不同形态锰对肉仔鸡脂肪代谢关键酶活性及其基因表达的影响

doi:10.3864/j.issn.0578-1752.2011.18.016

中国农业科学 ›› 2011, Vol. 44 ›› Issue (18): 3850-3858.doi: 10.3864/j.issn.0578-1752.2011.18.016

不同形态锰对肉仔鸡脂肪代谢关键酶活性及其基因表达的影响

王美玲, 陈仲建, 吕林, 张丽阳, 罗绪刚

1. 中国农业科学院北京畜牧兽医研究所/矿物元素营养研究室/动物营养学国家重点实验室
2. 四川农业大学动物营养研究所

收稿日期:2010-08-02 修回日期:2010-11-22 出版日期:2011-09-15 发布日期:2011-09-15
通讯作者: 通信作者吕林，E-mail：lvlin1225@163.com
作者简介:王美玲，E-mail：wml0729@163.com
基金资助:
国家自然科学基金项目（30771575）、现代农业产业技术体系建设专项资金（CARS-42）、国家科技支撑计划课题（2011BAD26B04）

Effect of Different Manganese Sources on Activities and Gene Expression of Key Enzymes in Fat Metabolism of Broilers

WANG Mei-Ling, CHEN Zhong-Jian, 吕Lin , ZHANG Li-Yang, LUO Xu-Gang

1. 中国农业科学院北京畜牧兽医研究所/矿物元素营养研究室/动物营养学国家重点实验室
2. 四川农业大学动物营养研究所

Received:2010-08-02 Revised:2010-11-22 Online:2011-09-15 Published:2011-09-15

摘要/Abstract

摘要： 【目的】研究添加不同形态锰及锰水平对肉仔鸡脂肪代谢关键酶活性及其mRNA水平的影响。【方法】试验采用2×2（两种锰源×两个锰添加水平）两因子完全随机设计，另加一个不添加锰的对照组。将240只1日龄AA肉公鸡按体重随机分为5个处理组，每组设8个重复，每个重复6只鸡，分别饲喂不添加锰的玉米-豆粕型基础饲粮（对照组）和在基础饲粮中分别添加100和200 mg•kg-1锰（源于硫酸锰（MnSO4•H2O）和氨基酸锰（Mn AA））的饲粮，试验期42 d。【结果】添加锰对腹脂率无显著影响（P＞0.10），但添加锰组有降低腹脂率的趋势（P=0.103）；肝脏脂肪酸合成酶（FAS）和苹果酸脱氢酶（MDH）及腹脂激素敏感脂酶（HSL）活性不受添加锰源及锰源与锰水平互作的影响（P＞0.10），但受到添加锰水平的显著影响（P＜0.10）；添加100 mg•kg-1锰组FAS活性显著低于对照组（P＜0.10），添加100和200 mg•kg-1锰组肝脏MDH活性显著低于对照组（P＜0.01），而两个添加锰水平组间无显著差异（P＞0.10）；添加200 mg•kg-1锰组腹脂HSL活性显著高于对照组（P＜0.05）和添加100 mg•kg-1锰组（P＜0.10），添加100 mg•kg-1锰组较对照组无显著差异（P＞0.10）；添加锰源及锰源与锰水平互作对肝脏FAS和MDH及腹脂FAS和LPL mRNA水平均无显著影响（P＞0.10），但添加锰水平显著降低肝脏MDH mRNA水平（P＜0.01），而对肝脏FAS及腹脂FAS和LPL mRNA水平无显著影响（P＞0.10）；添加100 mg•kg-1和200 mg•kg-1组肝脏MDH mRNA水平都显著低于对照组（P＜0.01），但是这两个添加锰水平组间无显著差异（P＞0.10）。【结论】添加锰可以通过降低肝脏FAS和MDH活性及提高腹脂中HSL活性来降低肉仔鸡腹脂沉积，而锰可能通过降低肝脏MDH mRNA水平来降低其酶活性，有机锰和无机锰在以上作用效果方面无差异。

