连南大叶茶树种质资源生化特性分析与适制性评价

doi:10.3864/j.issn.0578-1752.2025.22.018

中国农业科学 ›› 2025, Vol. 58 ›› Issue (22): 4797-4812.doi: 10.3864/j.issn.0578-1752.2025.22.018

• 研究简报 • 上一篇

连南大叶茶树种质资源生化特性分析与适制性评价

田先先¹^,²(), 冯韶芳¹, 王青¹, 潘晨东¹, 李波¹, 方开星¹, 吴华玲¹(), 秦丹丹¹()

¹ 广东省农业科学院茶叶研究所/广东省茶树资源创新利用重点实验室，广州 510640
² 华南农业大学园艺学院，广州 510642

收稿日期:2025-03-26 接受日期:2025-06-05 出版日期:2025-11-16 发布日期:2025-11-21
通信作者:
秦丹丹，E-mail：qdd8805@163.com。
吴华玲，E-mail：wuhualing@163.com。
联系方式: 田先先，E-mail：txianx@163.com。
基金资助:
广州市科技计划(2023B03J1036); 广州市科技计划(2025A04J5448); 广州市科技计划(2025D04J0097); 广东省重点领域研发计划(2020B020220004); 现代农业产业技术体系建设专项(CARS-19); 广东省农业农村厅乡村振兴战略专项资金种业振兴项目(2024-NBH-00-001); 广东特支计划“科技创新青年拔尖人才”项目(2021TQ06N134)

Biochemical Characteristics Analysis and Suitability Evaluation of Liannan Daye Tea Germplasm Resources

TIAN XianXian¹^,²(), FENG ShaoFang¹, WANG Qing¹, PAN ChenDong¹, LI Bo¹, FANG KaiXing¹, WU HuaLing¹(), QIN DanDan¹()

¹ Tea Research Institute, Guangdong Academy of Agricultural Sciences, Guangdong Provincial Key Laboratory of Tea Plant Resources Innovation and Utilization, Guangzhou 510640
² College of Horticulture, South China Agricultural University, Guangzhou 510642

Received:2025-03-26 Accepted:2025-06-05 Published:2025-11-16 Online:2025-11-21

摘要/Abstract

摘要：

【目的】对广东连南大叶茶树资源进行系统的生化成分分析，并结合茶叶试制与感官评审，全面评估其在绿茶和红茶加工中的适制性及品质特征，为连南大叶茶树新品种的选育、地方资源的保护与开发，以及功能性茶产品的创新提供科学依据和理论支撑。【方法】研究选取了19份具有代表性的连南大叶茶树资源进行生化成分分析，测定包括水浸出物、茶多酚、氨基酸、儿茶素类、生物碱等16种关键成分的含量，结合主成分分析和聚类分析，对不同成分进行系统性评估。此外，结合感官审评结果，评估各品种绿茶和红茶的品质表现。【结果】19份连南大叶茶树种质的生化成分存在显著差异，氨基酸、茶氨酸、茶多酚、苦茶碱和咖啡碱等成分的含量变化较大，其中氨基酸、茶氨酸等含量较高，茶多酚、儿茶素等成分含量较低，且所有资源的酚氨比均小于8.0，显示其具有良好的绿茶适制性。感官审评结果表明，连南大叶制绿茶的品质普遍较好，外形、汤色、香气和滋味表现优异，尤其是连南大叶16号、29号和38号的整体品质较为突出。制红茶时，连南大叶31号和38号的品质表现也较为优秀。聚类分析将19份广东连南茶树种质资源分为3类，且筛选出特异资源8份，包括高氨基酸资源2份、高茶氨酸资源3份、高苦茶碱资源2份、低咖啡碱资源1份。【结论】不同连南大叶茶树种质资源在生化成分和感官品质上存在显著差异，特别是氨基酸、茶氨酸、苦茶碱等含量，这些差异影响了其在绿茶和红茶生产中的适制性。高氨基酸、高茶氨酸、低咖啡碱的资源表现出较好的绿茶适制性，而高苦茶碱资源则适合功能性茶产品开发。

关键词: 连南大叶, 茶树资源, 资源鉴评, 生化成分, 特异资源

Abstract:

