UV-C对葡萄果实发育过程中黄烷醇类多酚积累及隐色花色素还原酶表达的影响

doi:10.3864/j.issn.0578-1752.2012.21.011

摘要/Abstract

摘要： 【目的】阐明葡萄果实发育过程中，植株接受UV-C照射对果实中黄烷醇类多酚积累的作用。【方法】以5年生赤霞珠葡萄为试材，定期对植株进行UV-C照射，分别采用分光光度计法、Western Blot和Real time PCR法对黄烷醇类多酚积累及其生物合成关键酶LAR表达进行分析，从底物、酶活性、蛋白质含量及基因转录水平阐明UV-C对Vv lar1、lar2基因表达的影响。【结果】葡萄果实发育过程中，UV-C照射并未改变果实中黄烷醇类多酚的积累规律，但诱导黄烷醇类多酚积累，特别是在幼果期，UV-C照射明显诱导果实中黄烷醇类多酚的积累。UV-C照射诱导葡萄果实LAR酶活性升高，LAR1、LAR2蛋白含量增加，Vv lar1、Vv lar2转录增强。【结论】UV-C能够诱导Vv lar1、Vv lar2转录，合成新LAR1、LAR2蛋白，LAR酶活性增强，导致黄烷醇类多酚在葡萄果实中积累。

关键词: 葡萄果实, UV-C, 黄烷醇类多酚, LAR

Abstract: 【Objective】 The effect of ultraviolet C on the accumulation of flavanols in grape berry during its development was studied.【Method】The 5-year old grapevine (Vitis vinifera L. cv. Cabernet Sauvignon) was subjected to the regular UV-C irradiation during berry development, and the accumulation of flavanols, the enzyme activity of leucoanthocyanidin reductase (LAR), the transcriptional level and translation level of Vv lar1 and lar2 were determined by spectrophotometer, real time PCR, and Western Blot.【Result】 The accumulation pattern of flavanols in grape berry during its development was not changed, whereas the accumulation of flavanols was promoted by UV-C irradiation, especially in the young berry. In addition, the activity of LAR, the key enzyme in the flavanols biosynthetic pathway, was induced by the UV-C irradiation. The synthesis of LAR1 and LAR2 new protein and the accumulation of Vv lar1 and lar2 mRNA induced by UV-C irradiation were also observed. 【Conclusion】 Results of the study indicated that the UV-C irradiation could induce the transcription of Vv lar1 and Vv lar2, the synthesis of LAR1 and LAR2 new protein, the increase of LAR activity, and resulted in the accumulation of flavanols in berry.

Key words: grape berry, UV-C, flavanols, LAR

温鹏飞, 邢延富, 牛铁泉, 高美英, 牛兴艳. UV-C对葡萄果实发育过程中黄烷醇类多酚积累及隐色花色素还原酶表达的影响[J]. 中国农业科学, 2012, 45(21): 4428-4436.

WEN Peng-Fei, XING Yan-Fu, NIU Tie-Quan, GAO Mei-Ying, NIU Xing-Yan. Accumulation of Flavanols, Expression of Leucoanthocyanidin Reductase Induced by UV-C Irradiation During Grape Berry Development[J]. Scientia Agricultura Sinica, 2012, 45(21): 4428-4436.

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