关键词:

锰, 脂肪酸合成酶, 苹果酸脱氢酶, 激素敏感脂酶, 脂蛋白脂酶, 肉仔鸡

Abstract: 【Objective】 The objective of the experiment is to investigate the effects of different manganese (Mn) sources and levels on activities and mRNA levels of key enzymes in fat metabolism of broilers. 【Method】 An experiment was conducted using a completely randomized design involving a 2 × 2 plus 1 arrangement of treatments (two sources of Mn×two levels of added Mn plus a control with no added Mn). A total of 240 1-d-old Arbor Acres male broilers were randomly allotted by body weight into 5 treatments (8 replicate cages of 6 chicks per cage), and fed with a corn-soybean meal basal diet and a basal diet supplemented with 100 or 200 mg•kg-1 Mn as Mn sulfate (MnSO4•H2O) or Mn amino acid (Mn AA), respectively, for a duration of 42 days. 【Result】 Supplemental manganese did not affect (P＞0.10) the percentage of abdominal fat. However, the percentage of abdominal fat from broilers fed with diet with supplemental Mn tended to decrease (P=0.103). The activities of fatty acid synthase (FAS) and malate dehydrogenase (MDH) in liver and hormone-sensitive lipase（HSL）in abdominal fat were not affected (P＞0.10) by supplemental manganese source or the interaction between Mn source and level, but were influenced (P＜0.10) by supplemental Mn level. Chicks fed diets supplemented with 100 mg•kg-1 Mn from MnSO4•H2O or Mn AA had lower (P＜0.10) FAS activities in liver than those fed with control diet. Chicks fed diets supplemented with 100 or 200 mg•kg-1 Mn had lower (P＜0.01) MDH activities in liver than those fed with control diet, and there were no differences between the two Mn level treatments. Chicks fed diets supplemented with 200 mg•kg-1 Mn had higher HSL activities in abdominal fat than those fed with the control (P＜0.05) or 100 mg•kg-1Mn diet (P＜0.10), and there were no differences between the control and 100 mg•kg-1 Mn treatments. Supplemental Mn source or the interaction between Mn source and level did not influence (P＞0.10) mRNA levels of FAS and MDH in liver or FAS and LPL in abdominal fat, but supplemental Mn level affected (P＜0.01) MDH mRNA level in liver and did not influence (P＞0.10) other enzyme mRNA levels in liver and abdominal fat. As compared with the control, supplemental 100 or 200 mg•kg-1 Mn decreased (P＜0.01) MDH mRNA level in liver, and no differences were detected between the two Mn level treatments.【Conclusion】 Supplemental Mn could reduce abdominal fat accumulation by decreasing FAS and MDH activities in liver and increasing HSL activities in abdominal fat, and Mn might decrease MDH activity in liver by decreasing MDH mRNA level in it. There were no differences in the above effect between organic Mn and inorganic Mn.

Key words:

manganese, fattyacidsynthase, malatedehydrogenase, hormone-sensitivelipase, lipoproteinlipase, broilers

王美玲, 陈仲建, 吕林, 张丽阳, 罗绪刚. 不同形态锰对肉仔鸡脂肪代谢关键酶活性及其基因表达的影响[J]. 中国农业科学, 2011, 44(18): 3850-3858.

WANG Mei-Ling, CHEN Zhong-Jian, 吕Lin , ZHANG Li-Yang, LUO Xu-Gang. Effect of Different Manganese Sources on Activities and Gene Expression of Key Enzymes in Fat Metabolism of Broilers[J]. Scientia Agricultura Sinica, 2011, 44(18): 3850-3858.

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不同形态锰对肉仔鸡脂肪代谢关键酶活性及其基因表达的影响

Effect of Different Manganese Sources on Activities and Gene Expression of Key Enzymes in Fat Metabolism of Broilers

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