【Objective】 This study aimed to systematically analyze the biochemical composition of Liannan Daye tea germplasm from Guangdong Province and to evaluate its suitability and quality characteristics for green tea and black tea through sensory evaluation. The findings were intended to provide the scientific support for the breeding of new Liannan Daye varieties, the conservation and utilization of local tea resources, and the development of functional tea products. 【Method】A total of 19 representative Liannan Daye tea germplasm resources were selected for biochemical composition analysis. 16 key compounds were quantified, including water extract, tea polyphenols, free amino acids, catechins, and alkaloids. Principal component analysis (PCA) and cluster analysis were employed to systematically evaluate the variability of these components. In addition, sensory evaluation was conducted to assess the quality of green teas and black teas made from each resource. 【Result】Significant differences in biochemical composition were observed among the 19 Liannan Daye tea germplasm resources. Variations were especially notable in amino acids, theanine, tea polyphenols, theacrine, and caffeine content. Most resources exhibited high levels of amino acids and theanine but relatively low content of tea polyphenols and catechins. All resources had phenol-to-amino acid ratios below 8.0, indicating good suitability for green tea production. Sensory evaluation showed that green teas made from Liannan Daye exhibited generally high quality in appearance, liquor color, aroma, and taste. In particular, Liannan Daye 16, 29, and 38 demonstrated superior overall quality. For black tea, Liannan Daye 31 and 38 also exhibited excellent performance. Cluster analysis grouped the 19 Liannan Daye tea resources into three distinct clusters and identified eight resources with unique traits, including two high amino acid content, three high theanine content, two high theacrine content, and one low caffeine content. 【Conclusion】The Liannan Daye tea germplasm resources exhibited significant diversity in both biochemical composition and sensory quality. Differences in the levels of amino acids, theanine, and theacrine directly affected their suitability for green tea or black tea production. High amino acid, high theanine, and low caffeine resources were more suitable for green tea processing, while high theacrine resources showed potential for the development of functional tea products.

Key words: Liannan Daye, tea germplasm resources, resource evaluation, biochemical components, special resources

田先先, 冯韶芳, 王青, 潘晨东, 李波, 方开星, 吴华玲, 秦丹丹. 连南大叶茶树种质资源生化特性分析与适制性评价[J]. 中国农业科学, 2025, 58(22): 4797-4812.

TIAN XianXian, FENG ShaoFang, WANG Qing, PAN ChenDong, LI Bo, FANG KaiXing, WU HuaLing, QIN DanDan. Biochemical Characteristics Analysis and Suitability Evaluation of Liannan Daye Tea Germplasm Resources[J]. Scientia Agricultura Sinica, 2025, 58(22): 4797-4812.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2025.22.018

https://www.chinaagrisci.com/CN/Y2025/V58/I22/4797

图/表 13

图1

表1

表2

表3

连南大叶茶树资源的常规生化成分分析"

