甜椒L 3应对辣椒轻斑驳病毒及中椒系列新品种的选育

doi:10.3864/j.issn.0578-1752.2020.18.018

摘要/Abstract

摘要：

辣（甜）椒是我国栽培面积最大的蔬菜作物,年播种面积约2.2×10⁶ hm²,其中甜椒约5×10⁵hm²。生产上传统病害如疫病、病毒病等依然严峻,近年来辣椒轻斑驳病毒（PMMoV,烟草花叶病毒属）等新型流行病害爆发,严重制约甜椒生产;同时,消费者对品种的品质、多样性提出更高要求。笔者课题组先后从国内外引进甜（辣）椒种质资源1 400余份,通过鉴定、评价,筛选出具有抗病毒病、白粉病和果大、皮薄、光泽度好、品质优等优良性状的种质资源120余份。构建了与抗TMV（L³、L⁴）、抗番茄斑点萎蔫病毒TSWV（Tsw）等重要性状紧密连锁的分子标记,辅助育种准确率达90%以上。通过常规育种技术和分子标记相结合,创制出含有抗PMMoV（P_0,1,2）L³,且兼具抗TMV、ToMV、PMMoV（P_0,1,2）、CMV和疫病,果大、果实均一度高、光泽度好等综合性状优良、配合力高的甜椒骨干亲本‘0516’,以其为骨干亲本,培育出4个新一代优质、多抗、适应不同生态区的新品种‘中椒105号’‘中椒106号’‘中椒107号’‘中椒108号’。上述系列新品种含有L ³,抗TMV、PMMoV（P_0,1,2),兼抗CMV和疫病;果实形状大小、色泽、整齐度等商品品质,Vc等营养品质显著提高。

关键词: 甜椒, 辣椒轻斑驳病毒病, 新品种选育

Abstract:

Hot (sweet) pepper is the largest area vegetable crop in China, with an annual planting area of 2.2×10⁶ hm ², including 5×10⁵ hm² of sweet pepper. Beside traditional diseases, in recent years, pepper milt mottle virus (PMMoV) as a new popular disease broke out, which seriously restricted the production of sweet pepper. At the same time, consumers put forward higher requirements for the quality and diversity of varieties. Our research group has introduced more than 1 400 accessions of hot (sweet) pepper germplasm resources from all over the world. Through identification and evaluation, more than 120 accessions with good characteristics such as antiviral disease, powdery mildew, large fruit, thin skin, good glossiness and high quality have been screened out. The molecular markers closely linked with TMV (L³, L⁴), TSWV (Tsw) and other important characters were constructed. The accuracy of assisted breeding was over 90%. Through the combination of conventional breeding techniques and molecular markers, the core backbone parental sweet pepper inbred line ‘0516’ was created with good combining ability, resistant to TMV, ToMV, PMMoV (P_0,1,2), CMV and Phytophthora capsici, large fruit, uniform and good glossiness, which contained L³ gene resistant to PMMoV (P_0,1,2). Four new varieties Zhongjiao 105, 106, 107 and 108 with high quality, multi resistance and adaptability to different ecological areas were developed. These new varieties contained L³ gene, which was resistant to TMV, PMMoV (P_0,1,2), CMV and disease; the shape, size, color, uniformity and other commodity quality, vitamin C and other nutritional quality of fruits were significantly improved. It was important to improve the disease resistance and quality of sweet pepper.

Key words: sweet pepper, capsicum, pepper milt mottle virus (PMMoV), breeding of new varieties

张宝玺,王立浩,张正海,曹亚从,于海龙. 甜椒L ³应对辣椒轻斑驳病毒及中椒系列新品种的选育[J]. 中国农业科学, 2020, 53(18): 3846-3855.

ZHANG BaoXi,WANG LiHao,ZHANG ZhengHai,CAO YaCong,YU Hailong. Development of New Varieties of ZHONGJIAO Pepper Resistant to Pepper Mild Mottle Virus Using L ³Gene[J]. Scientia Agricultura Sinica, 2020, 53(18): 3846-3855.

