Ultrastructural Changes of Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci in Cucumber After Treated by Chrysophanol

doi:10.1016/S1671-2927(00)8620

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (6): 970-977.DOI: 10.1016/S1671-2927(00)8620

Ultrastructural Changes of Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci in Cucumber After Treated by Chrysophanol

REN Hong-min, FAN Fan, CAO Ke-qiang

Department of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China

收稿日期:2011-05-12 出版日期:2012-06-01 发布日期:2012-07-20
通讯作者: CAO Ke-qiang, Tel: +86-312-7528145, E-mail: cao.keqiang@yahoo.com
作者简介:REN Hong-min, Tel: +86-312-7528157, E-mail: auhrhm@163.com
基金资助:
This project was supported by the Special Fund for Agroscientific Research in the Public Interest, Ministry of Agriculture of China (200903004). We thank Professor Kang Zhensheng, Northwest A&F University, China, for his excellent technical assistance.

Ultrastructural Changes of Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci in Cucumber After Treated by Chrysophanol

REN Hong-min, FAN Fan, CAO Ke-qiang

Department of Plant Protection, Agricultural University of Hebei, Baoding 071001, P.R.China

Received:2011-05-12 Online:2012-06-01 Published:2012-07-20
Contact: CAO Ke-qiang, Tel: +86-312-7528145, E-mail: cao.keqiang@yahoo.com
About author:REN Hong-min, Tel: +86-312-7528157, E-mail: auhrhm@163.com
Supported by:
This project was supported by the Special Fund for Agroscientific Research in the Public Interest, Ministry of Agriculture of China (200903004). We thank Professor Kang Zhensheng, Northwest A&F University, China, for his excellent technical assistance.

摘要/Abstract

摘要： Chrysophanol (1,8-dihydroxy-3-methylanthraquinone) is a free anthraquinone compound and a secondary metabolite of medicinal plant rhubarb. Chrysophanol has been reported to have both protective and curative activity against Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci, the causal agent of cucumber powdery mildew. In this paper the ultrastructure of powdery mildew on cucumber leaves was studied using electron microscopy after the leaves were treated with chrysophanol. Results showed that preventive treatments with chrysophanol affected fungal development, including spore germination, appressorial formation, and penetration. In the curative treatment, chrysophanol affected fungal survival, resulting in broken cell wall of germ tubes, swelling and collapse of hyphal tips, hyphal malformation, delayed and reduced sporulation. The morphological changes induced by chrysophanol at the ultrastructural level were reflected by haustorium deformation, vacuolization, abortion, and necrosis. Host cell walls infected or adjacent to haustoria were thickened. All these morphological changes of S. fuliginea further confirmed the fungicidal activity of chrysophanol on powdery mildew of cucumber.

关键词: chrysophanol, electronic microscopy, powdery mildew, ultrastructure

Abstract: Chrysophanol (1,8-dihydroxy-3-methylanthraquinone) is a free anthraquinone compound and a secondary metabolite of medicinal plant rhubarb. Chrysophanol has been reported to have both protective and curative activity against Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci, the causal agent of cucumber powdery mildew. In this paper the ultrastructure of powdery mildew on cucumber leaves was studied using electron microscopy after the leaves were treated with chrysophanol. Results showed that preventive treatments with chrysophanol affected fungal development, including spore germination, appressorial formation, and penetration. In the curative treatment, chrysophanol affected fungal survival, resulting in broken cell wall of germ tubes, swelling and collapse of hyphal tips, hyphal malformation, delayed and reduced sporulation. The morphological changes induced by chrysophanol at the ultrastructural level were reflected by haustorium deformation, vacuolization, abortion, and necrosis. Host cell walls infected or adjacent to haustoria were thickened. All these morphological changes of S. fuliginea further confirmed the fungicidal activity of chrysophanol on powdery mildew of cucumber.

Key words: chrysophanol, electronic microscopy, powdery mildew, ultrastructure

REN Hong-min, FAN Fan, CAO Ke-qiang. Ultrastructural Changes of Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci in Cucumber After Treated by Chrysophanol[J]. Journal of Integrative Agriculture, 2012, 12(6): 970-977.

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Ultrastructural Changes of Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci in Cucumber After Treated by Chrysophanol

Ultrastructural Changes of Sphaerotheca fuliginea (Schlechtend.:Fr.) Pollacci in Cucumber After Treated by Chrysophanol

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