茶树资源编号 Tea tree resource number	水浸出物 Water extract (%)	茶多酚 Tea polyphenol (%)	氨基酸 Amino acid (%)	茶氨酸 Theanine (%)	可溶性糖 Soluble sugar (%)	酚氨比 Polyphenol- amino acid ratio	咖啡碱 Caffeine (%)	苦茶碱 Theacrine (%)	可可碱 Theobromine (%)
连南大叶8号 Liannan Daye 8	37.55±0.02	15.01±0.43	6.9±0.10	2.91±0.06	3.02±0.03	2.18±0.27	0.46±0	4.38±0.04	0.01±0
连南大叶11号 Liannan Daye 11	34.65±0.04	9.9±0.27	8.17±0.16	3.74±0.05	3.44±0.05	1.21±0.21	2.4±0.02	0.04±0	—
连南大叶12号 Liannan Daye 12	37.31±0.02	12.3±0.06	6.03±0.11	2.11±0.07	3±0.05	2.04±0.09	3.75±0.02	0.05±0	—
连南大叶14号 Liannan Daye 14	34.99±0.01	14.36±0.31	5.23±0.14	1.99±0.03	3.99±0.04	2.75±0.22	1.07±0.04	1.83±0.03	—
连南大叶15号 Liannan Daye 15	36.67±0.11	14.37±0.64	5.72±0.09	2.28±0.07	3.03±0.07	2.51±0.36	3.37±0.03	0.21±0	—
连南大叶16号 Liannan Daye 16	33.97±0.12	12.82±0.42	4.13±0.14	1.01±0.01	3.98±0.04	3.1±0.28	3.02±0.01	0.11±0	—
连南大叶23号 Liannan Daye 23	35.63±0.02	12.39±0.49	8.28±0.03	3.96±0.11	2.77±0.12	1.5±0.26	3.13±0.06	0.08±0	—
连南大叶27号 Liannan Daye 27	42.33±0.02	18.18±0.63	3.79±0.07	1.33±0.03	3.58±0.03	4.8±0.35	3.62±0.02	0.25±0	—
连南大叶29号 Liannan Daye 29	39.63±0.01	14.25±0.16	5.17±0.06	1.53±0.03	3.07±0.01	2.76±0.11	3.65±0.01	0.13±0	—
连南大叶31号 Liannan Daye 31	42.32±0.05	17.83±0.05	4.12±0.01	0.99±0.09	3.85±0.06	4.33±0.03	3.33±0.05	0.2±0	—
连南大叶36号 Liannan Daye 36	37.97±0.22	13.8±0.29	5.26±0.07	1.89±0.03	3.12±0.02	2.62±0.18	3.57±0.02	0.11±0	—
连南大叶38号 Liannan Daye 38	38.3±0.31	15.67±0.2	4.79±0.04	2.05±0.06	3.6±0.05	3.27±0.12	3.21±0.02	0.24±0.01	—
连南大叶40号 Liannan Daye 40	37±0.19	14.59±0.42	5.16±0.06	2.47±0.04	3.15±0.01	2.83±0.24	3.57±0.04	0.32±0.01	—
连南大叶42号 Liannan Daye 42	35.64±0.22	14.13±0.70	4.77±0.15	2.34±0.04	2.91±0.06	2.96±0.43	3.13±0.00	0.19±0	—
连南大叶45号 Liannan Daye 45	34.64±0.32	14.3±0.90	5.27±0.09	2.42±0.05	2.85±0.04	2.71±0.50	3.79±0.07	0.14±0	0.09±0.03
连南大叶49号 Liannan Daye 49	37.29±0.02	14.23±0.10	5.55±0.11	2.59±0.06	3.61±0.05	2.56±0.10	2.88±0.01	0.24±0.01	—
连南大叶50号 Liannan Daye 50	35.3±0.12	13.66±0.59	5.8±0.09	2.34±0.16	2.96±0.02	2.36±0.34	3.19±0.04	0.24±0	0.01±0
连南大叶51号 Liannan Daye 51	30.65±0.08	8.02±0.72	6.36±0.18	3.2±0.12	2.81±0.05	1.26±0.45	2.63±0.02	0.04±0	—
连南大叶52号 Liannan Daye 52	32.64±0.02	11.1±0.59	6.1±0.05	2.26±0.1	3.06±0.06	1.82±0.32	2.89±0	0.01±0	—
平均值 Average	36.55	13.73	5.61	2.29	3.25	2.61	2.98	0.46	0.01
标准差 Standard deviation	2.92	2.4	1.21	0.8	0.4	0.91	0.87	1.03	0.03
变异系数 Coefficient of variation	7.99	17.48	21.57	34.93	12.3	34.87	29.19	223.91	3.00

表3

表4

表5

连南大叶茶树资源儿茶素组分含量分析"

