2008-2010年中国华东地区PRRSV ORF5基因遗传变异分析

doi:10.3864/j.issn.0578-1752.2011.18.020

中国农业科学 ›› 2011, Vol. 44 ›› Issue (18): 3886-3894.doi: 10.3864/j.issn.0578-1752.2011.18.020

2008-2010年中国华东地区PRRSV ORF5基因遗传变异分析

王小敏, 何孔旺, 张文文, 陈蔚, 茅爱华, 俞正玉, 温立斌, 倪艳秀, 张雪寒, 吕立新, 郭容利, 周俊明, 李彬

1.江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心
2.南京农业大学动物医学院

收稿日期:2011-04-14 修回日期:2011-06-23 出版日期:2011-09-15 发布日期:2011-08-12
通讯作者: 王小敏，Tel：025-84390331；E-mail：JAAS.WXM@Gmail.com
作者简介:王小敏，Tel：025-84390331；E-mail：JAAS.WXM@Gmail.com
基金资助:
江苏省农业科技自主创新重点领域（cx(09)619）、江苏省农业科技自主创新资金（cx(10)424）

Genetic Variation of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in East China from 2008 to 2010

WANG Xiao-Min, HE Kong-Wang, ZHANG Wen-Wen, CHEN Wei, MAO Ai-Hua, YU Zheng-Yu, WEN Li-Bin, NI Yan-Xiu, ZHANG Xue-Han, 吕Li-Xin , GUO Rong-Li, ZHOU Jun-Ming, LI Bin

1.江苏省农业科学院兽医研究所/农业部动物疫病诊断与免疫重点开放实验室/国家兽用生物制品工程技术研究中心
2.南京农业大学动物医学院

Received:2011-04-14 Revised:2011-06-23 Online:2011-09-15 Published:2011-08-12
Contact: Xiaomin Wang

摘要/Abstract

摘要： 【目的】对来自2008-2010年中国华东地区7个不同省市的疑似猪繁殖与呼吸综合征（PRRS）发病猪场送检的病料进行PRRSV检测，并对其ORF5基因进行测序，确定当前PRRSV在中国华东地区的分子流行病学特征，并分析PRRSV在中国的遗传演化规律。【方法】利用RT-PCR方法对采集的样品进行PRRSV检测，并对PRRSV检测呈阳性的36份病料进行ORF5基因的序列测定和分析。【结果】260份病料中共检测到118份PRRSV阳性样品，阳性率为45.4%。ORF5序列分析和系统进化树分析结果表明，本试验所获得的36株PRRSV毒株序列均属于北美型，与GenBank中高致病性PRRSV（HP-PRRSV）参考毒株的氨基酸同源性为94.6%-100%，并可分为5个亚群，亚群III与HP-PRRSV同源性最高，几乎所有的亚群中和表位（primary neutralizing epitope，PNE）都存在变异，亚群III和IV相对于其它亚群具有更多的糖基化位点。由于36个毒株是于2008-2010年间采集自安徽、江苏、上海等7省，进化树分析结果表明，病毒的分型并没有呈现明显的地域性。【结论】2008-2010年间中国华东地区普遍存在PRRSV感染，PRRSV流行株为HP-PRRSV，其遗传演化相对稳定，但也具有不同的遗传特征。来源于不同省市的PRRSV毒株的遗传关系存在交叉，虽然亚群IV和V只出现在部分省市，但PRRSV的分布没有呈现明显的地域特征。

关键词:

