Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (12): 2058-2066.DOI: 10.1016/S1671-2927(00)8744

• 论文 • 上一篇    下一篇

Induction ofApoptosis in the ImmatureMouse Testes by aMixture ofMelamine and CyanuricAcid

 YOU Hua, ZHU Jin-feng, SHE Rui-ping, CHANG Ling-ling, SHI Rui-han, DING Ye, CHI Li-juan, LIUBin, YUE Zhuo, TIAN Ji-jing, MAO Jing-jing , SU Li-fang   

  1. 1.Key Laboratory of Zoonosis, Ministry of Agriculture/Department of Veterinary Pathology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China
    2.China Animal Disease Control Center, Beijing 100125, P.R.China
    3.National Animal Husbandry Service, Beijing 100125, P.R.China
  • 收稿日期:2012-04-20 出版日期:2012-12-01 发布日期:2012-12-18
  • 通讯作者: Correspondence SHE Rui-ping, Tel: +86-10-62733060, Fax: +86-10-62733321, E-mail: sheruiping@126.com
  • 作者简介:YOU Hua, E-mail: hyyouhua@yahoo.com.cn
  • 基金资助:

    This work was supported by the National Natural Science Foundation of China (30871853). We declare that the experiments complied with the current laws of China where they were performed.

Induction ofApoptosis in the ImmatureMouse Testes by aMixture ofMelamine and CyanuricAcid

 YOU Hua, ZHU Jin-feng, SHE Rui-ping, CHANG Ling-ling, SHI Rui-han, DING Ye, CHI Li-juan, LIUBin, YUE Zhuo, TIAN Ji-jing, MAO Jing-jing , SU Li-fang   

  1. 1.Key Laboratory of Zoonosis, Ministry of Agriculture/Department of Veterinary Pathology, College of Veterinary Medicine, China Agricultural University, Beijing 100193, P.R.China
    2.China Animal Disease Control Center, Beijing 100125, P.R.China
    3.National Animal Husbandry Service, Beijing 100125, P.R.China
  • Received:2012-04-20 Online:2012-12-01 Published:2012-12-18
  • Contact: Correspondence SHE Rui-ping, Tel: +86-10-62733060, Fax: +86-10-62733321, E-mail: sheruiping@126.com
  • About author:YOU Hua, E-mail: hyyouhua@yahoo.com.cn
  • Supported by:

    This work was supported by the National Natural Science Foundation of China (30871853). We declare that the experiments complied with the current laws of China where they were performed.

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摘要： The toxicity of melamine has attracted much attention since the recent outbreaks of renal injury in pets and infants. Previous studies indicated that melamine by itself had low toxicity, whereas a mixture of melamine and cyanuric acid (M+CA) could cause serious renal damage. At present, most researches on the toxicity of M+CA are focused on the kidney. However, little is known about the adverse effects of this mixture on the reproductive system. In the present study, the toxicity of M+CA to testes was investigated. Immature male mice were orally dosed with 0, 0.6, 3, and 15 mg kg-1 d-1 of a 1:1 M+CA for 28 d. Pathological changes occurred in germ cells, such as loose arrangement, reduced numbers and karyopyknosis, indicating that this mixture was toxic to spermatogenesis. Compared with the control group, the TUNELpositive germ cells increased significantly and the ratio of Bcl-2 to Bax, total antioxidant capacity and superoxide dismutase activity decreased significantly in the 3 and 15 mg kg-1 d-1 M+CA treated group, while the activities of caspase-3, caspase- 8 and caspase-9 remained unchanged. The results suggest that M+CA can induce apoptosis in the mice testes. The downregulation of Bcl-2/Bax and oxidative stress may play a pivotal role in the induction of apoptosis by M+CA in mice testes.

关键词: melamine/cyanuric acid mixture, testes, injury, caspase-independent apoptosis

Abstract: The toxicity of melamine has attracted much attention since the recent outbreaks of renal injury in pets and infants. Previous studies indicated that melamine by itself had low toxicity, whereas a mixture of melamine and cyanuric acid (M+CA) could cause serious renal damage. At present, most researches on the toxicity of M+CA are focused on the kidney. However, little is known about the adverse effects of this mixture on the reproductive system. In the present study, the toxicity of M+CA to testes was investigated. Immature male mice were orally dosed with 0, 0.6, 3, and 15 mg kg-1 d-1 of a 1:1 M+CA for 28 d. Pathological changes occurred in germ cells, such as loose arrangement, reduced numbers and karyopyknosis, indicating that this mixture was toxic to spermatogenesis. Compared with the control group, the TUNELpositive germ cells increased significantly and the ratio of Bcl-2 to Bax, total antioxidant capacity and superoxide dismutase activity decreased significantly in the 3 and 15 mg kg-1 d-1 M+CA treated group, while the activities of caspase-3, caspase- 8 and caspase-9 remained unchanged. The results suggest that M+CA can induce apoptosis in the mice testes. The downregulation of Bcl-2/Bax and oxidative stress may play a pivotal role in the induction of apoptosis by M+CA in mice testes.

Key words: melamine/cyanuric acid mixture, testes, injury, caspase-independent apoptosis