关键词: 类黄酮3′-羟化酶, 过表达, 共抑制

Abstract:

【Objective】 The objective of this study is to identify the function of F3′H gene from Eupatorium adenophorum in the secondary metabolism pathway. 【Method】 The over-expression vector of F3′H gene was constructed, and transformed into tobacco, furthermore, semi-quantitative PCR and real-time PCR were used in the study to detect the status of F3′H gene.【Result】 Although F3′H gene could be expressed in the transgenic tobacco normally, it was suppressed after flowering. The color of flowers became less bright in transgenic tobaccos than in wild-type tobaccos, at the same time, the level of F3′H gene was lower in transgenic tobacco than in wild-type tobaccos. 【Conclusion】 The result suggested that F3′H gene has close relation with the formation of anthocyanins in the secondary metabolism pathway. Expression of F3′H gene affected with endogenous F3′H gene each other after it was transferred into tobaccos, which lead to slight loss of color in flowers.

Key words: flavonoid 3′-hydrolyase, over-expression, co-suppression