关键词: 猪, 体细胞核移植, 氧分压, 生长因子, 培养基

Abstract: [Objective]This research focused on establishing a more efficient in vitro culture system for somatic cell nuclear transfer (SCNT) embryos. [Method] Systematically study had been carried on effect of oxygen tension（7% O2和20% O2）, culture media (NCSU-23、 PZM-3、G3) and growth factors(EGF, bFGF）on in vitro development of porcine SCNT embryos, including blastocyst formation rate and cell number per blastocyst. [Result] Compared with 20%O2, NCSU-23 in low oxygen (7%O2) greatly improved the blastocyst rate and cell number of cloned embryos（P < 0.05, 13.7±2.5% vs. 8.2±1.2%; 46.3±9.6 vs. 31.0±2.1）. cloned embryos in PZM-3 and 7 %O2 only had a greater cell number (P < 0.05, 49.4±9.8 vs. 36.6±2.1). 2-4 cell stage cloned embryos cultured in NCSU-23 supplemented with 10ng/mL EGF or bFGF had a significantly greater developmental ability to blastocyst than the control （P < 0.05, 25.8±7.6％，31.9±6.3％ vs. 15.3±8.3％）. [Conclusion] Low oxygen atmosphere improved blastocyst rate and total cell number; Regardless of atmosphere, PZM-3 supported a higher development rate and quality of porcine SCNT embryos. EGF and bFGF may be necessarily beneficial to the blastocyst formation.