中国农业科学 ›› 2005, Vol. 38 ›› Issue (02): 425-427 .

• 研究简报 • 上一篇    下一篇

李矮缩病毒RT-PCR方法建立及检测应用

杨俊玲,侯义龙,李雄   

  1. 内蒙古农业大学农学院
  • 收稿日期:2004-02-03 修回日期:1900-01-01 出版日期:2005-02-10 发布日期:2005-02-10
  • 通讯作者: 杨俊玲

Establishment and Application of Detection of Prune Dwarf Virus (PDV) by RT-PCR

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  1. 内蒙古农业大学农学院
  • Received:2004-02-03 Revised:1900-01-01 Online:2005-02-10 Published:2005-02-10

摘要: 以感病和健康指示植物GF305的总RNA为模板,进行cDNA的合成和PCR 扩增,结果从感病材料中扩增出与预期的172 bp大小一致的目的片段,而健康的材料无此扩增产物。对此PCR 扩增产物克隆测序,进一步佐证了RT-PCR检测结果。经过多次试验验证该检测体系,都可得到很好的重演性,从而建立了李矮缩病毒快速、灵敏、准确的RT-PCR检测技术,并进行了实际应用。

关键词: 李矮缩病毒, 逆转录-聚合酶链式反应, 克隆与测序

Abstract: A pair of primers was synthesized based on the nucleotide sequence of the coat protein gene of prune dwarf virus (PDV).The expected size 172bp was amplified by RT-PCR from the infected samples, while no amplified products from the healthy samples.The amplified products were cloned and sequenced. The result showed that the detection system was stable.Thus a rapid, sensitive and accurate method for PDV identification and detection by RT-PCR has been built up and were used to detect whether some fruit trees in orchards were infected by PDV or not.

Key words: Prune dwarf virus, RT-PCR, Cloning and sequencing