茶树资源编号 Tea tree resource number	GC (%)	EGC (%)	C (%)	EC (%)	EGCG (%)	GCG (%)	ECG (%)	CG (%)	儿茶素总量 Total catechins content	非酯型儿茶素 Non-ester catechins	酯型儿茶素 Ester catechins
连南大叶8号 Liannan Daye 8	0.94±0.02	2.57±0.03	0.17±0.01	0.84±0.01	6.99±0.04	0.2±0.00	2.53±0.02	0.06±0	14.3±0.02	4.52±0.07	9.78±0.06
连南大叶11号 Liannan Daye 11	0.14±0	1.3±0.02	0.03±0	0.75±0.01	4.64±0.06	0.16±0.00	2.34±0.05	0.04±0	9.4±0.02	2.22±0.03	7.18±0.11
连南大叶12号 Liannan Daye 12	0.17±0.01	1.74±0.03	0.04±0	0.6±0	6.91±0.05	0.23±0.00	1.73±0.01	0.05±0	11.47±0.01	2.55±0.04	8.92±0.06
连南大叶14号 Liannan Daye 14	0.71±0.02	1.41±0.02	0.25±0.01	0.68±0.01	6.24±0.17	0.96±0.03	2.65±0.06	0.06±0	12.96±0.04	3.05±0.06	9.91±0.26
连南大叶15号 Liannan Daye 15	0.55±0.01	1.05±0.01	0.28±0	0.49±0.01	5.42±0.07	1.27±0.01	2.65±0.04	0.08±0	11.79±0.02	2.37±0.03	9.42±0.12
连南大叶16号 Liannan Daye 16	0.7±0	1.4±0.01	0.36±0	1.16±0	3.82±0.02	0.76±0.01	2.62±0.01	0.09±0	10.91±0.01	3.62±0.01	7.29±0.04
连南大叶23号 Liannan Daye 23	0.29±0.01	2.48±0.06	0.04±0	0.83±0.02	5.88±0.13	0.32±0.02	1.3±0.06	0.06±0	11.2±0.04	3.64±0.09	7.56±0.21
连南大叶27号 Liannan Daye 27	0.96±0.01	2.25±0.02	0.25±0	0.65±0.01	8.31±0.06	1.24±0.01	2.36±0.02	0.08±0	16.1±0.02	4.11±0.04	11.99±0.09
连南大叶29号 Liannan Daye 29	0.66±0.02	1.33±0	0.11±0	0.35±0.02	6.75±0.07	1.05±0.02	1.56±0.02	0.07±0	11.88±0.02	2.45±0.04	9.43±0.11
连南大叶31号 Liannan Daye 31	1.23±0.04	2.15±0.06	0.19±0.01	0.73±0.02	8.4±0.17	0.35±0.01	1.96±0.06	0.07±0	15.08±0.05	4.3±0.13	10.78±0.24
连南大叶36号 Liannan Daye 36	0.63±0.01	1.43±0.06	0.13±0	0.41±0	6.65±0.06	0.83±0.02	1.61±0.03	0.05±0	11.74±0.02	2.6±0.07	9.14±0.11
连南大叶38号 Liannan Daye 38	0.94±0	1.74±0.03	0.24±0.01	0.57±0.02	6.05±0.14	1.19±0.05	1.8±0.05	0.06±0	12.59±0.04	3.49±0.06	9.1±0.24
连南大叶40号 Liannan Daye 40	0.76±0.02	1.1±0.03	0.19±0.01	0.31±0.01	6.86±0.09	1.34±0.02	1.61±0.02	0.08±0	12.25±0.03	2.36±0.07	9.89±0.13
连南大叶42号 Liannan Daye 42	0.92±0	1.72±0.01	0.16±0	0.45±0	6.86±0.04	1.25±0.01	1.17±0.01	0.07±0	12.6±0.01	3.25±0.01	9.35±0.06
连南大叶45号 Liannan Daye 45	0.81±0.02	1.42±0.05	0.18±0	0.39±0.01	6.27±0.18	1.3±0.04	1.49±0.04	0.06±0	11.92±0.04	2.8±0.08	9.12±0.26
连南大叶49号 Liannan Daye 49	1.1±0.01	1.33±0.01	0.24±0	0.43±0	5.84±0.11	1.4±0.03	1.68±0.02	0.05±0	12.07±0.02	3.1±0.02	8.97±0.16
连南大叶50号 Liannan Daye 50	1.34±0.03	1±0.01	0.24±0.01	0.33±0.01	5.89±0.09	0.32±0.01	1.96±0.04	0.08±0	11.16±0.03	2.91±0.06	8.25±0.14
连南大叶51号 Liannan Daye 51	0.36±0	1.15±0.01	0.05±0	0.33±0	3.25±0.07	0.39±0.01	0.82±0.02	0.05±0	6.4±0.01	1.89±0.01	4.51±0.1
连南大叶52号 Liannan Daye 52	0.26±0	2.42±0.01	0.03±0	0.66±0	5.91±0.04	0.29±0	1.4±0.01	0.06±0	11.03±0.01	3.37±0.01	7.66±0.05
平均值 Average	0.71	1.63	0.17	0.58	6.15	0.78	1.85	0.71	11.94	3.08	8.86
标准差 Standard deviation	0.35	0.51	0.1	0.22	1.28	0.47	0.54	0.35	2.04	0.73	1.59
变异系数 Coefficient of variation	49.29	31.28	58.82	37.93	20.81	60.25	29.19	16.67	17.09	23.7	17.94

表5

表6

图2

表7

表8

连南大叶茶树资源绿茶感官审评结果"

