, Ph D, Tel/Fax: +86-10-62733744, E-mail: zengsm@cau.edu.cn; HAN Guo-cai, Associate Professor, Tel/Fax: +86-10-62733388, E-mail: hanguocai@263.net
作者简介:CUI Mao-sheng, Ph D, E-mail: cuimaosheng@gmail.com; LIU Zhen-xing, Ph D, E-mail: sgliuzhenxing@163.com
基金资助:
This research was funded by the National Natural Science Foundation of China (31072031) and the Key Research Program of Ministry of Education of China (109018).

Relationship Between Differential Expression of Bax and Bcl-2 Genes and Developmental Differences of Porcine Parthenotes Cultured in PZM-3 and NCSU-23

CUI Mao-sheng, LIU Zhen-xing, WANG Xian-long, ZHANG Jing, WU Yi, HAN Guo-cai , ZENG Shen-ming

1.Laboratory of Animal Embryonic Biotechnology, College of Animal Science and Technology, China Agricultural University
2.Tianjin Institute of Animal Science

Received:2010-10-08 Online:2011-11-01 Published:2011-11-07
Contact: Correspondence ZENG Shen-ming, Professor, Ph D, Tel/Fax: +86-10-62733744, E-mail: zengsm@cau.edu.cn; HAN Guo-cai, Associate Professor, Tel/Fax: +86-10-62733388, E-mail: hanguocai@263.net
About author:CUI Mao-sheng, Ph D, E-mail: cuimaosheng@gmail.com; LIU Zhen-xing, Ph D, E-mail: sgliuzhenxing@163.com
Supported by:
This research was funded by the National Natural Science Foundation of China (31072031) and the Key Research Program of Ministry of Education of China (109018).

摘要/Abstract

摘要： The developmental competence of porcine parthenotes cultured in porcine zygote medium-3 (PZM-3) and North Carolina State University-23 (NCSU-23) media was investigated. After in vitro maturation oocytes were electro-activated, and the subsequent developmental competence, rates of apoptotic, fragmented and arrested embryos from the two media were evaluated. Also, the ratio of mRNA expression of Bcl-2 and Bax gene was determined. Results demonstrated that cleavage, blastocyst, hatched blastocyst rates, and blastocyst cell numbers were significantly higher in PZM-3 than in NCSU-23. The rate of fragmented embryos in PZM-3 was lower than in NCSU-23 on d 1 and 3 (P<0.05) while the percentage of arrested embryo was lower in PZM-3 than in NCSU-23 on d 4 and 5 (P<0.05). TUNEL positive signals were higher in NCSU-23 than in PZM-3 from d 3 to 7 (P<0.05). The ratios of Bcl-2 and Bax mRNA expression in embryos were higher on d 5 than on d 3 and 1 in PZM-3 (P<0.05). In contrast, the ratios of Bcl-2 and Bax mRNA expression in embryos on d 1 were higher than on d 3 and 5 in NCSU-23 (P<0.05). Additionally, the ratios of Bcl-2 and Bax mRNA expression in embryos in PZM-3 were higher than in NCSU-23 on d 3 and 5 (P<0.05). In conclusion, lower apoptotic embryo rates and down-regulating Bax together with up-regulating expression of Bcl-2 transcripts may be responsible for the better developmental competence of porcine parthenotes in PZM-3.

关键词: apoptosis, parthenote, developmental potential, culture medium, porcine

Abstract: The developmental competence of porcine parthenotes cultured in porcine zygote medium-3 (PZM-3) and North Carolina State University-23 (NCSU-23) media was investigated. After in vitro maturation oocytes were electro-activated, and the subsequent developmental competence, rates of apoptotic, fragmented and arrested embryos from the two media were evaluated. Also, the ratio of mRNA expression of Bcl-2 and Bax gene was determined. Results demonstrated that cleavage, blastocyst, hatched blastocyst rates, and blastocyst cell numbers were significantly higher in PZM-3 than in NCSU-23. The rate of fragmented embryos in PZM-3 was lower than in NCSU-23 on d 1 and 3 (P<0.05) while the percentage of arrested embryo was lower in PZM-3 than in NCSU-23 on d 4 and 5 (P<0.05). TUNEL positive signals were higher in NCSU-23 than in PZM-3 from d 3 to 7 (P<0.05). The ratios of Bcl-2 and Bax mRNA expression in embryos were higher on d 5 than on d 3 and 1 in PZM-3 (P<0.05). In contrast, the ratios of Bcl-2 and Bax mRNA expression in embryos on d 1 were higher than on d 3 and 5 in NCSU-23 (P<0.05). Additionally, the ratios of Bcl-2 and Bax mRNA expression in embryos in PZM-3 were higher than in NCSU-23 on d 3 and 5 (P<0.05). In conclusion, lower apoptotic embryo rates and down-regulating Bax together with up-regulating expression of Bcl-2 transcripts may be responsible for the better developmental competence of porcine parthenotes in PZM-3.

Key words: apoptosis, parthenote, developmental potential, culture medium, porcine

CUI Mao-sheng, LIU Zhen-xing, WANG Xian-long, ZHANG Jing, WU Yi, HAN Guo-cai , ZENG Shen-ming . Relationship Between Differential Expression of Bax and Bcl-2 Genes and Developmental Differences of Porcine Parthenotes Cultured in PZM-3 and NCSU-23 [J]. Journal of Integrative Agriculture, 2011, 10(11): 1772-1780.

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Relationship Between Differential Expression of Bax and Bcl-2 Genes and Developmental Differences of Porcine Parthenotes Cultured in PZM-3 and NCSU-23

Relationship Between Differential Expression of Bax and Bcl-2 Genes and Developmental Differences of Porcine Parthenotes Cultured in PZM-3 and NCSU-23

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