猪繁殖与呼吸综合征（PRRS）, 华东地区, ORF5基因, 遗传变异

Abstract: 【Objective】 A total of 260 swine samples of dead or sick pigs obtained from Jiangsu, Anhui, Shanghai, Shandong, Zhejiang, Fujian and Jiangxi provinces of China during 2008-2010 were detected for PRRSV. And ORF5 genes of some isolates were amplified and sequenced for understanding the molecule epidemiology and the genetic evolution of porcine reproductive and respiratory syndrome virus (PRRSV) in East China.【Method】PRRSV was identified by RT-PCR from samples. Sequences of ORF5 of 36 PRRSV positive samples was amplified and analyzed with other 15 strains available on NCBI GenBank.【Result】 A total of 118 samples were positive and the positive rate was 45.4%. Sequence analysis showed that the 36 isolates of this study belong to the North American-type PRRSV strains and were closely related to the highly pathogenic PRRSV (HP-PRRSV) with 94.6-100% amino acid sequence identities. The sequence analysis combined with the phylogenetic analysis indicated that all these North American-type PRRSV strains in East China were further divided into five subgenotypes, subgenotype III showed colser identity with HP-PRRSV; almost all subgenotypes were found to be variable in the primary neutralizing epitope and subgenotypes III and IV had more glycosylation sites than others. Although these 36 isolates were collected from different provinces in East China, there were no obvious relations between the distribution of PRRSV and the region.【Conclusion】 The PRRSV infection was widespread and HP-PRRSV was the popular strain in East China during 2008-2010. However some different genetic characteristics appeared in the genomes, the genetic evolution was relatively stable. There exists a cross-cutting phenomenon on the genetic relationship of PRRSV isolates obtained from different provinces. Subgenotype IV and V only appeared in some provinces, but the distribution of PRRSV did not show apparent geographical characteristics.

Key words:

porcinereproductiveandrespiratorysyndrome(PRRS), EastChina, ORF5gene, geneticvariation

中图分类号:

S855.3

王小敏, 何孔旺, 张文文, 陈蔚, 茅爱华, 俞正玉, 温立斌, 倪艳秀, 张雪寒, 吕立新, 郭容利, 周俊明, 李彬. 2008-2010年中国华东地区PRRSV ORF5基因遗传变异分析[J]. 中国农业科学, 2011, 44(18): 3886-3894.

WANG Xiao-Min, HE Kong-Wang, ZHANG Wen-Wen, CHEN Wei, MAO Ai-Hua, YU Zheng-Yu, WEN Li-Bin, NI Yan-Xiu, ZHANG Xue-Han, 吕Li-Xin , GUO Rong-Li, ZHOU Jun-Ming, LI Bin. Genetic Variation of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in East China from 2008 to 2010[J]. Scientia Agricultura Sinica, 2011, 44(18): 3886-3894.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2011.18.020

https://www.chinaagrisci.com/CN/Y2011/V44/I18/3886

参考文献

[1]Hall W. Porcine reproductive and respiratory syndrome (PRRS) virus——a significant disease of pigs. Australian Veterinary Journal, 2005, 83 (5): 260-261.

[2]Keffaber K K. Reproductive failure of unknown etiology. American Association of Swine Practitioners (News), 1989, 1: 1-9.

[3]Wensvoort G, Terpstra C, Pol J M, ter Laak E A, Bloemraad M, de Kluyver E P, Kragten C, van Buiten L, den Besten A, Wagenaar F. Mystery swine disease in the Netherlands: the isolation of Lelystad virus. Veterinary Quarterly, 1991, 13 (3): 121-130.

[4]郭宝清, 陈章水, 刘文兴, 崔益洙. 从疑似PRRSV感染的流产胎儿分离猪生殖和呼吸综合征病毒(PRRSV)的研究. 中国畜禽传染病, 1996, 87 (2): 1-5.

Guo B Q, Chen Z S, Liu W X, Cui Y Z. Porcine reproductive and respiratory syndrome virus was isolated from abortive fetus of suspected PRRS. Chinese Journal of Animal and Poultry Infectious Disease, 1996, 87 (2): 1-5. (in Chinese)

[5]Tian K G, Yu X L, Zhao T Z, Feng Y J, Cao Z, Wang C B, Hu Yan, Chen X Z, Hu D M, Tian X S, Liu D, Zhang S, Deng X Y, Ding Y Q, Yang L, Zhang Y X, Xiao H X, Qiao M M, Wang B, Hou LL, Wang X Y, Yang X Y, Kang L P, Sun M, Jin P, Wang S J, Kitamura Y, Yan J H, Gao G F. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PloS ONE, 2007, 2 (6): 526-539.