资源编号 Resource number	外形 Shape	香气 Aroma	汤色 Liquor color	滋味 Flavour	叶底 Infused tea leaves
连南大叶8号 Liannan Daye 8	黄绿有毫 Yellowish green, with pekoe	栗香甜香、高长持久 Chestnut and sweet aroma, long-lasting	黄绿明亮 Yellowish green, bright	苦味重 Strong bitterness	黄绿、柔软 Yellowish green, soft
连南大叶11号 Liannan Daye 11	黄绿带毫 Yellowish green, withlittle pekoe	甜香微花香 Sweet aroma with floral hints	黄绿明亮 Yellowish green, bright	甜醇 Sweet and mellow	黄绿、柔软 Yellowish green, soft
连南大叶12号 Liannan Daye 12	黄绿带毫 Yellowish green, with slightly pekoe	栗香 Chestnut aroma	浅黄绿明亮 Light yellowish green, bright	醇和 Mellow	黄绿、柔软 Yellowish green, soft
连南大叶14号 Liannan Daye 14	黄绿 Yellowish green	青香 Fresh grassy aroma	黄绿明亮 Yellowish green, bright	浓厚带苦味 Heavy and thick, with bitterness	黄绿、柔软 Yellowish green, soft
连南大叶15号 Liannan Daye 15	黄绿带毫 Yellowish green, with little pekoe	栗香 Chestnut aroma	黄绿明亮 Yellowish green, bright	甜醇 Sweet and mellow	绿黄、柔软 Greenish yellow, soft
连南大叶16号 Liannan Daye 16	黄绿有毫 Yellowish green with pekoe	花甜香、栗香、持久 Floral-sweet and chestnut aroma, lasting	黄绿明亮 Yellowish green, bright	甜醇鲜爽 Sweet, mellow and brisk	绿黄、柔软 Greenish yellow, soft
连南大叶23号 Liannan Daye 23	黄绿带毫 Yellowish green, with little pekoe	栗香 Chestnut aroma	黄绿明亮 Yellowish green, bright	甜醇 Sweet and mellow	黄绿、柔软 Yellowish green, soft
连南大叶27号 Liannan Daye 27	黄绿有毫 Yellowish green with pekoe	栗香 Chestnut aroma	黄绿明亮 Yellowish green, bright	浓醇带涩 Heavy and mellow with astringency	黄绿、柔软 Yellowish green, soft
连南大叶29号 Liannan Daye 29	黄绿有毫 Yellowish green with pekoe	花甜香、清长 Floral -sweet aroma, clean and lasting	绿黄明亮 Greenish yellow and bright	甜醇鲜爽 Sweet, mellow and brisk	黄绿、柔软 Yellowish green, soft
连南大叶31号 Liannan Daye 31	黄绿带毫 Yellowish green, with little pekoe	青草 Grassy aroma	黄绿明亮 Yellowish green, bright	醇和 Mellow	黄绿、柔软 Yellowish green, soft
连南大叶36号 Liannan Daye 36	黄绿 Yellowish green	栗香 Chestnut aroma	杏黄明亮 Apricot, bright	浓醇爽 Heavy, mellow and brisk	黄绿、柔软 Yellowish green, soft
连南大叶38号 Liannan Daye 38	黄绿带毫 Yellowish green, with little pekoe	花甜香细长 Delicate floral sweet aroma	黄绿明亮 Yellowish green, bright	浓醇带涩 Heavy and mellow with astringency	黄绿、柔软 Yellowish green, soft
连南大叶40号 Liannan Daye 40	黄绿有毫 Yellowish green with pekoe	微花甜香 Faint floral-sweet aroma	黄绿明亮 Yellowish green, bright	浓醇带涩 Heavy and mellow with astringency	黄、柔软 Yellow, soft
连南大叶42号 Liannan Daye 42	黄绿有毫 Yellowish green with pekoe	微花甜香 Faint floral-sweet aroma	浅黄绿明亮 Light yellowish green and bright	醇和带涩 Heavy and mellow with astringency	黄绿、柔软 Yellowish green, soft
连南大叶45号 Liannan Daye 45	黄绿带毫 Yellowish green, with little pekoe	栗香 Chestnut aroma	浅黄绿明亮 Light yellowish green, bright	醇和 Mellow	黄绿、柔软 Yellowish green, soft
连南大叶49号 Liannan Daye 49	黄绿有毫 Yellowish green with pekoe	栗香 Chestnut aroma	浅黄绿明亮 Light yellowish green, bright	甜醇 Sweet and mellow	绿黄、较柔软 Greenish yellow, soft
连南大叶50号 Liannan Daye 50	黄绿带毫 Yellowish green,little pekoe	栗香 Chestnut aroma	浅黄绿明亮 Light yellowish green, bright	醇和 Mellow	绿黄、较柔软 Greenish yellow, soft
连南大叶51号 Liannan Daye 51	黄绿带毫 Yellowish green, with little pekoe	栗香 Chestnut aroma	浅黄绿明亮 Light yellowish green, bright	醇和 Mellow	绿黄、较柔软 Greenish yellow, soft
连南大叶52号 Liannan Daye 52	黄绿带毫 Yellowish green, with little pekoe	栗香 Chestnut aroma	浅黄绿明亮 Light yellowish green, bright	甜醇 Sweet and mellow	绿黄、柔软 Greenish yellow, soft

