Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development

doi:10.1016/S2095-3119(13)60419-0

Journal of Integrative Agriculture ›› 2014, Vol. 13 ›› Issue (6): 1331-1339.DOI: 10.1016/S2095-3119(13)60419-0

Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development

YANG Ya-lan, LI Yan, LIANG Ru-yi, ZHOU Rong, AO Hong, MU Yu-lian, YANG Shu-lin, LI Kui , TANG Zhong-lin

1、Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese
Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University,
Nanjing 210095, P.R.China

收稿日期:2013-01-29 出版日期:2014-06-01 发布日期:2014-06-06
通讯作者: TANG Zhong-lin, Tel/Fax: +86-10-62818180, E-mail: zhonglinqy_99@sina.com
作者简介:YANG Ya-lan, E-mail: yangyalan1988@126.com
基金资助:
This study was supported by the National Key Project (2013ZX08009-001), the National Basic Research Program of China (2012CB124706-6) and the Agricul tural Science and Technology Innovation Program (ASTIP-IAS05).

Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development

YANG Ya-lan, LI Yan, LIANG Ru-yi, ZHOU Rong, AO Hong, MU Yu-lian, YANG Shu-lin, LI Kui , TANG Zhong-lin

1、Key Laboratory of Farm Animal Genetic Resources and Germplasm Innovation, Ministry of Agriculture/Institute of Animal Science, Chinese
Academy of Agricultural Sciences, Beijing 100193, P.R.China
2、Department of Animal Genetics, Breeding and Reproduction, College of Animal Science and Technology, Nanjing Agricultural University,
Nanjing 210095, P.R.China

Received:2013-01-29 Online:2014-06-01 Published:2014-06-06
Contact: TANG Zhong-lin, Tel/Fax: +86-10-62818180, E-mail: zhonglinqy_99@sina.com
About author:YANG Ya-lan, E-mail: yangyalan1988@126.com
Supported by:
This study was supported by the National Key Project (2013ZX08009-001), the National Basic Research Program of China (2012CB124706-6) and the Agricul tural Science and Technology Innovation Program (ASTIP-IAS05).

摘要/Abstract

摘要： MicroRNAs (miRNAs), evolutionarily conserved non-coding RNAs in length 21-24 bp, play a critical role in skeletal muscle development. In this study, to explore the function of mircoRNA-127 in porcine skeletal muscle development, eight tissue samples from adult pigs and longissimus muscle samples at 26 developmental stages were collected from Tongcheng and Landrace pigs. The spatial-temporal expression profiles of miRNA-127 were carried out using step-loop quantitative real-time PCR (stem- loop RT-PCR). To explore the molecular functions of miRNA-127, we predicted its target genes and performed functional annotation using bioinformatics methods. Results suggested that miRNA-127 was abundantly expressed in heart, ovary, uterus and spleen tissues and was weakly expressed in liver, lung, kidney and small intestine in both Tongcheng and Landrace pigs. And miRNA-127 showed significant expression differences in heart, ovary, spleen and uterus tissues between these two breeds. miRNA-127 basically kept at a relatively stable high level in middle and later embryonic stages and a low expression level in early embryonic stages and postnatal stages, but the expression levels of miRNA-127 were higher in Tongcheng pigs than in Landrace at most developmental stages. miRNA-127 potentially regulated 240 candidate genes. Results of Gene Ontology and KEGG pathway analysis indicated that these genes could be involved in many molecular functions and mechanisms, such as regulation of the force of heart contraction, regulation of transcription, regulation of T cell differentiation, MAPK signaling pathway and GnRH signaling pathway. Many significantly enriched GO terms and KEGG pathways were related to skeletal muscle development. This study will be helpful to understand the biological function for miRNA-127 and identify candidate gene associated with meat production traits in pigs.

关键词: porcine , microRNA-127 , expression profiles , skeletal muscle development , target prediction

Abstract: MicroRNAs (miRNAs), evolutionarily conserved non-coding RNAs in length 21-24 bp, play a critical role in skeletal muscle development. In this study, to explore the function of mircoRNA-127 in porcine skeletal muscle development, eight tissue samples from adult pigs and longissimus muscle samples at 26 developmental stages were collected from Tongcheng and Landrace pigs. The spatial-temporal expression profiles of miRNA-127 were carried out using step-loop quantitative real-time PCR (stem- loop RT-PCR). To explore the molecular functions of miRNA-127, we predicted its target genes and performed functional annotation using bioinformatics methods. Results suggested that miRNA-127 was abundantly expressed in heart, ovary, uterus and spleen tissues and was weakly expressed in liver, lung, kidney and small intestine in both Tongcheng and Landrace pigs. And miRNA-127 showed significant expression differences in heart, ovary, spleen and uterus tissues between these two breeds. miRNA-127 basically kept at a relatively stable high level in middle and later embryonic stages and a low expression level in early embryonic stages and postnatal stages, but the expression levels of miRNA-127 were higher in Tongcheng pigs than in Landrace at most developmental stages. miRNA-127 potentially regulated 240 candidate genes. Results of Gene Ontology and KEGG pathway analysis indicated that these genes could be involved in many molecular functions and mechanisms, such as regulation of the force of heart contraction, regulation of transcription, regulation of T cell differentiation, MAPK signaling pathway and GnRH signaling pathway. Many significantly enriched GO terms and KEGG pathways were related to skeletal muscle development. This study will be helpful to understand the biological function for miRNA-127 and identify candidate gene associated with meat production traits in pigs.

Key words: porcine , microRNA-127 , expression profiles , skeletal muscle development , target prediction

YANG Ya-lan, LI Yan, LIANG Ru-yi, ZHOU Rong, AO Hong, MU Yu-lian, YANG Shu-lin, LI Kui , TANG Zhong-lin. Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development[J]. Journal of Integrative Agriculture, 2014, 13(6): 1331-1339.

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Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development

Dynamic Expression of MicroRNA-127 During Porcine Prenatal and Postnatal Skeletal Muscle Development

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