中国农业科学 ›› 2011, Vol. 44 ›› Issue (4): 657-663 .doi: 10.3864/j.issn.0578-1752.sas-2010-06863
• 作物遗传育种·种质资源·分子遗传学 • 下一篇
李晨,闫晓红,杨洁,杨清,魏文辉
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LI Chen, YAN Xiao-hong, YANG Jie, YANG Qing, WEI Wen-hui
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摘要： 植物基因工程技术中的多基因转化及转基因安全已经成为其研究的2个重要方面。植物人工染色体可以在一条不含标记基因的附加染色体上提供稳定的多基因表达,是新一代的转基因载体。由于植物人工染色体独立于宿主染色体,为植物育种提供了便利,同时,也为研究染色质特殊区域的结构与功能提供了平台。本文就植物人工染色体的产生、研究现状及其应用前景等进行了综述和讨论。
关键词: 植物人工染色体, 位点特异性重组, 微小染色体, 基因叠加, 转基因安全
Abstract:
The simultaneous expression of multiple genes and genetically modified (GM) food security research have been considered as the research focus for genetic engineering in plants presently. Unlike conventional gene transformation technologies, plant artificial chromosomes provide one solution to the stable expression of multiple transgenes. As plant artificial chromosome segregation is independent of host chromosomes, they provide a platform for accelerating plant breeding and for studying the specific chromatin domains inserted into them. The generation of artificial plant chromosomes and their applications were reviewed in this article.
Key words: plant artificial chromosome, site-specific recombination, minichromosome, gene stacking, genetically modified food security
李晨,闫晓红,杨洁,杨清,魏文辉 . 植物人工染色体：下一代基因工程的载体[J]. 中国农业科学, 2011, 44(4): 657-663 .
LI Chen,YAN Xiao-hong,YANG Jie,YANG Qing,WEI Wen-hui. Plant Artificial Chromosome: The Vector for the Next Generation of Genetic Engineering[J]. Scientia Agricultura Sinica, 2011, 44(4): 657-663 .
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