[6]Tong G Z, Zhou Y J, Hao X F, Tian Z J, An T Q, Qiu H J. Highly pathogenic porcine reproductive and respiratory syndrome, China. Emerging Infecioust Diseases, 2007, 13 (9): 1434-1436.

[7]邹敏, 吴发兴, 王福军, 李金积, 冯阳, 刘蕾, 李明义, 陈克正, 范根成, 李晓成. 2007-2009年部分猪群PRRSV ORF5和Nsp2基因的克隆与序列分析. 西北农林科技大学学报(自然科学版), 2010, 38: 7-14.

Zou M, Wu F X, Wang F J, Li J J, Feng Y, Liu L, Li M Y, Chen K Z, Fan G C, Li X C. Cloning and sequence analysis of ORF5 and Nsp2 genes of porcine reproductive and respiratory syndrome virus from some pig farms in 2007-2009. Journal of Northwest A & F University (Nat. Sci. Ed.), 2010, 38: 7-14. (in Chinese)

[8]Rossow K D. Porcine reproductive and respiratory syndrome. Veterinary Pathology, 1998, 35: 1-20.

[9]Meulenberg J J, Petersen den Besten A, De Kluyver E, Nieuwstadt A, Wensvoort G, Moormann R J. Molecular characterization of Lelystad virus. Veterinary Microbiology, 1997, 55: 197-202.

[10]Meulenberg J J. PRRSV, the virus. Veterinary Research, 2000, 31: 11-21.

[11]Wu W H, Fang Y, Farwell R, Steffen-Bien M, Rowland R R, Christopher-Hennings J, Nelson E A. A 10 kDa structural protein of porcine reproductive and respiratory syndrome virus encoded by ORF2b. Virology, 2001, 287: 183-191.

[12]Tjeerd G K, Lisette A C, Rob J M, Rebel J M, Stockhofe-Zurwieden N. Challenges for porcine reproductive and respiratory syndrome virus (PRRSV) vaccinology. Vaccine, 2009, (27): 3704-3718.

[13]Kim W I, Yoon K J. Molecular assessment of the role of envelope associated structural proteins in cross neutralization among different PRRS viruses. Virus Genes, 2008, 37 (3): 380-391.

[14]Plagemann P G. Porcine reproductive and respiratory syndrome virus:origin hypothesis. Emerging Infectious Diseases, 2003, 9: 903-908.

[15]Meng X J, Paul P S, Halbur P G, Morozov I. Sequence comparison of open reading frames 2 to 5 of low and high virulence United States isolates of porcine reproductive and respiratory syndrome virus. The Journal of General Virology, 1995, 76 (Pt12): 3181-3188.

[16]An T Q, Zhou Y J, Liu G Q, Tian Z J, Li J, Qiu H J, Tong G Z. Genetic diversity and phylogenetic analysis of glycoprotein 5 of PRRSV isolates in mainland China from 1996 to 2006: Coexistence of two NA-subgenotypes with great diversity. Veterinary Microbiology, 2007, 123: 43-52.

[17]Mateu E, Diaz I. The challenges of PRRS immunology. The Veterinary Journal, 2008, 177: 345-351.

[18]Zhou Y J, Yu H, Tian Z J, Li G X, Hao X F, Yan L P, Peng J M, An T Q, Xu A T, Wang Y X, Wei T C, Zhang S R, Cai X H, Feng L, Li X, Zhang G H, Zhou L J, Tong G Z. Genetic diversity of the ORF5 gene of porcine reproductive and respiratory syndrome virus isolates in China from 2006 to 2008. Virus Research, 2009, 144: 136-144.

[19]Chen N H, Cao Z, Yu X L, Deng X Y, Zhao T Z, Wang L L, Liu Q, Li X X, Tian K G. Emergence of novel Europe genotype PRRSV in Mainland China. Journal of General Virology, 2011, doi: 10.1099/vir.0.027995-0.

