Identification of Organic Substances Associated with Tissue Senescence in Upland Cotton (Gossypium spp. L.) Based on GC-MS Analysis

doi:10.1016/S1671-2927(11)60110-7

摘要/Abstract

摘要： Premature senescence in crop production, especially occurred at the late growth stage, generally results in a reduction inyield and quality. Therefore, it is beneficial for yield and quality to properly delay senescence of plant tissues during thelate developmental stage. In this study, it was observed that the chlorophyll content and photosynthetic rate weregradually decreased along leaf growth progression, and the rates of reduction were promoted by drought. Based on gaschromatography-mass spectrometry (GC-MS) analysis, total eight, five, seven, and five kinds of organic compounds thatputatively associated with the tissue senescent progression were identified in leaves, fruit branches, petals, and sepals,respectively. It was found that the identified organic compound, such as α-pinene, β-pinene, and pentadecane werepresent in different tissues. Among the total ten organic substances identified to be related with the leaf senescence, halfwere specifically detected in the drought treatment. These results suggest some biochemical pathways associated withthe leaf senescence are distinctly regulated by drought. The identified organic compounds in the tested tissues showedthree types on the performance pattern based on the contents along with the senescent progression, including graduallyincreasing, decreasing, and a curve with one single peak. Thus, during the senescence process in tissues, a subset ofmetabolic substances occur modifications on the quantities, reflecting a complicate biochemical reactions are initiated viathe senescence signals. Further analysis of the important organic substances will be helpful for elucidation of the tissuesenescence mechanism at the biochemical level and provide a new insight of the senescence signaling transductions incotton.

关键词:

cotton (Gossypium spp. L.), senescence, organic substance, identification

Abstract: Premature senescence in crop production, especially occurred at the late growth stage, generally results in a reduction inyield and quality. Therefore, it is beneficial for yield and quality to properly delay senescence of plant tissues during thelate developmental stage. In this study, it was observed that the chlorophyll content and photosynthetic rate weregradually decreased along leaf growth progression, and the rates of reduction were promoted by drought. Based on gaschromatography-mass spectrometry (GC-MS) analysis, total eight, five, seven, and five kinds of organic compounds thatputatively associated with the tissue senescent progression were identified in leaves, fruit branches, petals, and sepals,respectively. It was found that the identified organic compound, such as α-pinene, β-pinene, and pentadecane werepresent in different tissues. Among the total ten organic substances identified to be related with the leaf senescence, halfwere specifically detected in the drought treatment. These results suggest some biochemical pathways associated withthe leaf senescence are distinctly regulated by drought. The identified organic compounds in the tested tissues showedthree types on the performance pattern based on the contents along with the senescent progression, including graduallyincreasing, decreasing, and a curve with one single peak. Thus, during the senescence process in tissues, a subset ofmetabolic substances occur modifications on the quantities, reflecting a complicate biochemical reactions are initiated viathe senescence signals. Further analysis of the important organic substances will be helpful for elucidation of the tissuesenescence mechanism at the biochemical level and provide a new insight of the senescence signaling transductions incotton.

Key words:

cotton (Gossypium spp. L.), senescence, organic substance, identification

XU Zhen-long, GUO Cheng-jin, GU Jun-tao, LU Wen-jing, LI Xiao-juan, XIAO Kai. Identification of Organic Substances Associated with Tissue Senescence in Upland Cotton (Gossypium spp. L.) Based on GC-MS Analysis[J]. Journal of Integrative Agriculture, 2011, 10(8): 1197-1205.

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