表8

图3

表9

连南大叶茶树资源红茶感官审评结果"

资源编号 Resource number	外形 Appearance	香气 Aroma	汤色 Liquor color	滋味 Taste	叶底 Brewed leaves
连南大叶8号 Liannan Daye 8	乌润略带金毫 Black bloom, with little golden pekoe	薯甜香尚浓持久 Sweet potato aroma, moderately strong and lasting	橙红明亮 Orange red, bright	滋味平和、极苦 Mellow, with bitterness	褐红、较亮 Auburn red, relatively bright
连南大叶12号 Liannan Daye 12	乌润紧细、略带金毫 Black bloom, tight and slender, with little golden pekoey	甜香 Sweet aroma approach	橙黄明亮 Orange yellow, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright
连南大叶14号 Liannan Daye 14	乌润略带金毫 Black bloom, with little golden pekoe	甜香 Sweet aroma	深橙红明亮 Deep orange red, bright	极苦 Extremely bitterness	褐红、较亮 Auburn red, relatively bright
连南大叶15号 Liannan Daye 15	乌润略带金毫 Black bloom, with little golden pekoe	木香较低沉 Mellow woody aroma	橙红明亮 Orange red, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright
连南大叶16号 Liannan Daye 16	乌润略带金毫 Black bloom, with little golden pekoe	甜香、浓郁持久 Sweet aroma, strong and lasting	橙黄明亮 Orange yellow, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright
连南大叶23号 Liannan Daye 23	乌润紧细、略带金毫 Black bloom, tight and slender, with little golden pekoe	甜香 Sweet aroma	橙红明亮 Orange red, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright
连南大叶27号 Liannan Daye 27	乌润紧细、有毫 Black bloom, tight and slender, have golden pekoe	甜香 Sweet aroma	深橙红明亮 Deep orange red, bright	醇和 Mellow	红亮 Red and bright
连南大叶31号 Liannan Daye 31	乌润略带金毫 Black bloom, with little golden pekoe	焦糖香，高长持久 Caramel, strong and lasting	橙红明亮 Orange red, bright	带红糖甜香、醇和 Mellow, with sugar sweetness,	褐红、较亮 Auburn red, relatively bright
连南大叶36号 Liannan Daye 36	乌润油亮 Black bloom	木香 Woody aroma	橙红明亮 Orange red, bright	较醇和 Relatively mellow	褐红、较亮 Auburn red, relatively bright
连南大叶38号 Liannan Daye 38	乌褐略带金毫 Black bloom, with little golden pekoe	花甜香较细腻 Delicate floral-sweet aroma	橙红明亮 Orange red, bright	带甜香、醇和 Mellow, with little sweetness,	褐红、较亮 Auburn red, relatively bright
连南大叶40号 Liannan Daye 40	乌润略带金毫 Black bloom, with little golden pekoe	木香、略带花甜香 Woody aroma with little floral-sweet aroma	深橙红明亮 Deep orange red, bright	醇和 Mellow	红亮 Red and bright
连南大叶42号 Liannan Daye 42	乌润紧细 Black bloom, tight and slender	木香 Woody aroma	橙红明亮 Orange red, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright
连南大叶49号 Liannan Daye 49	乌润紧细、有金毫 Black bloom, tight and slender, have golden pekoe	木香 Woody aroma	橙红明亮 Orange red, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright
连南大叶51号 Liannan Daye 51	乌褐有金毫 Black auburn, have golden pekoe	木香 Woody aroma	橙红明亮 Orange red, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright
连南大叶52号 Liannan Daye 52	乌润带金毫 Black bloom, have golden pekoe	木香 Woody aroma	橙黄明亮 Orange yellow, bright	醇和 Mellow	褐红、较亮 Auburn red, relatively bright