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链接本文: https://www.chinaagrisci.com/CN/10.3864/j.issn.0578-1752.2020.18.018

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引进地区 Introduction of the region	年份 Year			合计 Total
引进地区 Introduction of the region	2001—2005	2006—2010	2010—2015	合计 Total
中国各省、市、区Chinese provinces, cities and districts	230	322	336	888
欧美 Europe and America	101	156	181	438
日本Japan	12	2	1	15
南亚South Asia	1	10	8	19
其他Other	22	31	7	60
合计Total	367	521	533	1420

材料特性 Material property	来源 Source	份数 Number	代表种质 Typical germplasm
抗TMV TMV-resistant	中国、法国、日本、美国等 China, France, Japan, America, etc	21	Alliance;斯玛特;考曼其 Alliance;Smart; Kaomanchi
抗CMV CMV-resistant	泰国、法国等 Thailand, France, etc	9	OrientE3 (MaxzxMax3) 51-39; OrientE3 (MaxzxMax3) 51-39b; OrientE3(Max1xNana)
抗疫病 Phytophthora-resistant	泰国、法国等 Thailand, France, etc	10	RP30; RP38; RP40; RP4
抗炭疽 Anthracnose-resistant	亚蔬中心 Asian Vegetable Research and Development Center	5	PBC932; PBC81
抗疮痂 Scab-resistant	中国、泰国、法国等 China, Thailand, France, etc	7	PS09979325 XCR10;PBC446
抗PVY PVY-resistant	中国、法国、美国等 China, France, America, etc	21	Alliance;科曼;斯特朗 Alliance;Keman; Sitelang
抗青枯 Bacterial wilt-resistant	中国等 China, etc	13	马来西亚椒;肉厚牛角椒;PBC204 Malaysian; Thick horn pepper; PBC204
抗CVMV CVMV-resistant	中国等 China, etc	12	GTCV1; GTCV2; GTCV3
抗蓟马 Thrip-resistant	荷兰等 Holland, etc	10	Bisbas; Ac 1979
抗线虫 Nematode-resistant	法国等 France, etc	5	DH330; DH149; CM334
耐低温、弱光 Tolerance to low temperature and weak light	荷兰等 Holland, etc	7	37-74; 37-82等
耐寒 cold-resistant	美国 America	3	Florist type; Poinsettia
其他 Other	中国、俄罗斯、日本等 China, Russia, Japan, etc	9

抗病种类 Disease-resistant species	基因名称 Gene name	显/隐性 Dominant/ Recessive	位座 Chromosomal location	连锁标记 Linkage marker	参考文献 Reference
烟草花叶病毒Tobacco mosaic virus	L^3,4	显性Dominant	P11	SCAR	[27-28]
番茄斑点萎蔫病毒Tomato spot wilt virus	Tsw	显性Dominant	P10	CAPS	[29-30]
黄瓜花叶病毒Cucumber mosaic virus	QTL (2)	共显性Codominant	P12	KASP-UN54228	[31]
马铃薯Y病毒Potato virus Y	Pvr4	显性Dominant	P10	CAPS	[32-33]
疮痂病 Bacterial spot	Bs2	显性Dominant	-	SCAR-A2	[27,34]
疮痂病 Bacterial spot	Bs3	显性Dominant	P2	SCAR	[27,35]
疫病 Phytophthora capsici	QTL (18)	共显性Codominant	P5	KASP-5-160	专利技术,未发表 Patented technology and unpublished
炭疽病Anthracnose	QTL (P5.1)	共显性Codominant	P5	KASPUN27353	[18]
白粉病Powdery mildew	QTL (5)	共显性Codominant	P6	SSRH6-96	[36]
根结线虫 Root-knot nematode	N	显性Dominant	P9	SCAR	[25]
根结线虫 Root-knot nematode	Me1	显性Dominant	P9	16830CAPS	[37]

组合 Combination	亩产 Acre yield (kg/667 m²)	组合 Combination	亩产 Acre yield (kg/667 m²)
A×B	4257.68	B×F	4118.73
A×C	3741.87	C×D	3774.11
A×D	3551.78	C×E	4404.98
A×E	4518.93	C×F	4035.35
A×F	4353.29	D×E	4669.00
B×C	4302.15	D×F	4452.23
B×D	4624.53	E×F	3251.63
B×E	4741.81

材料 Material	GCA
AF5	-101.828
0516	222.442
1421	-134.846
RQN	27.792
KLS	130.732
0601M	-144.292

甜椒L ³应对辣椒轻斑驳病毒及中椒系列新品种的选育

Development of New Varieties of ZHONGJIAO Pepper Resistant to Pepper Mild Mottle Virus Using L ³Gene

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