[20]Feng Y J, Zhao, T Z, Nguyen T, Inui K, Ma Y, Nguyen T H, Nguyen V C, Liu D, Bui Q A, To L T, Wang C B, Tian K G, Gao G F. Porcine respiratory and reproductive syndrome virus variants, vietnam and China, 2007. Emerging Infectious Diseases, 2008, 14 (11): 1774-1776.

[21]Normile D. China, Vietnam grapple with ‘rapidly evolving’ pig virus. Science, 2007, 317: 1017.

[22]Zhou Y J, Hao X F, Tian Z J, Tong G Z, Yoo D, An T Q, Zhou T, Li G X, Qiu H J, Wei T C, Yuan X F. Highly virulent porcine reproductive and respiratory syndrome virus emerged in China. Transboundary Emerging Diseases, 2008, 55 (3-4): 152-164.

[23]Cha S H, Choi E J, Park J H, Yoon S R, Song J Y, Kwon J H, Song H J, Yoon K J. Molecular characterization of recent Korean porcine reproductive and respiratory syndrome (PRRS) viruses and comparison to other Asian PRRS viruses. Veterinary Microbiology, 2006, 117: 248-257.

[24]Indik S, Schmoll F, Sipos W, Klein D. Genetic variability of PRRS virus in Austria: consequences for molecular diagnostics and viral quantification. Veterinary Microbiology, 2005, 107: 171-178.

[25]Stadejek T, Oleksiewicz M B, Potapchuk D, Podgorska K. Porcine reproductive and respiratory syndrome virus strains of exceptional diversity in Eastern Europe support the definition of new genetic subtypes. Journal of General Virology, 2006, 87: 1835-1841.

[26]张建武, 庄金山, 袁世山. 中国部分地区高致病性猪繁殖与呼吸综合征病毒的分子流行病学研究. 中国农业科学, 2008, 41 (6): 1822-1831.

Zhang J W, Zhuang J S, Yuan S S. Molecular epidemiology study on high pathogenic porcine reproductive and respiratory syndrome virus in some regions of China. Scientia Agricultura Sinica, 2008, 41 (6): 1822-1831. (in Chinese)

[27]Han J, Wang Y, Faaberg K S. Complete genome analysis of RFLP184 isolates of porcine reproductive and respiratory syndrome virus. Virus Research, 2006, 122: 175-182.

[28]Wei X, Decker J M, Wang S, Hui H, Kappes JC, Wu X, Salazar-Gonzalez J F, Salazar M G, Kilby J M, Saag M S, Komarova N L, Nowak M A, Hahn B H, Kwong P D, Shaw G M. Antibody neutralization and escape by HIV-1. Nature, 2003, 422: 307-312.

[29]Plagemann P G. The primary GP5 neutralization epitope of North American isolates of porcine reproductive and respiratory syndrome virus. Veterinary Immunology and Immunopathology, 2004, 102: 263-275.

[30]Plagemann P G, Rowland R R，Faaberg K S. The primary neutralization epitope of porcine respiratory and reproductive syndrome virus strain VR-2332 is located in the middle of the GP5 ectodomain. Archies of Virology, 2002, 147 (12): 2327-2347.

[31]Ostrowski M, Galeota J A, Jar A M, Platt K B, Osorio F A, Lopez O J. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. Journal of Virology, 2002, 76: 4241-4250.

2008-2010年中国华东地区PRRSV ORF5基因遗传变异分析

Genetic Variation of the ORF5 Gene of Porcine Reproductive and Respiratory Syndrome Virus in East China from 2008 to 2010

PDF

赞

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 2

Metrics

本文评价

推荐阅读 0

[1]	. 中国部分地区2005－2007年猪繁殖与呼吸综合征病毒分离株ORF5基因和Nsp2基因遗传变异分析[J]. 中国农业科学, 2009, 42(5): 1805-1812 .
[2]	温建新,李俊,周顺,任慧英,刘文华,邹玲,徐文,牛钟相,王金宝,吴家强. 含CpG序列PRRSV ORF5基因真核质粒构建及其免疫原性的研究[J]. 中国农业科学, 2008, 41(5): 1503-1510 .