表9

图4

参考文献 31

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洗脱时间 Elution time (min)	0	0.35	13.40	13.50	15.70	15.80	18.00
流动相A Mobile phase A (%)	93.5	93.5	43.0	0	0	93.5	93.5
流动相B Mobile phase B (%)	6.5	6.5	57.0	100	100	6.5	6.5

资源类型 Resource type	资源名称 Resource name
高氨基酸 High amino acid (≥7.0%)	连南大叶11号（8.17%）、连南大叶23号（8.28%） Liannan Daye 11 (8.17%), Liannan Daye 23 (8.28%)
高茶氨酸 High theanine (≥3.0%)	连南大叶11号（3.74%）、连南大叶23号（3.96%）、连南大叶51号（3.20%） Liannan Daye 11 (3.74%), Liannan Daye 23 (3.96%), Liannan Daye 51 (3.20%)
高苦茶碱 High theacrine (>1.5%)	连南大叶8号（4.38%）、连南大叶14号（1.83%） Liannan Daye 8 (4.38%), Liannan Daye 14 (1.83%)
低咖啡碱 Low caffeine (<1.0%)	连南大叶8号（0.46%） Liannan Daye 8 (0.46%)

生化成分 Biochemical component	PC1	PC2	PC3	PC4
水浸出物 Water extract	0.77	0.04	0.47	-0.10
茶多酚 Tea polyphenol	0.95	0.05	0.27	0.09
氨基酸 Amino acid	-0.81	0.40	0.15	0.11
茶氨酸 Theanine	-0.79	0.21	0.14	0.32
咖啡碱 Caffeine	0.12	-0.81	0.26	-0.42
苦茶碱 Theacrine	0.15	0.78	0.01	0.52
可溶性糖 Soluble sugar	0.58	0.24	-0.47	-0.25
GC	0.74	-0.09	0.00	0.43
EGC	0.13	0.63	0.54	-0.33
C	0.75	-0.08	-0.57	0.20
EC	0.13	0.71	-0.32	-0.55
EGCG	0.66	0.10	0.70	0.02
GCG	0.50	-0.55	-0.11	0.33
ECG	0.49	0.52	-0.49	-0.01
CG	0.59	-0.15	-0.36	-0.18
儿茶素总量 Total catechins content	0.87	0.29	0.36	0.03
特征值 Eigenvalue	6.31	3.14	2.35	1.41
贡献率 Contribution rate (%)	39.40	19.65	14.67	8.81
累积贡献率 Cumulative contribution rate (%)	39.40	59.05	73.72	82.53

类群 Group	资源 Resource	水浸出物 Water extract (%)	茶多酚 Tea polyphenol (%)	氨基酸 Amino acid (%)	茶氨酸 Theanine (%)	咖啡碱 Caffeine (%)	苦茶碱 Theacrine (%)	儿茶素总量 Total catechins content (%)	酚氨比 Polyphenol - amino acid ratio
1	连南大叶14、15、18、27、29、31、36、38、40、42、45、49、50号 Liannan Daye 14, 15, 18, 27, 29, 31, 36, 38, 40, 42, 45, 49, 50	33.97—42.33	12.82—18.18	3.79—5.80	0.99—2.59	1.07—3.79	0.11—1.83	9.36—13.82	2.36—4.80
平均值 Average		37.39	14.78	4.98	1.94	3.18	0.32	10.58	3.04
2	连南大叶11、12、23、51、52号 Liannan Daye 11, 12, 23, 51, 52	30.65—37.31	8.02—12.39	6.03—6.99	2.11—3.96	2.4—3.75	0.01—0.08	5.60—11.02	1.21—2.04
平均值 Average		34.18	10.74	6.99	3.05	2.96	0.05	9.33	1.57
3	连南大叶8号 Liannan Daye 8	37.55	15.01	6.9	2.91	0.46	4.38	13.10	2.18

连南大叶茶树种质资源生化特性分析与适制性评价

Biochemical Characteristics Analysis and Suitability Evaluation of Liannan Daye Tea Germplasm Resources

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