不同采收期川丹皮多组分化学成分变化规律研究

中国农业科学 ›› 2006, Vol. 39 ›› Issue (05): 997-1003 .

不同采收期川丹皮多组分化学成分变化规律研究

白志川

收稿日期:2006-01-25 修回日期:1900-01-01 出版日期:2006-05-10 发布日期:2006-05-10

Research on the Changing Regulation of Ingredients in Cortex Mootan Harvested at Different Stages in Chongqing

Received:2006-01-25 Revised:1900-01-01 Online:2006-05-10 Published:2006-05-10

摘要/Abstract

摘要： 【目的】明确不同采收期川丹皮药材多组分化学成分的变化规律，确定川丹皮最佳生长期与采收期，并为丹皮药材建立一种更符合中医理论的质量控制方法。【方法】运用HPLC法在273 nm测量各样品中丹皮酚含量；在242 nm建立各样品的色谱指纹图谱，并以丹皮酚成分峰为参照计算其它成分的相对含量，图表分析展现牡丹皮多组分化学成分的动态变化规律。【结果】（1）垫江牡丹皮药材中丹皮酚的平均含量为1.25%，10月份川丹皮样品丹皮酚平均含量最高；（2）川丹皮中多组分化学成分在第5年11月份达到最高，其变化规律与丹皮酚不呈正相关。【结论】中药材多组分化学成分色谱指纹图谱技术是一种全面评价中药材质量的现代仪器分析方法，可以更广泛地应用于生产、流通领域中药材的质量控制。

关键词: 川丹皮, 多组分化学成分, 色谱指纹图谱, 质量控制, 采收期

Abstract: 【Objective】The dynamic changes of multi-ingredients group in Cortex Moutan in Chongqing were studied in order to determine the best growing stage and harvesting time, and to establish a quality control method for Cortex Moutan which is more accordant with herbalist doctor theory. 【Method】 The contents of paeonol in all samples were measured and Contex Moutan samples' chromatographic fingerprints were set up at 273 nm by HPLC. The relative quantity of other ingredients were calculated using paeonol as reference substance in order to understand the dynamic process of the main ingredients in growing circle of Paeonia suffruticosa Andr. by chart analyzing.【Result】(1) The average content of paeonol is 1.25% and the content of paeonol is the highest in samples of Cortex Moutan in Chongqing harvested in Oct. and has grown for 5 years. (2) The contents of other ingredients are the highest in Nov. in the fifth year. Their dynamic changes were positively correlated with paeonol's.【Conclusion】 TCM's multi-ingredients chromatographic fingerprints technology is a modern instrument analysis method which can be used extensively for TCM's quality control. And it can be widely used in producting and circulating field.

Key words: Chuan-Cortex Moutan, multi-chemical components, Chromatographic fingerprints, quality controling, Harvesting stage

白志川. 不同采收期川丹皮多组分化学成分变化规律研究[J]. 中国农业科学, 2006, 39(05): 997-1003 .

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