Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.

doi:10.1016/S1671-2927(00)8619

Journal of Integrative Agriculture ›› 2012, Vol. 12 ›› Issue (6): 962-969.DOI: 10.1016/S1671-2927(00)8619

Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.

HE Wen-ai, HUANG Da-hui, LI Rong-bai, YANG Hai-ning, HUANG Yue-yue, LIU Chi, MA Zeng-feng, YANG Yong

1.Guangxi University Library, Guangxi University, Nanning 530004, P.R.China
2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Rice Research Institute, Guangxi Academy of Agricultural Sciences,Nanning 530007, P.R.China
3.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, P.R.China
4.Agricultural College, Guangxi University, Nanning 530004, P.R.China

收稿日期:2011-09-22 出版日期:2012-06-01 发布日期:2012-07-20
通讯作者: LI Rong-bai, Mobile: 13517664886, E-mail: lirongbai@126.com; HUANG Da-hui, Mobile: 13878852855, E-mail: hdh1103@163.com
作者简介:HE Wen-ai, Tel/Fax: +86-771-3271163, Mobile: 13978685143, E-mail: hwamch@163.com
基金资助:
This research was supported by the Science Foundation of Guangxi University, China (XDZ110082), the National Natural Science Foundation of China (31000703), the Guangxi Science and Technology Projects, China (1123001- 3B), the Guangxi Science Foundation of China (0833078) and the Fundamental Research Funds for Guangxi Academy of Agricultural Sciences, China (200801Z and 200918J).

Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.

HE Wen-ai, HUANG Da-hui, LI Rong-bai, YANG Hai-ning, HUANG Yue-yue, LIU Chi, MA Zeng-feng, YANG Yong

1.Guangxi University Library, Guangxi University, Nanning 530004, P.R.China
2.Guangxi Crop Genetic Improvement and Biotechnology Laboratory/Rice Research Institute, Guangxi Academy of Agricultural Sciences,Nanning 530007, P.R.China
3.State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, Nanning 530004, P.R.China
4.Agricultural College, Guangxi University, Nanning 530004, P.R.China

Received:2011-09-22 Online:2012-06-01 Published:2012-07-20
Contact: LI Rong-bai, Mobile: 13517664886, E-mail: lirongbai@126.com; HUANG Da-hui, Mobile: 13878852855, E-mail: hdh1103@163.com
About author:HE Wen-ai, Tel/Fax: +86-771-3271163, Mobile: 13978685143, E-mail: hwamch@163.com
Supported by:
This research was supported by the Science Foundation of Guangxi University, China (XDZ110082), the National Natural Science Foundation of China (31000703), the Guangxi Science and Technology Projects, China (1123001- 3B), the Guangxi Science Foundation of China (0833078) and the Fundamental Research Funds for Guangxi Academy of Agricultural Sciences, China (200801Z and 200918J).

摘要/Abstract

摘要： Bacterial leaf streak (BLS) of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a worldwide destructive disease. Development of resistant varieties is considered to be one of the most effective and eco-friendly ways to control the disease. However, only a few genes/QTLs having resistance to BLS have been identified in rice until now. In the present study, we have identified and primarily mapped a BLS-resistance gene, bls1, from a rice line DP3, derived from the wild rice species Oryza rufipogon Griff. A BC2F2 (9311/DP3//9311) population was constructed to map BLS-resistance gene in the rice line DP3. The segregation of the resistant and susceptible plants in BC2F2 in 1:3 ratio (χ2=0.009, χ2 0.05, 1=3.84, P>0.05), suggested that a recessive gene confers BLS resistance in DP3. In bulked segregant analysis (BSA), two SSR markers RM8116 and RM584 were identified to be polymorphic in resistant and susceptible DNA bulks. For further mapping the resistance gene, six polymorphic markers around the target region were applied to analyze the genotypes of the BC2F2 individuals. As a result, the BLS-resistant gene, designated as bls1, was mapped in a 4.0-cM region flanked by RM587 and RM510 on chromosome 6.

关键词: bacterial leaf streak, Xanthomonas oryzae pv. oryzicola, Oryza rufipogon Griff., gene mapping, markerassisted selection (MAS)

Abstract: Bacterial leaf streak (BLS) of rice, caused by Xanthomonas oryzae pv. oryzicola (Xoc) is a worldwide destructive disease. Development of resistant varieties is considered to be one of the most effective and eco-friendly ways to control the disease. However, only a few genes/QTLs having resistance to BLS have been identified in rice until now. In the present study, we have identified and primarily mapped a BLS-resistance gene, bls1, from a rice line DP3, derived from the wild rice species Oryza rufipogon Griff. A BC2F2 (9311/DP3//9311) population was constructed to map BLS-resistance gene in the rice line DP3. The segregation of the resistant and susceptible plants in BC2F2 in 1:3 ratio (χ2=0.009, χ2 0.05, 1=3.84, P>0.05), suggested that a recessive gene confers BLS resistance in DP3. In bulked segregant analysis (BSA), two SSR markers RM8116 and RM584 were identified to be polymorphic in resistant and susceptible DNA bulks. For further mapping the resistance gene, six polymorphic markers around the target region were applied to analyze the genotypes of the BC2F2 individuals. As a result, the BLS-resistant gene, designated as bls1, was mapped in a 4.0-cM region flanked by RM587 and RM510 on chromosome 6.

Key words: bacterial leaf streak, Xanthomonas oryzae pv. oryzicola, Oryza rufipogon Griff., gene mapping, markerassisted selection (MAS)

HE Wen-ai, HUANG Da-hui, LI Rong-bai, YANG Hai-ning, HUANG Yue-yue, LIU Chi, MA Zeng-feng, YANG Yong . Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.[J]. Journal of Integrative Agriculture, 2012, 12(6): 962-969.

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Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.

Identification of a Resistance Gene bls1 to Bacterial Leaf Streak in Wild Rice Oryza rufipogon Griff.

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